An ex vivo model for suture-less amniotic membrane transplantation with a chemically defined bioadhesive.
Abstract: Chemically defined bioadhesives (CDB) are "two-component reactive adhesives" made from antibiotic food additives. They contain no animal-derived ingredients and are characterized by a high level of self-degradability, low toxicity, and strong bonding properties. Amniotic membrane (AM) transplantation is used to repair severe ocular surface disease in humans and horses. AM requires meticulous suturing to the injured cornea. The purpose of this study is to determine whether CDB can facilitate the suture-less placement and adherence of equine AM to porcine cadaver cornea. Methods: Prospective study. Methods: To determine whether there are differences in ex vivo adhesion force between equine AM bonded with CDB to porcine cadaver cornea and equine AM sutured to porcine cadaver cornea. The following groups were tested (n = 5 per group): (1) CDB group: trephined (8 mm in diameter) cornea was bonded with CDB to 8-mm-sized discs of AM. (2) Suture group: the 8-mm trephined cornea was sutured with 8-0 absorbable suture to the AM. (3) Control group 1: two sheets of nitrocellulose paper were bonded with CDB. (4) Control group 2: two sheets of nitrocellulose paper were attached with suture and tested. Each sample was anchored to a tensiometer materials testing machine, and the strength of adhesion measured and expressed in units of kilogram-force (kgf). Results: Mean ± SD strength of adhesion force found no statistically significant difference between CDB group (0.091 ± 0.054 kgf; 15 min of CDB adhesion time) and suture groups (0.095 ± 0.037 kgf), whereas there was significant difference (P < 0.05) between control group 1 (0.679 ± 0.048 kgf) and control group 2 (0.585 ± 0.092 kgf). These results revealed that the 15 min of CDB adhesive time had adhesive properties as strong as suture group. Conclusions: CDB provides a satisfactory level of adherence to ex vivo suture-less attachment of equine amniotic membrane transplantation to the porcine cornea.
Publication Date: 2012-03-29 PubMed ID: 22458783DOI: 10.3109/02713683.2012.663853Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research attempts to explore whether Chemically Defined Bioadhesives (CDB) can facilitate the suture-less placement and adherence of equine Amniotic Membrane (AM) to a porcine cadaver cornea, a crucial procedure used to repair severe ocular surface disease.
Objective and Methodology
- The study is prospective in nature, aimed at determining if there are differences in adhesion force between equine AM bonded with CDB to porcine cadaver cornea and equine AM that is sutured to porcine cadaver cornea.
- The researchers divided the experiment into several groups: CDB group – where cornea was bonded with CDB to discs of AM, Suture group – where the cornea was sutured to the AM and two control groups where nitrocellulose paper was bonded with CDB or suture and tested.
Outcome Metrics
- To measure the efficacy, each sample was anchored to a tensiometer testing machine, where the strength of adhesion was measured in units of kilogram-force (kgf).
Results
- The findings of this research reveal no statistically significant difference between the adhesion strength of the CDB group and the suture groups. Thus, the 15 minutes of CDB adhesive time had adhesive properties as strong as the suture group.
- However, they found a significant difference in adhesion strength between the two control groups which used nitrocellulose paper instead of cornea and AM.
Conclusions
- Based on the outcomes, it’s concluded that CDB provides a satisfactory level of adherence for suture-less attachment of equine amniotic membrane transplantation to the porcine cornea.
- This could potentially change the approach to amniotic membrane transplantation, making it a suture-less procedure. Furthermore, it also highlights the potential of CDB as a useful adhesive in biological contexts.
Cite This Article
APA
Tsujita H, Brennan AB, Plummer CE, Nakajima N, Hyon SH, Barrie KP, Sapp B, Jackson D, Brooks DE.
(2012).
An ex vivo model for suture-less amniotic membrane transplantation with a chemically defined bioadhesive.
Curr Eye Res, 37(5), 372-380.
https://doi.org/10.3109/02713683.2012.663853 Publication
Researcher Affiliations
- Department of Small and Large Animal Sciences, Comparative Ophthalmology Service, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA. tsujitah@ufl.edu
MeSH Terms
- Adhesives / administration & dosage
- Adhesives / chemistry
- Amnion / transplantation
- Animals
- Cornea / pathology
- Cornea / surgery
- Corneal Ulcer / pathology
- Corneal Ulcer / surgery
- Disease Models, Animal
- Horses
- Prospective Studies
- Suture Techniques / instrumentation
- Sutures
- Swine
- Wound Healing
Citations
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