Adhesive properties of platelets from different animal species.
Abstract: The use of large animals (e.g., pig and sheep) in human medicine, and the need to develop new therapeutic strategies for domestic animal diseases related to platelet disorders, require better characterization of the physiology of animal platelets. In this study, the ability of platelets from buffaloes, horses, pigs and sheep to adhere to immobilized autologous fibrinogen was compared with that of human platelets. Blood samples were collected from the jugular vein of six healthy subjects of each species and platelet-rich plasma (PRP) was obtained by centrifugation. Platelets, isolated by further centrifugation of PRP, were washed by gel-filtration on Sepharose-2B, counted and added to the wells of 96-well plates pre-coated with autologous fibrinogen. After different times of incubation, non-adherent platelets were removed, and the number of adherent platelets was assessed by measuring endogenous acid phosphatase activity. Horse platelets showed the strongest ability to adhere to autologous immobilized fibrinogen, being 1.7-, 3.1- and 2.3-fold more active than human, buffalo and porcine platelets, respectively. Sheep platelets were unable to adhere to autologous immobilized fibrinogen. Platelet activation by adenosine 5-diphosphate (ADP) increased both human and animal platelet adhesive response. ADP-stimulated sheep platelets were able to adhere to autologous immobilized fibrinogen, albeit to a lesser extent than platelets from the other animal species. The observed interspecies variability in adhesive properties of platelets may reflect structural differences, or differences in the availability of the fibrinogen receptor (glycoprotein IIb/IIIa) on the platelet surface.
Publication Date: 2003-03-14 PubMed ID: 12634089DOI: 10.1053/jcpa.2002.0615Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigates the ability of platelets from different animal species to adhere to their own fibrinogen, compared to human platelets. The findings revealed varying levels of adhesive strength, with horse platelets showing the highest and sheep platelets showing no adherence in an unstimulated state.
Understanding the Methodology
- Platelets from buffaloes, horses, pigs, sheep, and humans were compared in this study.
- Blood samples were drawn from the jugular vein of six healthy individuals of each species.
- The blood was centrifuged to obtain platelet-rich plasma (PRP).
- These platelets were further isolated by centrifugation, washed, counted, and added to plates that were pre-treated with fibrinogen from the same species.
- The platelets were then incubated for varying periods, following which non-adherent platelets were removed.
- The number of platelets that adhered to the fibrinogen was measured by assessing endogenous acid phosphatase activity (enzyme present inside the platelets).
Observations and Findings
- Horse platelets exhibited the highest ability to adhere to their own fibrinogen. They were 1.7 times more active than human platelets and 3.1 and 2.3 times more active than buffalo and pig platelets, respectively.
- Sheep platelets did not adhere to autologous (originating from the same organism) immobilized fibrinogen in the unstimulated state.
- However, when stimulated by adenosine 5-diphosphate (ADP), which is a platelet activation factor, even sheep platelets adhered to fibrinogen, albeit weaker than the other species.
- The platelet activation with ADP increased the adhesive response in all species, including humans.
Implications
- The interspecies variability in adhesive properties may be due to structural differences or differences in the availability of fibrinogen receptors (glycoprotein IIb/IIIa) on the surface of the platelets.
- This research provides valuable information for better characterizing the physiology of animal platelets, an important factor in developing new therapeutic strategies related to platelet disorders in both human and veterinary medicine.
Cite This Article
APA
Pelagalli A, Belisario MA, Tafuri S, Lombardi P, d'Angelo D, Avallone L, Staiano N.
(2003).
Adhesive properties of platelets from different animal species.
J Comp Pathol, 128(2-3), 127-131.
https://doi.org/10.1053/jcpa.2002.0615 Publication
Researcher Affiliations
- Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, Università di Napoli Federico II, Via F. Delpino 1, 80137, Napoli, Italy.
