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Home / Equine Research Bank / BMC Vet Res / Alterations in the glutathione metabolism could be implicate...
BMC veterinary research2009; 5; 10; doi: 10.1186/1746-6148-5-10

Alterations in the glutathione metabolism could be implicated in the ischemia-induced small intestinal cell damage in horses.

Abstract: Colic could be accompanied by changes in the morphology and physiology of organs and tissues, such as the intestine. This process might be, at least in part, due to the accumulation of oxidative damage induced by reactive oxygen (ROS) and reactive nitrogen species (RNS), secondary to intestinal ischemia. Glutathione (GSH), being the major intracellular thiol, provides protection against oxidative injury. The aim of this study was to investigate whether ischemia-induced intestinal injury could be related with alterations in GSH metabolism. Results: Ischemia induced a significant increase in lipid hydroperoxides, nitric oxide and carbon monoxide, and a reduction in reduced glutathione, and adenosine triphosphate (ATP) content, as well as in methionine-adenosyl-transferase and methyl-transferase activities. Conclusions: Our results suggest that ischemia induces harmful effects on equine small intestine, probably due to an increase in oxidative damage and proinflammatory molecules. This effect could be mediated, at least in part, by impairment in glutathione metabolism.
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Publication Date: 2009-03-18 PubMed ID: 19296836PubMed Central: PMC2664797DOI: 10.1186/1746-6148-5-10Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article explores the association between changes in glutathione metabolism and potential damage to small intestinal cells in horses due to ischemia, a condition often linked to colic in such animals.

Research Purpose and Methodology

  • The primary goal of the study was to examine whether damage to the intestinal tissue in horses resulting from ischemia is related to alterations in the metabolism of Glutathione (GSH), an important compound that protects against oxidative injury.
  • Ischemia is a condition wherein the blood flow to an organ is reduced, causing a shortage of oxygen and glucose needed for cellular metabolism, which leads to cell damage and dysfunction.
  • In this context, the researchers looked into a hypothetical scenario where ischemia-linked intestinal damage could be due to a build-up of oxidative harm caused by reactive oxygen (ROS) and reactive nitrogen species (RNS).

Investigation Outcomes

  • The researchers observed a significant increase in lipid hydroperoxides, nitric oxide, and carbon monoxide following ischemia, which could be indicators of increased oxidative stress.
  • Simultaneously, a reduction in reduced glutathione (an antioxidant form of GSH), adenosine triphosphate (ATP) content (required for energy in cells), and activities of enzymes like methionine-adenosyl-transferase and methyl-transferase were noted. Decreases in these components could contribute to cellular dysfunction.

Study Conclusion

  • The study concluded that ischemia has harmful effects on the small intestine of horses, potentially due to an increase in oxidative damage and pro-inflammatory molecules.
  • This harmful effect could be mediated in part by an impairment in glutathione metabolism. In other words, when glutathione metabolism gets impaired due to ischemia, it could lead to increased oxidative stress, causing further damage to the intestinal cells.

Cite This Article

APA
Marañón G, Manley W, Cayado P, García C, de la Muela MS, Vara E. (2009). Alterations in the glutathione metabolism could be implicated in the ischemia-induced small intestinal cell damage in horses. BMC Vet Res, 5, 10. https://doi.org/10.1186/1746-6148-5-10

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 5
Pages: 10

Researcher Affiliations

Marañón, Gonzalo
  • Horsepital SL, Villanueva del Pardillo, Madrid, Spain. gonzamara@yahoo.es
Manley, William
    Cayado, Patricia
      García, Cruz
        de la Muela, Mercedes Sánchez
          Vara, Elena

            MeSH Terms

            • Adenosine Triphosphate / metabolism
            • Animals
            • Carbon Monoxide / metabolism
            • Glutathione / metabolism
            • Horse Diseases / physiopathology
            • Horses / physiology
            • Intestine, Small / cytology
            • Intestine, Small / enzymology
            • Intestine, Small / metabolism
            • Intestine, Small / physiopathology
            • Ischemia / physiopathology
            • Ischemia / veterinary
            • Methionine Adenosyltransferase / metabolism
            • Methyltransferases / metabolism
            • Nitric Oxide / metabolism

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