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Home / Equine Research Bank / Vet Sci / Analysis of 1840 Equine Intraocular Fluid Samples for the Pr...
Veterinary sciences2022; 9(8); doi: 10.3390/vetsci9080448

Analysis of 1840 Equine Intraocular Fluid Samples for the Presence of Anti-Leptospira Antibodies and Leptospiral DNA and the Correlation to Ophthalmologic Findings in Terms of Equine Recurrent Uveitis (ERU)-A Retrospective Study.

Abstract: In the equine clinic of the LMU in Munich, therapeutic vitrectomies have been routinely performed in horses for three decades. The vitreous samples obtained during vitrectomies were usually tested for anti-Leptospira antibodies and for more than 20 years also by PCR for leptospiral DNA. If the indication for surgery was ophthalmologically inconclusive, an aqueous humor was collected preoperatively and examined for evidence of leptospiral infection. In this study, medical records from 2002 to 2017 were analyzed. Records for 1387 eyes affected by equine recurrent uveitis (ERU) and 237 eyes affected by another type of uveitis met the inclusion criteria. A total of 216 samples from healthy eyes were used as controls. In 83% of intraocular samples from ERU eyes, antibody titers of 1:100 or higher were detectable by microscopic agglutination test (MAT). Similarly, 83% of intraocular samples had anti-Leptospira antibodies detected by ELISA. In 72% of the intraocular specimens, leptospiral DNA was detectable by PCR. No antibodies were detectable in the samples from eyes with another type of uveitis or in the samples from healthy eyes. A PCR was positive in only one sample from a healthy eye. These results with a very high number of intraocular specimens demonstrate the great importance of an intraocular leptospiral infection for ERU. It can be concluded that for a reliable diagnosis of intraocular leptospiral infection or to reliably exclude an infection multiple tests should be applied.
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Publication Date: 2022-08-21 PubMed ID: 36006363PubMed Central: PMC9414351DOI: 10.3390/vetsci9080448Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study conducted on horses spanning over a 15-year period (2002-2017), focusing on the correlation between intraocular Leptospira infection and equine recurrent uveitis (ERU). The study emphasizes the importance of multiple tests for a reliable diagnosis of intraocular Leptospira infection.

Research Methodology

  • The equine clinic of the LMU in Munich has processed therapeutic vitrectomies on horses for about 30 years. Vitreous samples, obtained during these procedures, traditionally underwent testing for anti-Leptospira antibodies and have been tested for the presence of Leptospira DNA via PCR for over 20 years.
  • In instances where the necessity for surgery was unclear based on ophthalmological findings, an aqueous humor was collected preoperatively and examined for signs of leptospiral infection.
  • The study leveraged medical records from 2002 to 2017 and used inclusion criteria to select 1387 eyes affected by ERU, 237 eyes impacted by other types of uveitis, and 216 samples from healthy eyes as controls.

Findings of the Study

  • A substantial proportion – 83%, of intraocular samples from ERU eyes displayed antibody titers of 1:100 or higher, detectable by the microscopic agglutination test (MAT). This was mirrored in the ELISA test results, where 83% of intraocular samples registered anti-Leptospira antibodies.
  • 72% of intraocular specimens showed the presence of leptospiral DNA as per PCR tests.
  • In contrast, no anti-Leptospira antibodies were detected in eyes affected by other types of uveitis or in samples collected from healthy eyes. An exception was one sample from a healthy eye which tested positive in PCR.

Research Conclusions

  • The study concluded that a substantial majority of ERU cases could potentially be linked to intraocular leptospiral infection according to a significant number of intraocular specimens.
  • The research recommends that for accurate diagnosis or reliable exclusion of intraocular leptospiral infection, practitioners should apply multiple testing methods.

Cite This Article

APA
Geiger T, Gerhards H, Bjelica B, Mackenthun E, Wollanke B. (2022). Analysis of 1840 Equine Intraocular Fluid Samples for the Presence of Anti-Leptospira Antibodies and Leptospiral DNA and the Correlation to Ophthalmologic Findings in Terms of Equine Recurrent Uveitis (ERU)-A Retrospective Study. Vet Sci, 9(8). https://doi.org/10.3390/vetsci9080448

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 9
Issue: 8

Researcher Affiliations

Geiger, Tobias
  • Clinic for Horses, University of Veterinary Medicine, 30559 Hanover, Germany.
Gerhards, Hartmut
  • Equine Clinic, Clinical Department, Ludwig-Maximilians-University (LMU), 85764 Oberschleissheim, Germany.
Bjelica, Bogdan
  • Department of Neurology, Hanover Medical School, 30625 Hanover, Germany.
Mackenthun, Elke
  • Equine Clinic at the Racetrack, An der Rennbahn 16, 76473 Iffezheim, Germany.
Wollanke, Bettina
  • Equine Clinic, Clinical Department, Ludwig-Maximilians-University (LMU), 85764 Oberschleissheim, Germany.

Conflict of Interest Statement

The authors declare no conflict of interest.

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