Allergen-specific ELISA for horse IgE.
Abstract: An enzyme-linked immuno sorbent assay (ELISA) for measuring horse IgE specific to ovalbumin, bencylpenicilloic acid and odinitrocarboxyphenol is described. We used a sandwich type of ELISA by which horse serum was incubated in antigen-coated tubes containing one additional polystyrene ball, followed by rabbit anti horse IgE serum. The tubes were then incubated with biotinylated goat anti rabbit globulin followed by avidin coupled to phosphatase. Endpoint titrations were compared. The ELISA is highly reproducible due to the pretreatment of the polystyrene with glutaraldehyde. The increased antigen surface area led to a higher sensitivity of the test. The assay can be easily modified for the measurement of other Ig classes and of IgE of other antigen specificities.
Publication Date: 1983-07-01 PubMed ID: 6351418DOI: 10.1016/0165-2427(83)90064-8Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article presents the successful development of a highly reliable and sensitive enzyme-linked immuno sorbent assay (ELISA) for the detection of specific horse IgE antibodies against certain allergens, with possible modifications for measuring other Ig classes and IgE of different antigens.
Overview
- This research paper is centered on the development and description of an enzymatic assay, specifically an Enzyme-Linked Immuno Sorbent Assay (ELISA), designed to measure horse Immunoglobulin E (IgE) that are specific for certain substances or antigens.
- The allergens in question for this particular study are ovalbumin (found in egg white), bencylpenicilloic acid, and dinitrocarboxyphenol.
Procedure
- The researchers employed a sandwich-type ELISA for their experiment, which is a common type of ELISA known for its high specificity.
- In the procedure, horse serum was mixed in tubes coated with the antigen, along with an additional polystyrene ball. This setup was left to incubate.
- This was then followed by a second round of incubation involving rabbit anti horse IgE serum, biotinylated goat anti rabbit globulin, and avidin connected to phosphatase.
Findings
- The gathered results or endpoint titrations of the assay were compared, presenting great reproducibility in the collaborative effort of the ELISA, much of which can be attributed to the pretreatment of the polystyrene with glutaraldehyde.
- This improved consistency, coupled with the increased antigen surface area, resulted in a significantly heightened sensitivity of the test, which is a highly desirable trait for any diagnostic assay as it enhances the level of accuracy of the test.
Additional Notes
- Beyond the promising results of the experiment, the researchers also emphasized the flexibility of their newly designed assay, noting that it can be conveniently modified for measuring other classes of Immunoglobulins (Igs), as well as for evaluating IgE that are specific for other antigens.
- This suggests potential utility in a variety of immunological studies or diagnostic applications involving horses or other species.
Cite This Article
APA
Suter M, Fey H.
(1983).
Allergen-specific ELISA for horse IgE.
Vet Immunol Immunopathol, 4(5-6), 555-564.
https://doi.org/10.1016/0165-2427(83)90064-8 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Enzyme-Linked Immunosorbent Assay
- Horses / immunology
- Immunoenzyme Techniques
- Immunoglobulin E / immunology
Citations
This article has been cited 1 times.- Suter M, Kobisch M, Nicolet J. Stimulation of immunoglobulin-containing cells and isotype-specific antibody response in experimental Mycoplasma hyopneumoniae infection in specific-pathogen-free pigs.. Infect Immun 1985 Sep;49(3):615-20.
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