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Home / Equine Research Bank / Can J Vet Res / An attempt to determine the tissue origin of equine serum al...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire1990; 54(1); 119-125;

An attempt to determine the tissue origin of equine serum alkaline phosphatase by isoelectric focusing.

Abstract: The main purpose of this study was to ascertain whether isoelectric point determination of alkaline phosphatase (AP) using an isoelectric focusing technique on agarose gels could define the isoenzymes present in healthy equine serum. The isoelectric points of AP extracted from nine tissues ranged from pH 3.5 to 7.5 with all tissues having multiple bands. There was considerable similarity in band pattern among tissues, with only pancreatic and colostral AP having substantially different isoelectric points from the others. Sera contained thirteen bands with isoelectric points ranging from pH 3.5 to 6.2 and as each band was common to more than one tissue it was not possible to define the tissue origin of these by direct comparison with tissue patterns. The intensity of all serum bands declined as foals aged, with the greatest decrease in bands 4 and 5 (numbered from the anode). There was no relative change in the banding pattern between early and late pregnant mares or in the sera of two foals before and after ingestion of colostrum. The mean (+/- SD) total serum AP activities of young foals (1676 +/- 1100 IU/L), three month foals (402 +/- 64 IU/L) early pregnant (190 +/- 54 IU/L) and late pregnant mares (109 +/- 26 IU/L) were significantly different from each other whereas colostral ingestion in two neonatal foals had no effect. We concluded that equine AP is a very heterogeneous protein and that normal horse sera do not contain significant renal or small intestinal derived AP. However isoelectric focusing alone could not differentiate bone from liver derived AP in sera.
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Publication Date: 1990-01-01 PubMed ID: 2306660PubMed Central: PMC1255615
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated the ability of isoelectric focusing techniques to identify the source tissue of alkaline phosphatase (AP) in horse serum, concluding that while the method can detect a wide variety of AP, it cannot distinguish between bone and liver derived AP.

Methodology

  • The research applied an isoelectric focusing technique on agarose gels, an approach typically used to separate proteins according to their isoelectric point (pI)—the pH at which a specific molecule carries no net electrical charge.
  • AP from nine different tissues was analysed for this study. For these tissue samples, the range of isoelectric points was found to be between pH 3.5 and 7.5.
  • Apart from pancreatic and colostral AP, all other tissues showed a remarkably similar band pattern, meaning that they had similar pI ranges.

Findings

  • Serum samples revealed thirteen bands with pI values ranging between pH 3.5 and 6.2. However, as each band was common to multiple tissues, it was not possible to specify the tissue origin of these bands based on a direct comparison with tissue patterns.
  • As foals aged, the intensity of all serum bands decreased, with bands 4 and 5 (numbered from the anode) demonstrating the most significant decrease.
  • Ingestion of colostrum and changes in mare pregnancy stages did not alter the banding pattern in serum samples.
  • A distinction in AP activities was observed within foals of different ages and pregnant mares in different stages. However, colostral intake by neonatal foals had no palpable effect.

Conclusion

  • The study proved that equine AP is an extremely diverse protein, therefore even with a sophisticated method like isoelectric focusing, it is a challenge to establish the tissue origin of the AP in horse serum.
  • The research also found that normal horse serum does not contain substantial levels of renal or small intestinal derived AP, which might shape the discussion for further studies.
  • However, the inability to differentiate bone from liver derived AP was a considerable limitation of the isoelectric focusing method in this context.

Cite This Article

APA
Ellison RS, Jacobs RM. (1990). An attempt to determine the tissue origin of equine serum alkaline phosphatase by isoelectric focusing. Can J Vet Res, 54(1), 119-125.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 0830-9000
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 54
Issue: 1
Pages: 119-125

Researcher Affiliations

Ellison, R S
  • Department of Pathology, Ontario Veterinary College, University of Guelph.
Jacobs, R M

    MeSH Terms

    • Alkaline Phosphatase / analysis
    • Alkaline Phosphatase / blood
    • Animals
    • Bone and Bones / enzymology
    • Colon / enzymology
    • Colostrum / enzymology
    • Female
    • Horses / metabolism
    • Isoelectric Focusing
    • Jejunum / enzymology
    • Kidney / enzymology
    • Liver / enzymology
    • Male
    • Neutrophils / enzymology
    • Pancreas / enzymology
    • Placenta / enzymology
    • Pregnancy

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