Allergen-specific IgE levels against crude mould and storage mite extracts and recombinant mould allergens in sera from horses affected with chronic bronchitis.
Abstract: Immunoglobulin E antibody (IgE) levels against four recombinant (r) mould allergens (r-Aspergillus fumigatus [rAsp f] 7, 8 and 9; r-Alternaria alternata 1 [rAlta1]) and crude mould (Aspergillus fumigatus, Alternaria alternata, Penicillium notatum) and storage mite extracts were determined by ELISA in sera from 24 pulmonary sound control horses and 26 horses suffering from chronic bronchitis/bronchiolitis (CB), also called chronic obstructive pulmonary disease (COPD). Serum IgG and IgA titres were also determined against Aspergillus fumigatus extract and rAsp f 8.IgE against the crude extracts could be measured in all sera, but there was no significant difference between CB-affected and control horses. In contrast, only 8-30% of the horses, depending on the r-allergen tested, had detectable IgE levels in serum against the r-allergens. Horses with CB had significantly more often detectable IgE levels than controls against rAlt a 1 (10/26 and 3/24, respectively, p=0. 054), rAsp f 7 (13/26 and 2/24, respectively, p<0.01) and rAsp f 8 (11/26 and 1/24, respectively, p<0.01). Only four horses (three CB-affected and one healthy, p0.05) had detectable IgE levels against rAsp f 9. Furthermore, CB-affected horses were often sensitised against two or more r-allergens (13/26 of the CB-affected horses) while only one of the 24 healthy horses had positive IgE levels against more than one r-allergens. Similarly to IgE levels, no significant differences between CB-affected and healthy horses were found for IgG titres against the Aspergillus fumigatus extract. However, horses with CB had significantly higher serum IgG titres against rAsp f 8 than healthy controls (median=28 versus 10 relative ELISA units [REU], p<0.01). Additionally, horses with detectable IgE titres against rAsp f 8 had significantly higher IgG titres against this r-allergen than horses with undetectable IgE titres (median IgG titres=46 and 13 REU, respectively; p<0.01). For serum IgA titres, neither differences between healthy and CB-affected animals nor correlations between IgA and IgG or IgE titres could be found. These results show that horses suffering from CB are more often sensitised to some Aspergillus fumigatus and Alternaria alternata allergens than control horses and that they are partly sensitised to the same fungal proteins as mould-allergic human patients. Furthermore, this study shows that r-allergens allow a much more sensitive determination of specific serum antibody levels by ELISA than crude mould extracts.
Publication Date: 2000-03-14 PubMed ID: 10713338DOI: 10.1016/s0165-2427(00)00154-9Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Allergies
- Antibodies
- Aspergillus
- Bronchi
- Chronic Obstructive Pulmonary Disease
- Clinical Study
- Comparative Study
- Diagnosis
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Equine Health
- Horses
- Immunoglobulin A
- Immunoglobulin E
- Immunoglobulin G
- Immunology
- Inflammation
- Pulmonary Health
- Respiratory Disease
- Serum
- Veterinary Medicine
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research examined and compared the levels of specific antibodies in the blood of healthy horses and those suffering from Chronic Obstructive Pulmonary Disease (COPD). The findings revealed that COPD-affected horses show a higher sensitivity to certain types of fungal allergens.
Research Methodology
- A total of 24 healthy horses and 26 horses with Chronic Bronchitis/Bronchiolitis (CB), also known as COPD, were part of the study.
- The researchers used ELISA, a test that measures immune response in the blood, to determine levels of Immunoglobulin E antibody (IgE) against four recombinant (synthetic) mold allergens and crude (natural) mold and storage mite extracts.
- The four recombinant allergens were from molds called Aspergillus fumigatus and Alternaria alternata.
- The levels of IgG and IgA, types of antibodies in the sera, were also measured against Aspergillus fumigatus extract and one of its recombinant allergens.
Findings
- IgE could be measured against crude mold extracts in all sera (the component of blood that includes all proteins not used in blood clotting). However, there were no significant difference between these levels in CB-affected and control horses.
- Specific IgE levels against the synthetic allergens were detected only in 8-30% of the horses, varying according to the recombinant allergens used in the test.
- Horses with CB had significantly higher IgE levels than controls against certain recombinant allergens.
- CB-affected horses also showed sensitivity to two or more recombinant allergens, while only one healthy horse showed the same sensitivity.
- Similarly, no significant disparity was found in IgG levels against Aspergillus fumigatus extract between healthy and CB-affected horses.
- However, CB-affected horses showed higher IgG levels against a specific recombinant allergen (rAsp f 8) than healthy controls.
- No significant variation was observed in IgA levels between healthy and CB-affected horses.
- Recombinant allergens allowed for a more sensitive determination of specific serum antibody levels than crude mould extracts.
Conclusion
- The study shows that horses suffering from CB are often sensitized to certain allergens from Aspergillus fumigatus and Alternaria alternata molds more than control horses.
- They are also partly sensitised to the same fungal proteins as mold-allergic human patients, showing potential similarities in biological responses between species.
Cite This Article
APA
Eder C, Crameri R, Mayer C, Eicher R, Straub R, Gerber H, Lazary S, Marti E.
(2000).
Allergen-specific IgE levels against crude mould and storage mite extracts and recombinant mould allergens in sera from horses affected with chronic bronchitis.
