Allergens of horse epithelium. I. Physicochemical and immunochemical characterization of five different horse epithelium raw materials used for allergen extract preparation.
Abstract: We investigated five horse epithelial allergen extracts prepared from different qualities of raw material by several biochemical and immunochemical methods. Horse serum albumin and horse serum were used to identify serum-related antigens. We found high similarities as well as marked differences between the extracts. There were strong differences in the protein contents, the protein patterns obtained by isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis, the total allergenic activities obtained by radioallergosorbent test inhibition assays and the amounts and numbers of serum-related proteins. The patterns of dander-related allergens in crossed radioimmunoelectrophoresis were similar but showed partly strong differences in allergen concentrations. Allergen compositions determined by blotting methods showed only minor differences between the investigated epithelial extracts. It appears that some of the differences, e.g. the content of serum-related proteins, depend on the way the different raw materials are prepared. Because serum-related extract components are not considered as major allergens, their content should be low in horse epithelial allergen extracts. For this reason whole skin cannot be recommended as starting material for horse epithelium allergen extract preparation.
Publication Date: 1990-01-01 PubMed ID: 2276848
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article investigates five different horse epithelial allergen extracts to examine variations in protein content, allergen activity and allergen compositions. The findings suggest different methods of raw material preparation can result in considerable variations in extract content, with implications on their allergenic potential.
Understanding Allergens and Extract Preparation
- Researchers analyzed allergen extracts derived from horse epithelium, the protective layer that covers most organs and cavities inside the body, including the skin, lungs, and digestive tract.
- The goal of the study was to determine possible differences between the extracts, which could be influenced by the type of raw material and the method of preparation used.
- The research included biochemical and immunochemical analyses to identify the types and levels of antigens (proteins which trigger immune responses) present in the extracts.
Findings and Differences
- The biochemical tests displayed strong differences in protein content and patterns between the extracts.
- Radioallergosorbent test (a blood test used to detect the presence of specific IgE antibodies) inhibition assays showed significant differences in allergenic activities.
- Researchers noted discrepancy in the amounts and variety of serum-related proteins, which are proteins found in the liquid portion of blood.
- Despite the recognized differences, allergen compositions, as identified by blotting methods, showed only minor differences between the horse epithelial extracts analyzed.
Implications of the Research
- The research suggests that differences in the preparation of raw materials can lead to considerable variations in the content of serum-related proteins in the allergen extracts.
- Since serum-related components are not considered major allergens, their presence should ideally be low in allergen extracts produced from horse epithelium, limiting allergenic potential.
- Based on their findings, the researchers propose that whole skin may not be an ideal raw material for the preparation of horse epithelium allergen extracts because of its potentially high serum-related protein content.
Cite This Article
APA
Franke D, Maasch HJ, Wahl R, Schultze-Werninghaus G, Bretting H.
(1990).
Allergens of horse epithelium. I. Physicochemical and immunochemical characterization of five different horse epithelium raw materials used for allergen extract preparation.
Int Arch Allergy Appl Immunol, 92(3), 309-317.
Publication
Researcher Affiliations
- Department of Research and Development of Allergen Extract Preparations, Allergopharma Joachim Ganzer KG, Reinbek b. Hamburg, FRG.
MeSH Terms
- Allergens / analysis
- Allergens / chemistry
- Allergens / isolation & purification
- Animals
- Blotting, Western
- Carbohydrates / analysis
- Chromatography, High Pressure Liquid
- Electrophoresis, Polyacrylamide Gel
- Epithelium / immunology
- Horses / immunology
- Immunoelectrophoresis
- Isoelectric Focusing
- Proteins / analysis
- Radioallergosorbent Test
Citations
This article has been cited 1 times.- Goubran Botros H, Gregoire C, Rabillon J, David B, Dandeu JP. Cross-antigenicity of horse serum albumin with dog and cat albumins: study of three short peptides with significant inhibitory activity towards specific human IgE and IgG antibodies. Immunology 1996 Jul;88(3):340-7.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
