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Home / Equine Research Bank / Front Microbiol / An Oligosaccharide Rich Diet Increases Akkermansia spp. Bact...
Frontiers in microbiology2021; 12; 666039; doi: 10.3389/fmicb.2021.666039

An Oligosaccharide Rich Diet Increases Akkermansia spp. Bacteria in the Equine Microbiota.

Abstract: Some oligosaccharides induce growth of anti-inflammatory bacterial species and induce regulatory immunity in humans as well as animals. We have shown that the equine gut microbiota and the immune-microbial homeostasis largely stabilize within the first 50 days of life. Furthermore, we have previously established that certain bacterial species in the equine gut correlated with regulatory immunity. Accordingly, we hypothesized that an oligosaccharide rich diet fed to foals during the first 50 days would increase the abundance of bacterial species associated with regulatory immunity, and that this would influence immune responses in the foals. Eight pregnant mares and their foals were fed an oligosaccharide rich diet from 4 weeks before expected parturition until 49 days post-partum. Six mares and foals served as control. Fecal microbiota from mares and foals was characterized using 16S rRNA gene amplicon high throughput sequencing. On Day 49 the test foals had significantly higher abundances of Blood sampled from the foals in the test group on Day 7, 28, and 49 showed non-significant increases in IgA, and decreases in IgG on Day 49. In BALB/cBomTac mice inoculated with gut microbiota from test and control foals we found increased species richness, increased relative abundance of several species identified as potentially anti-inflammatory in horses, which were unclassified Clostridiales, Ruminococcaceae, , , and . We also found increased expression in the ileum if inoculated with test foal microbiota. We conclude that an oligosaccharide diet fed to foals in the "window of opportunity," the first 50 days of life, increases the abundance of anti-inflammatory species in the microbiota with potentially anti-inflammatory effects on regulatory immunity.
Copyright © 2021 Lindenberg, Lützhøft, Krych, Fielden, Kot, Frøkiær, van Galen, Nielsen and Hansen.
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Publication Date: 2021-05-21 PubMed ID: 34093482PubMed Central: PMC8176217DOI: 10.3389/fmicb.2021.666039Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research finds that feeding foals a diet rich in oligosaccharides – types of carbohydrate – in their first 50 days can lead to an increase in beneficial gut bacteria, which in turn fosters better immune regulation.

Objective of the Study

  • The main goal of this research was to observe the impact of an oligosaccharide-rich diet, when given to foals during their first 50 days, on the abundance of bacterial species associated with immune regulation.

Methods

  • Eight pregnant mares and their newborn foals were chosen for this study.
  • From 4 weeks prior to expected delivery until 49 days post-birth, they were fed a diet rich in oligosaccharides.
  • For comparison, six mares and foals were kept as control and insisted on a regular diet.
  • The fecal microbiota from both groups was characterized using 16S rRNA gene amplicon high throughput sequencing.

Results

  • On day 49, the foals in the test group showed significantly higher amounts of the bacterial species known as Akkermansia spp. which helps in various physiological processes including immune system regulation.
  • While blood samples from these foals did show increases in IgA levels, a marker of regulatory immune response, as well as decreases in IgG levels, these changes weren’t statistically significant.
  • The study also looked at outcomes in mice inoculated with gut microbiota from both test and control foals. These mice revealed an increased diversity of species and the expression of Akkermansia spp. when inoculated with the test foal microbiota.

Conclusion

  • The findings suggest that feeding foals an oligosaccharide-rich diet during their “window of opportunity,” or first 50 days of life, can lead to higher numbers of beneficial bacteria species, such as Akkermansia spp., in the gut.
  • These bacteria species could potentially have beneficial effects on the development of foals’ regulatory immune responses, which can impact their health and resistance to disease later in life.

Cite This Article

APA
Lindenberg FC, Lützhøft DO, Krych L, Fielden J, Kot W, Frøkiær H, van Galen G, Nielsen DS, Hansen AK. (2021). An Oligosaccharide Rich Diet Increases Akkermansia spp. Bacteria in the Equine Microbiota. Front Microbiol, 12, 666039. https://doi.org/10.3389/fmicb.2021.666039

Publication

Frontiers in microbiology
ISSN: 1664-302X
NlmUniqueID: 101548977
Country: Switzerland
Language: English
Volume: 12
Pages: 666039
PII: 666039

Researcher Affiliations

Lindenberg, Frederikke Christine
  • Brogaarden ApS, Lynge, Denmark.
  • Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Lützhøft, Ditte Olsen
  • Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Krych, Lukasz
  • Department of Food Sciences, Faculty of Sciences, University of Copenhagen, Copenhagen, Denmark.
Fielden, James
  • Brogaarden ApS, Lynge, Denmark.
Kot, Witold
  • Department of Environmental Sciences, Aarhus University, Aarhus, Denmark.
Frøkiær, Hanne
  • Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
van Galen, Gaby
  • Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Nielsen, Dennis Sandris
  • Department of Food Sciences, Faculty of Sciences, University of Copenhagen, Copenhagen, Denmark.
Hansen, Axel Kornerup
  • Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Conflict of Interest Statement

FL works at Brogaarden Diets. AKH declares that he has several industrial collaborations as further described on https://ivh.ku.dk/english/employees/?pure=en/persons/107126. The study received funding from Innovation Fund Denmark, who was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication. The study received funding from Brogaarden which is the employer of FL, and was therefore involved in the study design and execution.

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Citations

This article has been cited 9 times.
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