Vet Immunol Immunopathol, 73(3-4), 241-253.
https://doi.org/10.1016/s0165-2427(00)00154-9 Publication
Researcher Affiliations
- Division of Immunogenetics, Institute of Animal Breeding, Bremgartenstrasse 109 A, 3012, Berne, Switzerland.
MeSH Terms
- Allergens / immunology
- Alternaria / immunology
- Animals
- Aspergillus fumigatus / immunology
- Enzyme-Linked Immunosorbent Assay / veterinary
- Female
- Fungal Proteins / immunology
- Horse Diseases / immunology
- Horse Diseases / microbiology
- Horses
- Immunoglobulin A / immunology
- Immunoglobulin E / analysis
- Immunoglobulin G / immunology
- Lung Diseases, Fungal / immunology
- Lung Diseases, Fungal / microbiology
- Lung Diseases, Fungal / veterinary
- Lung Diseases, Obstructive / immunology
- Lung Diseases, Obstructive / microbiology
- Lung Diseases, Obstructive / veterinary
- Male
- Mites / immunology
- Mitosporic Fungi / immunology
- Penicillium / immunology
- Recombinant Proteins / immunology
Citations
This article has been cited 18 times.- Wyler M, Sage SE, Marti E, White S, Gerber V. Protein microarray allergen profiling in bronchoalveolar lavage fluid and serum of horses with asthma.. J Vet Intern Med 2023 Jan;37(1):328-337.
- Intemann S, Reckels B, Schubert D, Wolf P, Kamphues J, Visscher C. The Hygienic Status of Different Forage Types for Horses-A Retrospective Study on Influencing Factors and Associations with Anamnestic Reports.. Vet Sci 2022 May 6;9(5).
- Di Pietro R, Dubuc V, Manguin E, Giroux-Lafond R, Bédard C, Boivin R, Lavoie JP, Vesper SJ, Leclere M. Characterization of fungal exposure and dectin-1 expression in healthy horses and horses with severe asthma.. Am J Vet Res 2022 May 8;83(6).
- Martins LML. Allergy to Fungi in Veterinary Medicine: Alternaria, Dermatophytes and Malassezia Pay the Bill!. J Fungi (Basel) 2022 Feb 27;8(3).
- Lo Feudo CM, Stucchi L, Alberti E, Conturba B, Zucca E, Ferrucci F. Intradermal Testing Results in Horses Affected by Mild-Moderate and Severe Equine Asthma.. Animals (Basel) 2021 Jul 13;11(7).
- White SJ, Moore-Colyer M, Marti E, Hannant D, Gerber V, Coüetil L, Richard EA, Alcocer M. Antigen array for serological diagnosis and novel allergen identification in severe equine asthma.. Sci Rep 2019 Oct 23;9(1):15170.
- White S, Moore-Colyer M, Marti E, Coüetil L, Hannant D, Richard EA, Alcocer M. Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma.. J Vet Intern Med 2019 Sep;33(5):2327-2335.
- Verdon M, Lanz S, Rhyner C, Gerber V, Marti E. Allergen-specific immunoglobulin E in sera of horses affected with insect bite hypersensitivity, severe equine asthma or both conditions.. J Vet Intern Med 2019 Jan;33(1):266-274.
- Einhorn L, Hofstetter G, Brandt S, Hainisch EK, Fukuda I, Kusano K, Scheynius A, Mittermann I, Resch-Marat Y, Vrtala S, Valenta R, Marti E, Rhyner C, Crameri R, Satoh R, Teshima R, Tanaka A, Sato H, Matsuda H, Pali-Schöll I, Jensen-Jarolim E. Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer.. Allergy 2018 Jul;73(7):1436-1446.
- Mueller RS, Janda J, Jensen-Jarolim E, Rhyner C, Marti E. Allergens in veterinary medicine.. Allergy 2016 Jan;71(1):27-35.
- Sarmiento J, Perez B, Morales N, Henriquez C, Vidal L, Folch H, Galecio JS, Morán G. Apoptotic effects of tamoxifen on leukocytes from horse peripheral blood and bronchoalveolar lavage fluid.. Vet Res Commun 2013 Dec;37(4):333-8.
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- Racine J, Gerber V, Feutz MM, Riley CP, Adamec J, Swinburne JE, Couetil LL. Comparison of genomic and proteomic data in recurrent airway obstruction affected horses using Ingenuity Pathway Analysis®.. BMC Vet Res 2011 Aug 15;7:48.
- Moran G, Buechner-Maxwell VA, Folch H, Henriquez C, Galecio JS, Perez B, Carrasco C, Barria M. Increased apoptosis of CD4 and CD8 T lymphocytes in the airways of horses with recurrent airway obstruction.. Vet Res Commun 2011 Oct;35(7):447-56.
- Morán G, Folch H, Araya O, Burgos R, Barria M. Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay.. Vet Res Commun 2010 Dec;34(8):719-26.
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- Swinburne JE, Bogle H, Klukowska-Rötzler J, Drögemüller M, Leeb T, Temperton E, Dolf G, Gerber V. A whole-genome scan for recurrent airway obstruction in Warmblood sport horses indicates two positional candidate regions.. Mamm Genome 2009 Aug;20(8):504-15.
- Langner KF, Jarvis DL, Nimtz M, Heselhaus JE, McHolland LE, Leibold W, Drolet BS. Identification, expression and characterisation of a major salivary allergen (Cul s 1) of the biting midge Culicoides sonorensis relevant for summer eczema in horses.. Int J Parasitol 2009 Jan;39(2):243-50.
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