Acute effects of prostaglandin F(2alpha) on systemic oxytocin and progesterone concentrations during the mid- or late-luteal phase in mares.
Abstract: The acute effects of prostaglandin F(2alpha) (PGF) on circulating oxytocin and progesterone concentrations were characterized in mares during the mid- or late-luteal phase. Pony mares were randomly assigned to the following experimental groups based on treatment with PGF (2.5mg) or saline on Day 8 or Day 13 (Day 0=ovulation): PGF-8, PGF-13, saline-8, or saline-13 (n=7/group). Mares were fitted with indwelling, jugular vein catheters and two blood samples (-5 and 0 min) were collected prior to treatment. Treatments were administered into the jugular vein (0 min) and blood collection continued thereafter at 1 min intervals until 5 min and then at 5 min intervals until 60 min. Based on the combined data of -5 and 0 min samples, mares on Day 8 had greater (P<0.05) oxytocin concentrations than mares on Day 13. On Day 8, PGF treatment resulted in a biphasic pattern of oxytocin release. Oxytocin concentrations increased (P<0.05) 1 min after PGF treatment, decreased (P<0.05) from 1 to 10 min, and increased (P<0.05) from 10 to 30 min. Oxytocin concentrations were greater (P<0.05) from 1 to 3 min in PGF-treated than saline-treated mares and at most sample times from 15 to 60 min. On Day 13, oxytocin concentrations were greater (P<0.05) in PGF-treated than in saline-treated mares for most sample times. Mares treated with PGF on Day 8 had greater (P<0.05) oxytocin concentrations at 25, 30, and 40 min than mares on Day 13. Progesterone concentrations on Day 8 also increased by 1 min after PGF, decreased toward basal concentrations by 2-3 min, and then increased to a maximum 10 min after treatment. Subsequently, circulating progesterone decreased (P<0.05) below pretreatment concentrations by 40-50 min after PGF. In conclusion, treatment with PGF resulted in an immediate and biphasic increase in progesterone concentrations prior to the expected decrease. Treatment of mares with PGF on Day 8 resulted in an overall greater increase in systemic oxytocin concentrations compared to treatment on Day 13, and the increase on Day 8 was biphasic.
Publication Date: 2006-02-24 PubMed ID: 16500050DOI: 10.1016/j.anireprosci.2006.01.009Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article investigates the immediate effects of a hormone called prostaglandin F(2alpha) (PGF) on the levels of two other hormones, oxytocin and progesterone, in mares (female horses) at two different points in time (Day 8 and Day 13) after ovulation.
Methods
- Female mares used for this study were categorized into four groups based on the treatment they received (PGF or saline) and the day the treatment was administered (Day 8 or Day 13).
- The mares were kept under observation and blood samples were collected at specific intervals both before and after the treatment was administered.
Findings
- The intensity of oxytocin in the mares was comparatively higher on Day 8 than on Day 13.
- The effect of PGF treatment on oxytocin was biphasic (having two phases) in nature, such that it led to an increase in oxytocin levels one minute post-treatment, this was followed by a drop and then another increase.
- The effect of PGF treatment on progesterone followed a similar pattern.
Conclusion
- The results indicate that PGF treatment led to a biphasic increase in progesterone levels before the anticipated decrease.
- Furthermore, PGF treatment on Day 8 led to a greater increase in oxytocin levels compared to treatment on Day 13.
This study contributes to understanding the impact of the hormonal agent, prostaglandin F(2alpha), on female reproductive physiology, particularly the luteal phase of the ovulation cycle. Its findings can have future applications in veterinary and reproductive biology.
Cite This Article
APA
Utt MD, Acosta TJ, Wiltbank MC, Ginther OJ.
(2006).
Acute effects of prostaglandin F(2alpha) on systemic oxytocin and progesterone concentrations during the mid- or late-luteal phase in mares.
Anim Reprod Sci, 97(1-2), 63-73.
https://doi.org/10.1016/j.anireprosci.2006.01.009 Publication
Researcher Affiliations
- Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison 53706, USA.
MeSH Terms
- Animals
- Dinoprost / pharmacology
- Female
- Horses / blood
- Kinetics
- Luteal Phase / blood
- Luteal Phase / drug effects
- Oxytocics / pharmacology
- Oxytocin / blood
- Oxytocin / drug effects
- Oxytocin / metabolism
- Progesterone / blood
- Random Allocation
Citations
This article has been cited 2 times.- Kaps M, Okada CTC, Gautier CM, Aurich J, Aurich C. Deslorelin Slow-Release Implants Delay Ovulation and Increase Plasma AMH Concentration and Small Antral Follicles in Haflinger Mares.. Animals (Basel) 2021 May 28;11(6).
- Shah KB, Tripathy S, Suganthi H, Rudraiah M. Profiling of luteal transcriptome during prostaglandin F2-alpha treatment in buffalo cows: analysis of signaling pathways associated with luteolysis.. PLoS One 2014;9(8):e104127.
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