Aerosolized Micropolyspora faeni antigen as a cause of pulmonary dysfunction in ponies with recurrent airway obstruction (heaves).
Abstract: Ponies with recurrent airway obstruction (principal ponies) and their controls were given aerosolized Micropolyspora faeni antigen via endotracheal tube during a period when the principal ponies were in disease remission. In both groups of ponies, we performed bronchoalveolar lavage (BAL) and measured pulmonary function at base line, and 5 hours after aerosol administration of 30 ml of 0.9% NaCl solution or 30 ml of 1% w/v particulate M faeni antigen in 0.9% NaCl solution. In both groups of ponies, aerosolized M faeni antigen increased WBC count, neutrophil numbers, and albumin concentration in BAL fluid, but macrophage numbers decreased. In the principal ponies, BAL mast cell numbers were decreased 5 hours after administration of M faeni antigen. The M faeni antigen had no effect on the mechanical properties of the lungs or on gas exchange in the control ponies, but did increase respiratory frequency minute ventilation and pulmonary resistance, and decreased arterial oxygen tension in the principal ponies. Changes in pulmonary function were apparent only in the principal ponies, which suggests that neutrophils, per se, do not cause pulmonary dysfunction and that M faeni may be one of the etiologic agents involved in chronic obstructive pulmonary disease.
Publication Date: 1988-06-01 PubMed ID: 3400931
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
- Antigen
- Bronchoalveolar Lavage
- Chronic Obstructive Pulmonary Disease
- Clinical Study
- Diagnosis
- Disease Diagnosis
- Disease Etiology
- Equine Health
- Horses
- Immune Response
- Inflammation
- Lung Health
- Mast Cells
- Neutrophils
- Pathophysiology
- Pony
- Pulmonary Health
- Recurrent Airway Obstruction
- Respiratory Disease
- Veterinary Medicine
- White Blood Cells
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The article investigates how aerosolized Micropolyspora faeni, a type of fungus, can cause lung dysfunction in ponies suffering from recurrent airway obstruction. The study indicates that M. faeni might play a role in chronic obstructive pulmonary disease and that the presence of neutrophils alone does not result in lung dysfunction.
Methodology
- The researchers carried out the study using two groups of ponies: those with recurrent airway obstruction (principal ponies) and a control group. The study was conducted during a period when the principal ponies were in disease remission.
- Both sets of ponies were given aerosolized M. faeni via an endotracheal tube.
- They conducted bronchoalveolar lavage (BAL), a procedure for examining the lungs, and measured the ponies’ lung function before and 5 hours after being given two types of aerosols: a 0.9% NaCl solution or a mixture of M. faeni in a 0.9% NaCl solution.
Results
- Results showed an increase in white blood cell count, neutrophil numbers, and albumin concentration in the BAL fluid for both group of ponies after being exposed to M. faeni. However, the number of macrophages decreased.
- In the ponies with recurrent airway obstruction, the number of mast cells in the BAL fluid decreased 5 hours after administration of the M. faeni antigen.
- While the M. faeni antigen had no effect on lung function or gas exchange in the control group, it led to an increase in respiratory frequency, minute ventilation, and pulmonary resistance, and a decrease in arterial oxygen tension in the affected ponies.
Conclusions
- The research suggests that M. faeni could be one of the causes of chronic obstructive pulmonary disease, indicated by the changes only observed in the principal ponies.
- The study also indicates that the mere presence of neutrophils, a type of white blood cell, does not lead to lung dysfunction. This suggests that other factors, like M. faeni, are crucial to developing lung issues.
Cite This Article
APA
Derksen FJ, Robinson NE, Scott JS, Stick JA.
(1988).
Aerosolized Micropolyspora faeni antigen as a cause of pulmonary dysfunction in ponies with recurrent airway obstruction (heaves).
Am J Vet Res, 49(6), 933-938.
Publication
Researcher Affiliations
- Pulmonary Laboratory, College of Veterinary Medicine, Michigan State University, East Lansing 48824.
MeSH Terms
- Aerosols
- Airway Obstruction / etiology
- Airway Obstruction / veterinary
- Albumins / analysis
- Animals
- Antigens, Bacterial / immunology
- Bronchoalveolar Lavage Fluid / analysis
- Bronchoalveolar Lavage Fluid / cytology
- Cell Count / veterinary
- Horse Diseases / etiology
- Horses
- Leukocyte Count / veterinary
- Lung / physiopathology
- Macrophages
- Mast Cells
- Micromonosporaceae / immunology
- Neutrophils
- Oxygen / blood
- Recurrence
- Respiration
Grant Funding
- HL 27619 / NHLBI NIH HHS
- HL 37051 / NHLBI NIH HHS
Citations
This article has been cited 8 times.- Lo Feudo CM, Stucchi L, Alberti E, Conturba B, Zucca E, Ferrucci F. Intradermal Testing Results in Horses Affected by Mild-Moderate and Severe Equine Asthma. Animals (Basel) 2021 Jul 13;11(7).
- Dauvillier J, Ter Woort F, van Erck-Westergren E. Fungi in respiratory samples of horses with inflammatory airway disease. J Vet Intern Med 2019 Mar;33(2):968-975.
- Klier J, Geis S, Steuer J, Geh K, Reese S, Fuchs S, Mueller RS, Winter G, Gehlen H. A comparison of nanoparticullate CpG immunotherapy with and without allergens in spontaneously equine asthma-affected horses, an animal model. Immun Inflamm Dis 2018 Mar;6(1):81-96.
- Moran G, Buechner-Maxwell VA, Folch H, Henriquez C, Galecio JS, Perez B, Carrasco C, Barria M. Increased apoptosis of CD4 and CD8 T lymphocytes in the airways of horses with recurrent airway obstruction. Vet Res Commun 2011 Oct;35(7):447-56.
- Morán G, Folch H, Araya O, Burgos R, Barria M. Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay. Vet Res Commun 2010 Dec;34(8):719-26.
- Morán G, Burgos R, Araya O, Folch H. In vitro bioassay to detect reaginic antibodies from the serum of horses affected with recurrent airway obstruction. Vet Res Commun 2010 Jan;34(1):91-9.
- Naylor JM, Clark EG, Clayton HM. Chronic obstructive pulmonary disease: Usefulness of clinical signs, bronchoalveolar lavage, and lung biopsy as diagnostic and prognostic aids. Can Vet J 1992 Sep;33(9):591-8.
- Doucet MY, Vrins AA, Ford-Hutchinson AW. Histamine inhalation challenge in normal horses and in horses with small airway disease. Can J Vet Res 1991 Jul;55(3):285-93.
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