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An immunoperoxidase method applied to the diagnosis of equine herpesvirus abortion, using conventional and rapid microwave techniques.
Abstract: An indirect immunoperoxidase (IMP) technique was applied to cryostat and paraffin sections of liver from ten aborted equine foetuses. Equid herpesvirus type 1 (EHV-1) had been isolated from seven of them and EHV-4 from one: the remaining two were virologically negative and were not used as controls. In the eight virus-infected cases the immunostaining revealed foci of cells exhibiting a distinct brown cytoplasmic and inclusion body pigmentation. No specific signal was present in the non-infected controls. The method also was adapted for incubation in a microwave oven, which allowed the total length of the procedure to be reduced to a minimum of 35 mins for cryostat sections, without significant loss of signal.
Publication Date: 1992-01-01 PubMed ID: 1313357DOI: 10.1111/j.2042-3306.1992.tb02770.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article discusses the application of indirect immunoperoxidase (IMP) technique for diagnosing equine herpesvirus, leading to abortion, using both traditional and quick microwave methods. The IMP technique was used on tissue sections of liver obtained from ten aborted equine foetuses, revealing the presence of the virus in eight cases.
Indirect Immunoperoxidase Method
In the study, researchers turned to an indirect immunoperoxidase (IMP) technique to identify the presence of equine herpesvirus in the liver of aborted equine foetuses.
- IMP is an effective tool used in pathology to check for specific antigens (in this case EHV-1 and EHV-4) in tissues. The technique involves the application of an antibody which binds to the specific antigen in the tissue. This antibody is then targeted by a secondary, enzyme-linked antibody leading to a color reaction.
- The color staining aids in identifying the cells infected with the virus. In this research, infected cells showcased a distinct brown cytoplasmic and inclusion body pigmentation.
Cases and Controls Used in the Study
- The study used the livers of ten aborted equine foetuses for examination with the IMP technique. Out of these, seven had EHV-1 and one had EHV-4.
- The remaining two samples were virologically negative, indicating they were not infected with any equine herpesvirus. These samples were used as controls to compare with the infected samples, showing no specific signal.
Adaptability of the Method with Microwave Technique
The researchers also adapted the IMP method for use in a microwave oven to accelerate the process.
- The adaptation of the method allowed the total time of the procedure to be minimized to at least 35 minutes for cryostat sections, which are thin slices of tissue frozen for rapid diagnostic tests.
- This reduction in time did not result in any significant loss of the signal, hence not compromising the accuracy of the method.
Cite This Article
APA
Whitwell KE, Gower SM, Smith KC.
(1992).
An immunoperoxidase method applied to the diagnosis of equine herpesvirus abortion, using conventional and rapid microwave techniques.
Equine Vet J, 24(1), 10-12.
https://doi.org/10.1111/j.2042-3306.1992.tb02770.x Publication
Researcher Affiliations
- Department of Infectious Diseases, Animal Health Trust, Newmarket, Suffolk, UK.
MeSH Terms
- Abortion, Veterinary / diagnosis
- Animals
- Antigens, Viral / analysis
- Female
- Fluorescent Antibody Technique
- Frozen Sections
- Herpesviridae / immunology
- Herpesviridae / isolation & purification
- Herpesviridae Infections / diagnosis
- Herpesviridae Infections / veterinary
- Herpesvirus 1, Equid / immunology
- Herpesvirus 1, Equid / isolation & purification
- Horse Diseases / diagnosis
- Horses
- Immunoenzyme Techniques
- Liver / embryology
- Liver / microbiology
- Liver / pathology
- Microwaves
- Necrosis
- Paraffin Embedding
- Pregnancy
Grant Funding
- Wellcome Trust
Citations
This article has been cited 2 times.- Holz CL, Sledge DG, Kiupel M, Nelli RK, Goehring LS, Soboll Hussey G. Histopathologic Findings Following Experimental Equine Herpesvirus 1 Infection of Horses. Front Vet Sci 2019;6:59.
- Sutton GA, Viel L, Carman PS, Boag BL. Pathogenesis and clinical signs of equine herpesvirus-1 in experimentally infected ponies in vivo. Can J Vet Res 1998 Jan;62(1):49-55.
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