MeSH Terms
- Acid Phosphatase / analysis
- Acid Phosphatase / metabolism
- Adenosine Diphosphate / pharmacology
- Anestrus
- Animals
- Animals, Domestic / blood
- Blood Platelets / drug effects
- Blood Platelets / enzymology
- Blood Platelets / physiology
- Buffaloes
- Female
- Horses
- Platelet Adhesiveness / drug effects
- Platelet Adhesiveness / physiology
- Sheep
- Species Specificity
- Swine
Citations
This article has been cited 14 times.- Christer T, Hüner A, Robering JW, Mrowietz C, Hiebl B. Differences in human and commercial hybrid pig platelet activation induced by borosilicate glass beads in a modified chandler loop-system. Clin Hemorheol Microcirc 2024;88(s1):S35-S44.
- Jamiolkowski MA, Golding MD, Malinauskas RA, Lu Q. In Vitro Thrombogenicity Testing of Biomaterials in a Dynamic Flow Loop: Effects of Length and Quantity of Test Samples. J Med Device 2023 Sep 1;17(3):031003.
- Jamiolkowski MA, Patel M, Golding MD, Malinauskas RA, Lu Q. Comparison of animal and human blood for in vitro dynamic thrombogenicity testing of biomaterials. Artif Organs 2022 Dec;46(12):2400-2411.
- Schreiber K, Decouture B, Lafragette A, Chollet S, Bruneau M, Nicollet M, Wittmann C, Gadrat F, Mansour A, Forest-Villegas P, Gauthier O, Touzot-Jourde G. A novel autotransfusion device saving erythrocytes and platelets used in a 72 h survival swine model of surgically induced controlled blood loss. PLoS One 2022;17(3):e0260855.
- Ebert MLA, Schmidt VF, Pfaff L, von Thaden A, Kimm MA, Wildgruber M. Animal Models of Neointimal Hyperplasia and Restenosis: Species-Specific Differences and Implications for Translational Research. JACC Basic Transl Sci 2021 Nov;6(11):900-917.
- Jamiolkowski MA, Hartung MC, Malinauskas RA, Lu Q. An In Vitro Blood Flow Loop System for Evaluating the Thrombogenicity of Medical Devices and Biomaterials. ASAIO J 2020 Feb;66(2):183-189.
- Johnson CA Jr, Woolley JR, Snyder TA, Shankarraman V, Haney EI, Wagner WR. Assessment of Thrombelastography and Platelet Life Span in Ovines. Artif Organs 2018 Dec;42(12):E427-E434.
- Thiel A, Mogel H, Bruggisser J, Baumann A, Wyder M, Stoffel MH, Summerfield A, Posthaus H. Effect of Clostridium perfringens β-Toxin on Platelets. Toxins (Basel) 2017 Oct 24;9(10).
- Gruba SM, Koseoglu S, Meyer AF, Meyer BM, Maurer-Jones MA, Haynes CL. Platelet membrane variations and their effects on δ-granule secretion kinetics and aggregation spreading among different species. Biochim Biophys Acta 2015 Jul;1848(7):1609-18.
- Weigand A, Boos AM, Ringwald J, Mieth M, Kneser U, Arkudas A, Bleiziffer O, Klumpp D, Horch RE, Beier JP. New aspects on efficient anticoagulation and antiplatelet strategies in sheep. BMC Vet Res 2013 Oct 3;9:192.
- Dohmen PM. Tissue engineered aortic valve. HSR Proc Intensive Care Cardiovasc Anesth 2012;4(2):89-93.
- Honge JL, Funder JA, Pedersen TB, Kronborg MB, Hasenkam JM. Degenerative processes in bioprosthetic mitral valves in juvenile pigs. J Cardiothorac Surg 2011 May 15;6:72.
- Ye SH, Johnson CA Jr, Woolley JR, Snyder TA, Gamble LJ, Wagner WR. Covalent surface modification of a titanium alloy with a phosphorylcholine-containing copolymer for reduced thrombogenicity in cardiovascular devices. J Biomed Mater Res A 2009 Oct;91(1):18-28.
- Johnson CA Jr, Snyder TA, Woolley JR, Wagner WR. Flow cytometric assays for quantifying activated ovine platelets. Artif Organs 2008 Feb;32(2):136-45.
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