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Journal of applied physiology (Bethesda, Md. : 1985)1989; 67(5); 2072-2077; doi: 10.1152/jappl.1989.67.5.2072

Altered sarcoplasmic reticulum function after high-intensity exercise.

Abstract: This study examined the effects of acute high-intensity exercise on the rate and capacity of Ca2+ uptake and Ca2+-stimulated adenosinetriphosphatase (ATPase) activity of the sarcoplasmic reticulum and the reversibility of these effects. Thoroughbred horses were run at maximal O2 uptake on a high-speed treadmill until fatigued. Muscle temperatures and biopsy samples were collected at rest, immediately after exercise, and 30 and 60 min after exercise. Blood samples were collected at rest and 5 min after exercise. Muscle and blood (lactate concentration) were three- and fivefold greater than pre-exercise values. Muscle temperature and pH immediately after post-exercise were 43 degrees C and 6.55, respectively, but approached rest values by 60 min after exercise. The initial rate and maximal capacity of Ca2+ uptake of muscle homogenates and isolated sarcoplasmic reticulum were significantly depressed immediately after exercise. This depression was paralleled by decreased activity of the Ca2+-stimulated ATPase. However, both Ca2+ uptake (rate and capacity) and Ca2+4-ATPase activity had returned to normal by 60 min after exercise. These findings demonstrate that changes in sarcoplasmic reticulum function after high-intensity exercise may be induced but not sustained by local changes in muscle pH and/or temperature.
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Publication Date: 1989-11-01 PubMed ID: 2532196DOI: 10.1152/jappl.1989.67.5.2072Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study on the impact of severe physical activity on a muscle’s capacity to control calcium levels and energy production, and how quickly it can recover these functions.

Research Objective

  • The purpose of this research was to investigate the effect of intense exercise on the capacity and speed of calcium uptake and the activity level of an enzyme, adenosinetriphosphatase (ATPase), in the sarcoplasmic reticulum of muscle cells. In addition, the research aimed to understand how quickly these functions could be restored post-exercise.

Methodology

  • Thoroughbred horses were used as subjects in the experiment. They were made to run on a high-speed treadmill at their maximum oxygen uptake level until they showed signs of fatigue.
  • Biopsy samples were collected at rest, immediately post-exercise, and 30 and 60 minutes after exercise. Blood samples were taken at rest and five minutes after exercise.
  • Temperature and pH levels in the horses’ muscles were documented. After exercise, muscle temperature reached 43 degrees C and pH level dropped to 6.55.
  • Blood lactate concentrations were determined as an indicator of exercise intensity.

Findings

  • Post-exercise, the amount of lactate in the blood and muscle was found to be three and five times the pre-exercise level respectively, indicating significant physical exertion.
  • Immediately after exercise, the capacity and initial rate of calcium uptake by the muscle cells and the isolated sarcoplasmic reticulum were significantly reduced. The activity level of the calcium-stimulated ATPase enzyme also decreased.
  • However, an hour after exercise, both the capacity and rate of calcium uptake and ATPase enzyme activity had returned to their pre-exercise levels.

Conclusion

  • The research concluded that changes in the functions of the sarcoplasmic reticulum, brought on by intense physical activity, could be caused by changes in the local temperature and pH level of the muscle, but these effects are temporary and the functions restore themselves within an hour of ceasing the exercise.

Cite This Article

APA
Byrd SK, McCutcheon LJ, Hodgson DR, Gollnick PD. (1989). Altered sarcoplasmic reticulum function after high-intensity exercise. J Appl Physiol (1985), 67(5), 2072-2077. https://doi.org/10.1152/jappl.1989.67.5.2072

Publication

Journal of applied physiology (Bethesda, Md. : 1985)
ISSN: 8750-7587
NlmUniqueID: 8502536
Country: United States
Language: English
Volume: 67
Issue: 5
Pages: 2072-2077

Researcher Affiliations

Byrd, S K
  • Department of Veterinary and Comparative Anatomy, College of Veterinary Medicine, Washington State University, Pullman 99164-6520.
McCutcheon, L J
    Hodgson, D R
      Gollnick, P D

        MeSH Terms

        • Analysis of Variance
        • Animals
        • Body Temperature
        • Ca(2+) Mg(2+)-ATPase / metabolism
        • Calcium / blood
        • Calcium / metabolism
        • Calcium-Transporting ATPases / metabolism
        • Female
        • Horses
        • Hydrogen-Ion Concentration
        • Male
        • Muscles / physiology
        • Oxygen Consumption / physiology
        • Physical Exertion / physiology
        • Sarcoplasmic Reticulum / physiology

        Grant Funding

        • 11H 8190 / PHS HHS

        Citations

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