Age-dependent expression of osteochondrosis-related genes in equine leukocytes.
Abstract: Osteochondrosis (OC) is a developmental disease in horses which has a significant impact on the horse's welfare and performance. The early disturbance in the process of endochondral ossification progresses to inflammatory and repair processes in older horses. Previously, differentially expressed genes in leukocytes of OC-affected horses have been identified. The aim of the present study is to detect age-related changes in these differentially expressed genes. Methods: The expression of OC-related genes was analysed by real-time PCR and subsequent statistical analysis (ΔΔCT) in the leukocytes of 135 Belgian Warmblood horses divided into three different age groups: 30 months (n=38). Results: Relative expression of genes of horses less than 12 months of age showed significant induction of the genes MGAT4A, PRKCG, MHCI, ApoB, ApoB3G, B4GALT6 and a significantly lower expression of the genes OAS3. Horses of 18-24 months of age, showed a significantly higher expression of the genes TBC1D9, MGAT4A, IFIH1, MHCIIa and MMP1. Horses of more than 30 months of age showed a significantly higher expression of the genes MGAT4A, HP, SECTM1 compared with their age-matched control groups. Conclusions: The study demonstrates that OC-related genes are differentially expressed in horses of different ages compared with their age-matched controls. Some of the genes may be implicated in cell signalling and differentiation as well as carbohydrate and lipid metabolism and inflammation. However, the causal relationship between the differentially expressed genes and the development and progression of the OC lesions needs to be determined.
Publication Date: 2015-03-24 PubMed ID: 26392886PubMed Central: PMC4567144DOI: 10.1136/vetreco-2014-000058Google Scholar: Lookup
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Summary
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The research article focuses on the investigation of age-related changes in the expression of osteochondrosis-related genes in horses. The study suggests that these genes vary across different ages and may impact cell signalling, differentiation, and metabolism.
Background and Aim
- The study was driven by the need to further understand osteochondrosis (OC), a developmental disease in horses that disrupts endochondral ossification while significantly impacting their welfare and performance. Prior research had identified differentially expressed genes in the leukocytes of OC-affected horses.
- The primary aim of the ongoing investigation was to detect any possible age-related changes in the expression of these genes.
Methods
- The researchers used real-time PCR to analyze the expression of OC-related genes and made use of ΔΔCT for subsequent statistical analysis.
- The sample for the study comprised 135 Belgian Warmblood horses divided into three different age groups: less than 12 months (47 horses), 18-24 months (50 horses), and more than 30 months (38 horses).
Results
- The findings from the study suggest notable variations in the expression of genes across different age groups. For horses less than 12 months of age, there was significant induction of several genes (MGAT4A, PRKCG, MHCI, ApoB, ApoB3G, B4GALT6) and a notably lower expression of the OAS3 gene.
- In the case of horses aged 18-24 months, a significantly higher expression of TBC1D9, MGAT4A, IFIH1, MHCIIa, and MMP1 genes was observed. Similarly, horses older than 30 months exhibited a significantly higher expression of MGAT4A, HP, and SECTM1 genes, compared with their age-matched control groups.
Conclusions
- One of the critical insights from the study was that OC-related genes are differentially expressed in horses of different ages when compared with their age-matched peers.
- Some of these genes could potentially influence cell signalling and differentiation processes, as well as carbohydrate and lipid metabolism and inflammation.
- However, a conclusive causal relationship between these differentially expressed genes and the development and progression of OC lesions still needs to be established.
Cite This Article
APA
Mendoza L, Piquemal D, Lejeune JP, Vander Heyden L, Noguier F, Bruno R, Sandersen C, Serteyn D.
(2015).
Age-dependent expression of osteochondrosis-related genes in equine leukocytes.
Vet Rec Open, 2(1), e000058.
https://doi.org/10.1136/vetreco-2014-000058 Publication
Researcher Affiliations
- University of Liège , Liège , Belgium ; Equine Research and Development Center , Mont-le-Soie , Belgium.
- Computational Biology Institute (IBC) , France ; University of Montpellier2 , Montpellier , France ; Acobiom , Montpellier , France.
- Equine Research and Development Center , Mont-le-Soie , Belgium.
- Equine Research and Development Center , Mont-le-Soie , Belgium.
- Acobiom , Montpellier , France.
- Acobiom , Montpellier , France.
- University of Liège , Liège , Belgium.
- University of Liège , Liège , Belgium ; Equine Research and Development Center , Mont-le-Soie , Belgium.
References
This article includes 30 references
- Bertone AL, Bramlage LR, McIlwraith CW, Malemud CJ. Comparison of proteoglycan and collagen in articular cartilage of horses with naturally developing osteochondrosis and healing osteochondral fragments of experimentally induced fractures.. Am J Vet Res 2005 Nov;66(11):1881-90.
- Bertrand G, Coste J, Segarra C, Schved JF, Commes T, Marti J. Use of serial analysis of gene expression (SAGE) technology reveals new granulocytic markers.. J Immunol Methods 2004 Sep;292(1-2):43-58.
- Denoix JM, Jacquet S, Lepeule J, Crevier-Denoix N, Valette JP, Robert C. Radiographic findings of juvenile osteochondral conditions detected in 392 foals using a field radiographic protocol.. Vet J 2013 Jul;197(1):44-51.
- Desjardin C, Chat S, Gilles M, Legendre R, Riviere J, Mata X, Balliau T, Esquerré D, Cribiu EP, Betch JM, Schibler L. Involvement of mitochondrial dysfunction and ER-stress in the physiopathology of equine osteochondritis dissecans (OCD).. Exp Mol Pathol 2014 Jun;96(3):328-38.
