Alternative solutions to hCG induction of ovulation in the mare.
Abstract: Injection of hCG (2000-2500 i.u., i.v.) to mares when a follicle reaches 35 mm induces ovulation between 24 and 48 h. However, repeated injections induce antibodies against hCG. We report attempts to induce ovulation without this inconvenience. We called 'response' an ovulation between 24 and 48 h after treatment. The typical response to hCG was obtained in 73% (N = 145) of treated mares. After immunization against hCG, the response (0%, N = 10) was less than in nonimmunized controls (100%, N = 9). Simultaneous injection of dexamethasone and hCG resulted in induction of ovulation (71%, N = 14). However, simultaneous hCG + dexamethasone resulted in antibody formation similar to that induced by hCG alone, when injected repeatedly every 21 days. Neither GnRH (2 mg i.m.) nor partly purified pig LH (26 mg i.v. or s.c.) induced ovulation consistently (40%, N = 30 and 31%, N = 16). Crude horse gonadotrophin (60 mg i.v. or s.c.) induced ovulation (86%, N = 14). Fertility was not different from control (61%, N = 13 vs 40%, N = 10). Crude horse gonadotrophin also induced ovulation in mares previously immunized against hCG (78%, N = 9): 50 or 25 mg gave satisfactory response (86%, N = 29 and 57%, N = 40). We conclude that crude horse gonadotrophin is a good alternative to hCG for the induction of ovulation in mares.
Publication Date: 1987-01-01 PubMed ID: 3479577
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- Journal Article
Summary
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The research article explores alternative solutions to the human chorionic gonadotrophin (hCG) for inducing ovulation in mares. It was found that crude horse gonadotrophin could be a useful substitute.
Objective of the Study
The study investigated alternatives to the use of hCG to induce ovulation in mares. The need for alternatives arises from the fact that multiple injections of hCG stimulate antibodies, hence making the hormone less effective.
Methods Employed
- The mares were injected with hCG when a follicle reached 35 mm, triggering ovulation between 24 to 48 hours.
- The standard response to hCG was realized in 73% of the treated mares.
- For mares immunized (a way to control hormone effect and behavior) against hCG, the response was completely abated, unlike the non-immunized control group which exhibited a 100% response rate.
- The effectiveness of combining dexamethasone (a type of steroid medication) with hCG was also studied.
- The researchers also explored other hormones such as GnRH (gonadotrophin-releasing hormone) and pig LH (luteinizing hormone).
- Furthermore, an examination of crude horse gonadotrophin was performed.
Findings from the Study
- Injection of both dexamethasone and hCG resulted in ovulation induction in 71% of the mares. However, the combination also resulted in the same level of antibodies against hCG as the hormone alone.
- Neither GnRH nor purified pig LH consistently induced ovulation, with only 40% and 31% effectiveness respectively.
- Most promisingly, crude horse gonadotrophin achieved an 86% success rate in inducing ovulation.
- Fertility results from crude horse gonadotrophin were comparable to the control group.
- Crude horse gonadotrophin was effective in inducing ovulation even in mares that had previously developed immunity against hCG, with satisfactory responses observed with doses as low as 25mg and 50mg.
Conclusion from the Study
The researchers concluded that crude horse gonadotrophin holds substantial potential as an alternative to hCG for inducing ovulation in mares, due to its efficacy even in hCG-immunized mares and fewer side effects.
Cite This Article
APA
Duchamp G, Bour B, Combarnous Y, Palmer E.
(1987).
Alternative solutions to hCG induction of ovulation in the mare.
J Reprod Fertil Suppl, 35, 221-228.
Publication
Researcher Affiliations
- INRA, Nouzilly, France.
MeSH Terms
- Animals
- Antibody Formation
- Chorionic Gonadotropin / immunology
- Chorionic Gonadotropin / pharmacology
- Dexamethasone / pharmacology
- Female
- Gonadotropins, Equine / pharmacology
- Horses / physiology
- Immunization / veterinary
- Luteinizing Hormone / pharmacology
- Ovulation Induction / veterinary
Citations
This article has been cited 12 times.- Derisoud E, Jouneau L, Dubois C, Archilla C, Jaszczyszyn Y, Legendre R, Daniel N, Peynot N, Dahirel M, Auclair-Ronzaud J, Wimel L, Duranthon V, Chavatte-Palmer P. Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass. BMC Genomics 2022 Jun 15;23(1):443.
- Bottrel M, Ortiz I, Hidalgo M, Díaz-Jiménez M, Pereira B, Consuegra C, Yousef MS, Dorado J. Hormonal Management for the Induction of Luteolysis and Ovulation in Andalusian Jennies: Effect on Reproductive Performance, Embryo Quality and Recovery Rate. Animals (Basel) 2022 Jan 8;12(2).
- Martinez de Andino EV, Brom-de-Luna JG, Canesin HS, Rader K, Resende HL, Ripley AM, Love CC, Hinrichs K. Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer. J Assist Reprod Genet 2019 Jun;36(6):1237-1250.
- Chang ZL, Li BX, Liu B, Yao L, Yu J, Jiang GM, Tan JH. Effects of FSH and the weather during induced ovulation and timed artificial insemination to increase jenny conception rates. Sci Rep 2019 Mar 1;9(1):3220.
- Clulow J, Clulow S, Guo J, French AJ, Mahony MJ, Archer M. Optimisation of an oviposition protocol employing human chorionic and pregnant mare serum gonadotropins in the barred frog Mixophyes fasciolatus (Myobatrachidae). Reprod Biol Endocrinol 2012 Aug 21;10:60.
- Fahiminiya S, Labas V, Roche S, Dacheux JL, Gérard N. Proteomic analysis of mare follicular fluid during late follicle development. Proteome Sci 2011 Sep 17;9:54.
- Mugnier S, Kervella M, Douet C, Canepa S, Pascal G, Deleuze S, Duchamp G, Monget P, Goudet G. The secretions of oviduct epithelial cells increase the equine in vitro fertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?. Reprod Biol Endocrinol 2009 Nov 19;7:129.
- Caillaud M, Duchamp G, Gérard N. In vivo effect of interleukin-1beta and interleukin-1RA on oocyte cytoplasmic maturation, ovulation, and early embryonic development in the mare. Reprod Biol Endocrinol 2005 Jun 22;3:26.
- Camillo F, Pacini M, Panzani D, Vannozzi I, Rota A, Aria G. Clinical use of twice daily injections of buserelin acetate to induce ovulation in the mare. Vet Res Commun 2004 Aug;28 Suppl 1:169-72.
- Dell'Aquila ME, Caillaud M, Maritato F, Martoriati A, Gérard N, Aiudi G, Minoia P, Goudet G. Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis. Reprod Biol Endocrinol 2004 Jun 22;2:44.
- Martoriati A, Gérard N. Interleukin-1 (IL-1) system gene expression in granulosa cells: kinetics during terminal preovulatory follicle maturation in the mare. Reprod Biol Endocrinol 2003 May 16;1:42.
- Gånheim A, Gånheim A, Jöchle W. Acceleration and timing of fertile ovulation in cyclic mares with a deslorelin implant. Acta Vet Scand 1995;36(4):393-400.
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