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Reproduction in domestic animals = Zuchthygiene2020; 55(9); 1258-1262; doi: 10.1111/rda.13717

An approach to rescue the fertility of stallions with a high level of hemospermia.

Abstract: A high amount of blood and not the mere presence of blood in equine semen impacts fertility. The aim of this study was to develop an approach to rescue the fertility of stallions with high hemospermia levels. Semen from 15 stallions was divided into four experimental groups: (a) Control-pure raw semen, (b) WB50-50% (v/v) whole blood added into semen, (c) E1-WB50 extended in a 1:1 (v/v) ratio with milk-based extender and (d) E2-WB50 extended in a 2:1 ratio with milk-based extender. Sperm kinetics, plasma membrane integrity (PMI), lipid peroxidation (PER) and intracellular superoxide (O ) production were immediately evaluated. Four cycles of 20 mares were randomly assigned to the experimental groups. Mares were bred with an insemination dose of 1 billion total sperm and pregnancy was diagnosed 14 days after ovulation. Sperm kinetics could not be evaluated in the WB50 samples. Total motility was lower (p < .05) in E1 than in CT and E2 samples. Progressive motility decreased (p  .05); however, O production was higher (p  .05) for CT and E1 samples. The control (90%) and E2 (90%) groups had superior (p < .05) fertility than the others (WB50-0% and E1-25%). It was concluded that sperm motility and fertility of semen with a large amount of blood can be rescued by dilution with a 2:1 extender:semen ratio using a milk-based extender.
© 2020 Blackwell Verlag GmbH.
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Publication Date: 2020-08-10 PubMed ID: 32474982DOI: 10.1111/rda.13717Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores a new method to restore fertility in stallions that suffer from high levels of hemospermia, a condition characterized by the presence of blood in semen. The study suggests that diluting the semen with high blood content in a 2:1 ratio with a milk-based extender can improve both sperm motility and fertility.

Research Methodology

  • The study involved semen samples from 15 stallions, divided into four groups: Control (pure, raw semen), WB50 (50% whole blood added into semen), E1-WB50 (extended 1:1 with a milk-based extender), and E2-WB50 (extended 2:1 with milk-based extender).
  • The parameters tested in the semen samples included sperm kinetics, plasma membrane integrity (PMI), lipid peroxidation (PER), and intracellular superoxide production.

Experimental Contribution to Fertility

  • The research found that the sperm kinetics could not be evaluated in the WB50 samples. Total and progressive motility were lower in E1 compared to the Control and E2 samples.
  • Notably, the study shows that while PMI and PER did not significantly vary between the groups, the production of intracellular superoxide was noticeably higher in the WB50 group as compared to the E2 samples.
  • In terms of fertility, both the control and E2 groups fared superiorly, reaching a fertility rate of 90%, compared to the poor performance in fertility of the WB50 and E1 groups.

Conclusion

  • In conclusion, the study demonstrates that the fertility of semen with a high proportion of blood content can be improved by diluting it at a ratio of 2:1 using a milk-based extender. This is useful in treating stallions with high levels of hemospermia and in improving their fertility rates.

The research offers significant implications for the field of veterinary reproductive health, particularly around managing the fertility of stallions.

Cite This Article

APA
Andrade Junior LRP, Segabinazzi LGTM, Oliveira SN, Dell'Aqua JA, Papa FO. (2020). An approach to rescue the fertility of stallions with a high level of hemospermia. Reprod Domest Anim, 55(9), 1258-1262. https://doi.org/10.1111/rda.13717

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 55
Issue: 9
Pages: 1258-1262

Researcher Affiliations

Andrade Junior, Luiz Roberto P
  • School of Veterinary Medicine and Animal Science, Sao Paulo State University (UNESP), Botucatu, Brazil.
Segabinazzi, Lorenzo G T M
  • School of Veterinary Medicine and Animal Science, Sao Paulo State University (UNESP), Botucatu, Brazil.
Oliveira, Sidnei N
  • School of Veterinary Medicine and Animal Science, Sao Paulo State University (UNESP), Botucatu, Brazil.
Dell'Aqua, José Antonio
  • School of Veterinary Medicine and Animal Science, Sao Paulo State University (UNESP), Botucatu, Brazil.
Papa, Frederico O
  • School of Veterinary Medicine and Animal Science, Sao Paulo State University (UNESP), Botucatu, Brazil.

MeSH Terms

  • Animals
  • Cell Membrane
  • Female
  • Fertility
  • Hemospermia / veterinary
  • Horse Diseases
  • Horses
  • Insemination, Artificial / methods
  • Insemination, Artificial / veterinary
  • Lipid Peroxidation
  • Male
  • Pregnancy
  • Sperm Motility
  • Spermatozoa
  • Superoxides

References

This article includes 14 references
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Citations

This article has been cited 2 times.
  1. Segabinazzi LGTM, Podico G, Rosser MF, Nanjappa SG, Alvarenga MA, Canisso IF. Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma. Animals (Basel) 2021 May 21;11(6).
    doi: 10.3390/ani11061478pubmed: 34063777google scholar: lookup
  2. Egyptien S, Deleuze S, Ledeck J, Ponthier J. Sperm Quality Assessment in Stallions: How to Choose Relevant Assays to Answer Clinical Questions. Animals (Basel) 2023 Oct 6;13(19).
    doi: 10.3390/ani13193123pubmed: 37835729google scholar: lookup
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