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Home / Equine Research Bank / Vet Immunol Immunopathol / Acute phase protein haptoglobin as inflammatory marker in se...
Veterinary immunology and immunopathology2016; 182; 74-78; doi: 10.1016/j.vetimm.2016.10.005

Acute phase protein haptoglobin as inflammatory marker in serum and synovial fluid in an equine model of arthritis.

Abstract: Acute phase proteins are useful inflammatory markers in horses. Haptoglobin (Hp) serum level is increased in horses undergoing different inflammatory processes, including arthritis. However, Hp concentration has not been assessed in inflammatory synovial fluid (SF). The aim of the present study was to investigate the Hp response in serum and SF in horses undergoing experimentally induced arthritis. For this purpose, serum and SF samples were collected from 12 animals before amphotericin B-induced arthritis was created (T0, healthy) and 15days after the lesion induction (T1, joint inflammation) and Hp was determined by single radial immunodiffusion. The Hp increase between T0 and T1 was significant in both serum and SF, and serum Hp concentration at T0 was significantly higher than in SF, but significant differences were not found at T1, indicating a higher Hp increase in SF. A significant positive correlation for Hp concentration between serum and SF samples was found. These results highlight the potential usefulness of Hp as inflammatory marker in horses, showing for the first time the increase of Hp in SF from joint inflammation in the horse.
Copyright © 2016 Elsevier B.V. All rights reserved.
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Publication Date: 2016-10-17 PubMed ID: 27863554DOI: 10.1016/j.vetimm.2016.10.005Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study on haptoglobin (Hp), an acute phase protein, as an inflammatory marker in horses. The study explored the presence of Hp in serum and synovial fluid in horses with experimental induced arthritis.

Objective of the Study

  • The main objective of this study was to investigate the Hp response in both serum and synovial fluid in horses that underwent experimentally induced arthritis. This was aimed at ascertaining the potential usefulness of Hp as a novel inflammatory marker for arthritis in horses.

Study Design and Methodology

  • The researchers collected serum and synovial fluid samples from 12 horses before the induction of arthritis (referred to as T0 in this study, indicating the healthy baseline) and 15 days post lesion induction (referred to as T1, indicative of joint inflammation).
  • Amphotericin B, a type of drug known to induce inflammatory changes, was used to create arthritis in the horses.
  • The haptoglobin levels were then determined in the samples using a technique known as single radial immunodiffusion, which allows for the measurement of specific proteins.

Key Findings

  • Results demonstrated a significant increase in Hp levels both in serum and synovial fluid from T0 to T1, indicating that inflammation due to arthritis leads to elevated Hp concentration.
  • While the Hp concentration in serum was significantly higher than in synovial fluid at T0, no significant difference was found at T1, suggesting a greater Hp increase in synovial fluid.
  • A significant positive correlation was also identified for Hp concentration between serum and synovial fluid samples, implying that an increase of Hp in serum is indicative of an increase of Hp in synovial fluid as well.

Conclusion and Significance

  • This is the first study of its kind to show increased Hp concentration in synovial fluid from joint inflammation in horses. This highlights the potential of Hp as an effective inflammatory marker in horses suffering from arthritis.
  • The study’s findings could contribute to improved detection and monitoring of inflammatory processes like arthritis in horses, potentially leading to more effective treatment protocols.

Cite This Article

APA
Barrachina L, Remacha AR, Soler L, García N, Romero A, Vázquez FJ, Vitoria A, Álava MÁ, Lamprave F, Rodellar C. (2016). Acute phase protein haptoglobin as inflammatory marker in serum and synovial fluid in an equine model of arthritis. Vet Immunol Immunopathol, 182, 74-78. https://doi.org/10.1016/j.vetimm.2016.10.005

Publication

Veterinary immunology and immunopathology
ISSN: 1873-2534
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 182
Pages: 74-78
PII: S0165-2427(16)30202-1

Researcher Affiliations

Barrachina, Laura
  • Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, C/Miguel Servet, 177, 50013 Zaragoza, Spain.
Remacha, Ana Rosa
  • Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain.
Soler, Lourdes
  • Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, C/Pedro Cerbuna, 12, 50009, Zaragoza, Spain.
García, Natalia
  • Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, C/Pedro Cerbuna, 12, 50009, Zaragoza, Spain.
Romero, Antonio
  • Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, C/Miguel Servet, 177, 50013 Zaragoza, Spain.
Vázquez, Francisco José
  • Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, C/Miguel Servet, 177, 50013 Zaragoza, Spain.
Vitoria, Arantza
  • Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, C/Miguel Servet, 177, 50013 Zaragoza, Spain.
Álava, María Ángeles
  • Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, C/Pedro Cerbuna, 12, 50009, Zaragoza, Spain.
Lamprave, Fermín
  • Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, C/Pedro Cerbuna, 12, 50009, Zaragoza, Spain.
Rodellar, Clementina
  • Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain. Electronic address: rodellar@unizar.es.

MeSH Terms

  • Animals
  • Arthritis / blood
  • Arthritis / metabolism
  • Arthritis / veterinary
  • Arthritis, Experimental / blood
  • Arthritis, Experimental / metabolism
  • Biomarkers / blood
  • Biomarkers / metabolism
  • Haptoglobins / metabolism
  • Horse Diseases / blood
  • Horse Diseases / metabolism
  • Horses
  • Inflammation Mediators / blood
  • Inflammation Mediators / metabolism
  • Male
  • Synovial Fluid / metabolism
  • Time Factors

Citations

This article has been cited 6 times.
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    doi: 10.3390/ani11102952pubmed: 34679973google scholar: lookup
  2. Chakrabarti S, Ai M, Henson FMD, Smith ESJ. Peripheral mechanisms of arthritic pain: A proposal to leverage large animals for in vitro studies. Neurobiol Pain 2020 Aug-Dec;8:100051.
    doi: 10.1016/j.ynpai.2020.100051pubmed: 32817908google scholar: lookup
  3. Tothova C, Mihajlovicova X, Novotny J, Nagy O, Giretova M, Kresakova L, Tomco M, Zert Z, Vilhanova Z, Varga M, Medvecky L, Petrovova E. The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect. Materials (Basel) 2019 Jan 3;12(1).
    doi: 10.3390/ma12010142pubmed: 30609876google scholar: lookup
  4. Barrachina L, Remacha AR, Romero A, Vitoria A, Albareda J, Prades M, Roca M, Zaragoza P, Vázquez FJ, Rodellar C. Assessment of effectiveness and safety of repeat administration of proinflammatory primed allogeneic mesenchymal stem cells in an equine model of chemically induced osteoarthritis. BMC Vet Res 2018 Aug 17;14(1):241.
    doi: 10.1186/s12917-018-1556-3pubmed: 30119668google scholar: lookup
  5. Ma TW, Li Y, Wang GY, Li XR, Jiang RL, Song XP, Zhang ZH, Bai H, Li X, Gao L. Changes in Synovial Fluid Biomarkers after Experimental Equine Osteoarthritis. J Vet Res 2017 Dec;61(4):503-508.
    doi: 10.1515/jvetres-2017-0056pubmed: 29978116google scholar: lookup
  6. Andreassen SM, Vinther AML, Nielsen SS, Andersen PH, Tnibar A, Kristensen AT, Jacobsen S. Changes in concentrations of haemostatic and inflammatory biomarkers in synovial fluid after intra-articular injection of lipopolysaccharide in horses. BMC Vet Res 2017 Jun 19;13(1):182.
    doi: 10.1186/s12917-017-1089-1pubmed: 28629364google scholar: lookup
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