- El-Osta A, Brasacchio D, Yao D, Pocai A, Jones PL, Roeder RG, Cooper ME, Brownlee M. Transient high glucose causes persistent epigenetic changes and altered gene expression during subsequent normoglycemia.. J Exp Med 2008 Sep 29;205(10):2409-17.
- Jacquet S, Robert C, Valette JP, Denoix JM. Evolution of radiological findings detected in the limbs of 321 young horses between the ages of 6 and 18 months.. Vet J 2013 Jul;197(1):58-64.
- Jeffcott LB. Osteochondrosis in the horse--searching for the key to pathogenesis.. Equine Vet J 1991 Sep;23(5):331-8.
- Johswich A, Longuet C, Pawling J, Abdel Rahman A, Ryczko M, Drucker DJ, Dennis JW. N-glycan remodeling on glucagon receptor is an effector of nutrient sensing by the hexosamine biosynthesis pathway.. J Biol Chem 2014 Jun 6;289(23):15927-41.
- Kamm JL, Frisbie DD, McIlwraith CW, Orr KE. Gene biomarkers in peripheral white blood cells of horses with experimentally induced osteoarthritis.. Am J Vet Res 2013 Jan;74(1):115-21.
- Laverty S, Girard C. Pathogenesis of epiphyseal osteochondrosis.. Vet J 2013 Jul;197(1):3-12.
- Lecocq M, Girard CA, Fogarty U, Beauchamp G, Richard H, Laverty S. Cartilage matrix changes in the developing epiphysis: early events on the pathway to equine osteochondrosis?. Equine Vet J 2008 Jul;40(5):442-54.
- Liew CC, Ma J, Tang HC, Zheng R, Dempsey AA. The peripheral blood transcriptome dynamically reflects system wide biology: a potential diagnostic tool.. J Lab Clin Med 2006 Mar;147(3):126-32.
- López-Orduña E, Cruz M, García-Mena J. The transcription of MGAT4A glycosyl transferase is increased in white cells of peripheral blood of type 2 diabetes patients.. BMC Genet 2007 Oct 22;8:73.
- McIlwraith CW. Inferences from referred clinical cases of osteochondrosis dissecans. Equine Veterinary Journal Supplement 16, 27–30.
- Mirams M, Tatarczuch L, Ahmed YA, Pagel CN, Jeffcott LB, Davies HM, Mackie EJ. Altered gene expression in early osteochondrosis lesions.. J Orthop Res 2009 Apr;27(4):452-7.
- Olstad K, Hendrickson EH, Carlson CS, Ekman S, Dolvik NI. Transection of vessels in epiphyseal cartilage canals leads to osteochondrosis and osteochondrosis dissecans in the femoro-patellar joint of foals; a potential model of juvenile osteochondritis dissecans.. Osteoarthritis Cartilage 2013 May;21(5):730-8.
- Rejnö S, Strömberg B. Osteochondrosis in the horse. II. Pathology.. Acta Radiol Suppl 1978;358:153-78.
- Savage CJ, McCarthy RN, Jeffcott LB. Effects of dietary energy and protein on induction of dyschondroplasia in foals. Equine Veterinary Journal 25, 74–79.
- Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method.. Nat Protoc 2008;3(6):1101-8.
- Semevolos SA, Brower-Toland BD, Bent SJ, Nixon AJ. Parathyroid hormone-related peptide and indian hedgehog expression patterns in naturally acquired equine osteochondrosis.. J Orthop Res 2002 Nov;20(6):1290-7.
- Semevolos SA, Nixon AJ, Brower-Toland BD. Changes in molecular expression of aggrecan and collagen types I, II, and X, insulin-like growth factor-I, and transforming growth factor-beta1 in articular cartilage obtained from horses with naturally acquired osteochondrosis.. Am J Vet Res 2001 Jul;62(7):1088-94.
- Semevolos SA, Strassheim ML, Haupt JL, Nixon AJ. Expression patterns of hedgehog signaling peptides in naturally acquired equine osteochondrosis.. J Orthop Res 2005 Sep;23(5):1152-9.
- Serteyn D, Piquemal D, Vanderheyden L, Lejeune JP, Verwilghen D, Sandersen C. Gene expression profiling from leukocytes of horses affected by osteochondrosis.. J Orthop Res 2010 Jul;28(7):965-70.
- Tusher VG, Tibshirani R, Chu G. Significance analysis of microarrays applied to the ionizing radiation response.. Proc Natl Acad Sci U S A 2001 Apr 24;98(9):5116-21.
- Vander Heyden L, Lejeune JP, Caudron I, Detilleux J, Sandersen C, Chavatte P, Paris J, Deliège B, Serteyn D. Association of breeding conditions with prevalence of osteochondrosis in foals.. Vet Rec 2013 Jan 19;172(3):68.
- Verwilghen DR, Janssens S, Busoni V, Pille F, Johnston C, Serteyn D. Do developmental orthopaedic disorders influence future jumping performances in Warmblood stallions?. Equine Vet J 2013 Sep;45(5):578-81.
- Yoon JC, Ng A, Kim BH, Bianco A, Xavier RJ, Elledge SJ. Wnt signaling regulates mitochondrial physiology and insulin sensitivity.. Genes Dev 2010 Jul 15;24(14):1507-18.
- Ytrehus B, Carlson CS, Ekman S. Etiology and pathogenesis of osteochondrosis.. Vet Pathol 2007 Jul;44(4):429-48.
- Yuan JS, Wang D, Stewart CN Jr. Statistical methods for efficiency adjusted real-time PCR quantification.. Biotechnol J 2008 Jan;3(1):112-23.
- Zabek T, Golonka P, Fornal A, Semik E. IHH gene polymorphism among three horse breeds and its application for association test in horses with osteochondrosis.. Hereditas 2013 Jun;150(2-3):38-43.
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