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Home / Equine Research Bank / Sci Rep / Adiposity in mares induces insulin dysregulation and mitocho...
Scientific reports2024; 14(1); 13992; doi: 10.1038/s41598-024-64628-x

Adiposity in mares induces insulin dysregulation and mitochondrial dysfunction which can be mitigated by nutritional intervention.

Abstract: Obesity is a complex disease associated with augmented risk of metabolic disorder development and cellular dysfunction in various species. The goal of the present study was to investigate the impacts of obesity on the metabolic health of old mares as well as test the ability of diet supplementation with either a complex blend of nutrients designed to improve equine metabolism and gastrointestinal health or L-carnitine alone to mitigate negative effects of obesity. Mares (n = 19, 17.9 ± 3.7 years) were placed into one of three group: normal-weight (NW, n = 6), obese (OB, n = 7) or obese fed a complex diet supplement for 12 weeks (OBD, n = 6). After 12 weeks and completion of sample collections, OB mares received L-carnitine alone for an additional 6 weeks. Obesity in mares was significantly associated with insulin dysregulation, reduced muscle mitochondrial function, and decreased skeletal muscle oxidative capacity with greater ROS production when compared to NW. Obese mares fed the complex diet supplement had better insulin sensivity, greater cell lipid metabolism, and higher muscle oxidative capacity with reduced ROS production than OB. L-carnitine supplementation alone did not significantly alter insulin signaling, but improved lipid metabolism and muscle oxidative capacity with reduced ROS. In conclusion, obesity is associated with insulin dysregulation and altered skeletal muscle metabolism in older mares. However, dietary interventions are an effective strategy to improve metabolic status and skeletal muscle mitochondrial function in older mares.
© 2024. The Author(s).
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Publication Date: 2024-06-18 PubMed ID: 38886475PubMed Central: PMC11183153DOI: 10.1038/s41598-024-64628-xGoogle Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the effects of obesity on elderly mares’ metabolic health and how diet supplementation with certain nutrients or L-carnitine alone can lessen these negative effects. The research discovered that obesity significantly associates with disturbed insulin regulation and reduced muscle mitochondrial function, which can be mitigated with dietary interventions.

Research Methodology

  • The research study used 19 mature mares, aged 17.9 ± 3.7 years, which were divided into three groups: normal-weight (NW, n = 6), obese (OB, n = 7), and obese fed a complex diet supplement for 12 weeks (OBD, n = 6).
  • After 12 weeks, samples were collected, after which the OB group was given L-carnitine alone for an additional six weeks.

Findings

  • Obesity in mares showed a significant correlation with insulin dysregulation, decreased muscle mitochondrial function, and reduced skeletal muscle oxidative capacity along with increased ROS production when compared to the normal-weight group.
  • Obese mares that were fed the complex diet supplement had better insulin sensitivity, increased cellular lipid metabolism, and enhanced muscle oxidative capacity with reduced ROS production than the obese group.
  • L-carnitine supplementation alone did not meaningfully change insulin signaling but enhanced lipid metabolism and muscle oxidative capacity, and decreased ROS.

Conclusion

  • The research concluded that obesity has a notable relationship with insulin dysregulation and changing skeletal muscle metabolism in older mares.
  • However, the application of dietary interventions featuring either a complex nutrient blend improving equine metabolism and gastrointestinal health or L-carnitine alone showed promise in preventing these negative impacts of obesity. These dietary strategies effectively improved metabolic status and muscle mitochondrial function in the older mares.

Cite This Article

APA
Fresa K, Catandi GD, Whitcomb L, Gonzalez-Castro RA, Chicco AJ, Carnevale EM. (2024). Adiposity in mares induces insulin dysregulation and mitochondrial dysfunction which can be mitigated by nutritional intervention. Sci Rep, 14(1), 13992. https://doi.org/10.1038/s41598-024-64628-x

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 14
Issue: 1
Pages: 13992
PII: 13992

Researcher Affiliations

Fresa, Kyle
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA.
Catandi, Giovana D
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA.
Whitcomb, Luke
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA.
Gonzalez-Castro, Raul A
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA.
Chicco, Adam J
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA.
Carnevale, Elaine M
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA. elaine.carnevale@colostate.edu.

MeSH Terms

  • Animals
  • Horses
  • Female
  • Insulin / metabolism
  • Insulin / blood
  • Carnitine / metabolism
  • Carnitine / pharmacology
  • Obesity / metabolism
  • Obesity / diet therapy
  • Dietary Supplements
  • Adiposity / drug effects
  • Mitochondria / metabolism
  • Mitochondria / drug effects
  • Muscle, Skeletal / metabolism
  • Muscle, Skeletal / drug effects
  • Lipid Metabolism / drug effects
  • Horse Diseases / metabolism
  • Horse Diseases / diet therapy
  • Horse Diseases / etiology
  • Insulin Resistance
  • Reactive Oxygen Species / metabolism

Grant Funding

  • Grant No. COLV 2021-09/Project Accession No. 1026913 / USDA National Institute of Food and Agriculture Animal Health and Disease

Conflict of Interest Statement

The authors declare no competing interests.

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