Amniotic membrane transplantation for corneal surface reconstruction after excision of corneolimbal squamous cell carcinomas in nine horses.
Abstract: The purpose of this study was to evaluate the usefulness and effectiveness of permanent amniotic membrane transplantation as an adjunctive treatment to superficial keratectomy alone or combined with strontium-90 irradiation for treatment of equine corneolimbal squamous cell carcinoma (SCC) to decrease corneal scarring and recurrence rate. Methods: The retrospective case study included 11 horses (n = 12 eyes) diagnosed and treated for ocular SCC that involved the limbus and cornea. Nine of those horses (n = 9 eyes) were treated between 2002 and 2006, with superficial lamellar keratectomy alone or combined with strontium-90 irradiation and followed by placement of a permanent amniotic membrane graft in the surgical defect. The level of scarring (i.e. the clarity of the cornea) resulting with the use of amniotic membrane was subjectively compared to cases where a permanent bulbar conjunctival graft was performed following keratectomy combined with strontium-90 irradiation or cryotherapy (n = 3 eyes). Recurrence was defined as the postoperative and postirradiation regrowth of SCC in the same site and globe. Results: The nine horses that received an amniotic membrane graft after keratectomy alone or combined with irradiation showed a minimal level of scarring in a cornea that regained a greater transparency in comparison to the horses that were treated with a bulbar conjunctival graft. All of the horses that received an amniotic membrane graft had 226 +/- 218 days of follow-up without tumor recurrence (mean +/- SD), ranging from 21 days to 778 days. Conclusions: The combination of superficial keratectomy alone or associated with beta-irradiation and permanent amniotic membrane transplantation is an effective treatment of corneal or corneolimbal SCC in horses. The placement of an amniotic membrane material represents an alternative surgical procedure to bulbar conjunctival grafts, especially if there is a lack of bulbar conjunctiva tissue available after tumor resection or if a particularly large corneal resection is necessary. The amniotic membrane is incorporated into the corneal defect and seems to create noticeably much less scarring than a corneal defect covered by bulbar conjunctiva.
Publication Date: 2006-11-02 PubMed ID: 17076873DOI: 10.1111/j.1463-5224.2006.00480.xGoogle Scholar: Lookup
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Summary
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The research article studies the effectiveness of using permanent amniotic membrane transplantation in conjunction with superficial keratectomy and strontium-90 irradiation for treating equine corneolimbal squamous cell carcinomas (SCC). The result indicated that amniotic membrane grafting led to lesser corneal scarring and prevented tumor recurrence, making it a favorable alternative to bulbar conjunctival grafts.
Introduction and Methodology
- The objective of the study was to assess whether permanent amniotic membrane transplantation could reduce corneal scarring and rate of recurrence after the removal of corneolimbal squamous cell carcinomas in horses.
- The study was a retrospective case study involving 12 eyes from 11 horses diagnosed and treated for ocular SCC affecting the limbus and cornea.
- Nine horses had their eyes treated between 2002 and 2006 using superficial lamellar keratectomy alone or along with strontium-90 irradiation, after which a permanent amniotic membrane graft was placed on the surgical defect.
- The researchers determined the level of scarring (the clarity of the cornea) by comparing it with cases where a bulbar conjunctival graft was performed after a similar surgical procedure combined with strontium-90 irradiation or cryotherapy.
- Tumor recurrence was defined as the regrowth of SCC in the same site and globe after surgery and irradiation.
Results
- Nine horses that received amniotic membrane grafts following keratectomy with or without irradiation showed minimal scarring, and their corneas regained more transparency compared to horses that were treated with bulbar conjunctival grafts.
- All horses that received amniotic membrane grafts showed no tumor recurrence for an average of 226 days after the procedure, with some horses showing no recurrence for up to 778 days.
- Therefore, the combination of superficial keratectomy plus beta-irradiation and permanent amniotic membrane transplantation was found to be an effective treatment for corneal or corneolimbal SCC in horses.
Conclusions
- The study concluded that amniotic membrane placement is a viable alternative surgical procedure to bulbar conjunctival grafts, especially when there’s a deficiency of bulbar conjunctiva tissue after tumor resection or if a large corneal resection is needed.
- The amniotic membrane is incorporated into the corneal defect and appears to cause significantly less scarring than a corneal defect covered by bulbar conjunctiva.
Cite This Article
APA
Ollivier FJ, Kallberg ME, Plummer CE, Barrie KP, O'Reilly S, Taylor DP, Gelatt KN, Brooks DE.
(2006).
Amniotic membrane transplantation for corneal surface reconstruction after excision of corneolimbal squamous cell carcinomas in nine horses.
Vet Ophthalmol, 9(6), 404-413.
https://doi.org/10.1111/j.1463-5224.2006.00480.x Publication
Researcher Affiliations
- Departments of Small Animal and Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610-0126, USA.
MeSH Terms
- Amnion / transplantation
- Animals
- Carcinoma, Squamous Cell / radiotherapy
- Carcinoma, Squamous Cell / surgery
- Carcinoma, Squamous Cell / veterinary
- Combined Modality Therapy
- Corneal Surgery, Laser
- Eye Neoplasms / radiotherapy
- Eye Neoplasms / surgery
- Eye Neoplasms / veterinary
- Female
- Florida / epidemiology
- Horse Diseases / epidemiology
- Horse Diseases / etiology
- Horse Diseases / pathology
- Horse Diseases / radiotherapy
- Horse Diseases / surgery
- Horses
- Limbus Corneae / surgery
- Male
- Radiotherapy, Adjuvant
- Retrospective Studies
- Treatment Outcome
Citations
This article has been cited 7 times.- Wells HC, Sizeland KH, Kirby N, Haverkamp RG. Structure and Strength of Bovine and Equine Amniotic Membrane. Biology (Basel) 2022 Jul 23;11(8).
- Lohajaroensub R, Sawangmake C, Rodkhum C, Tuntivanich N. Expression of Antimicrobial Peptide Genes in the Canine Amniotic Membrane. Vet Sci 2022 Apr 21;9(5).
- Withavatpongtorn N, Tuntivanich N. Characterization of Cryopreserved Canine Amniotic Membrane. Membranes (Basel) 2021 Oct 27;11(11).
- Lores M, Rakestraw P, De Rijck M, Yarbrough T. Use of autologous fascia lata graft to repair a complex corneal ulcer in a mare. Ir Vet J 2020;73:7.
- Kalpravidh M, Tuntivanich P, Vongsakul S, Sirivaidyapong S. Canine amniotic membrane transplantation for corneal reconstruction after the excision of dermoids in dogs. Vet Res Commun 2009 Dec;33(8):1003-12.
- Sandmeyer LS, Breaux CB, Grahn BH. Diagnostic ophthalmology. Squamous cell carcinoma. Can Vet J 2008 May;49(5):507-8.
- Selting KA, Schutte J, Sarol JN, Maitz CA, Morimoto C, Yee J, Najjar WA, Aldelaijan SI, Lattimer JC. Dosimetry of Continuous Random Motion in High Dose Rate Strontium-90 Plesiotherapy. Vet Radiol Ultrasound 2025 Jan;66(1):e70004.
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