Analysis of mechanisms regulating the expression of parental alleles at the GPD locus in mule erythrocytes.
Abstract: Erythrocyte glucose-6-phosphate dehydrogenase (G6PD) was examined by 13% starch gel electrophoresis in 74 mules (42 females and 32 males), 35 donkeys, and ten horses. The quantitative expression of the parental alleles at the Gpd locus varies greatly in female mules from the hemizygous expression of the maternal allele to that of the paternal. The data obtained indicate that the X chromosomes are randomly inactivated in females mules. No selective advantage of a cell population with a maternally (or paternally) derived X active was found in female mule erythrocytes. It is suggested that the phenotypic variability in the expression of the parental Gpd alleles is related to the random proportions established between cells having either a maternal or paternal X active in an initiator (stem) cell group giving rise to erythroid tissue. Initiator cell numbers estimated for erythroid tissue (six or seven) are close to those reported for human females and intergeneric fox hybrids. These numbers may vary depending on the duration of the time of determination and the division rate of initiator cells at determination.
Publication Date: 1978-06-01 PubMed ID: 736879DOI: 10.1007/BF00484205Google Scholar: Lookup
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- Journal Article
Summary
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This research article investigates how genetic information from parent animals influences blood cell characteristics in mule offspring, with a particular focus on the enzyme glucose-6-phosphate dehydrogenase.
Objective of the Research
- This research aimed to examine the expression of parental genes at the Gpd (Glucose-6-phosphate dehydrogenase) locus in mule erythrocytes (red blood cells) and to discern the mechanisms that regulate this expression. The enzyme G6PD plays a crucial role in the red blood cells of many organisms, making it an appealing subject for study.
Sample and Methods Used in the Study
- The study analyzed G6PD in 74 mules (42 females and 32 males), 35 donkeys, and ten horses using 13% starch gel electrophoresis, a delicate technique used for separating proteins in an electric field.
Main Findings from the Research
- The researchers found that expression levels of parent-derived alleles at the Gpd locus vary significantly in female mules. This expression ranges from almost exclusively expressing the maternal genes to expressing genes inherited from the father.
- The data from the study indicate that the inactivation of X chromosomes occurs randomly in female mules. Essentially, X chromosome inactivation is a process by which one of the two copies of the X chromosome present in the cell of a female is inactivated to compensate for the double genetic dosage compared to males, who have just one X chromosome.
- The researchers found no evidence of selective advantage for a cell population with an active X chromosome derived from either parent.
Interpretation of the Research Findings
- The study suggests that the phenotypic variations (observable physical characteristics) in the expression of the parental Gpd alleles are linked to the random proportions between cells having either a maternal or paternal X active. This random proportion is established in an initiator or stem cell group, which gives rise to erythroid (red blood cell) tissue.
- The estimated numbers for initiator cell group for erythroid tissue (six or seven) are similar to those reported for human females and intergeneric fox hybrids. These numbers can fluctuate depending on the duration of the determination time and the division rate of the initiator cells at determination.
Cite This Article
APA
Serov OL, Zakijan SM, Kulichkov VA.
(1978).
Analysis of mechanisms regulating the expression of parental alleles at the GPD locus in mule erythrocytes.
Biochem Genet, 16(5-6), 379-386.
https://doi.org/10.1007/BF00484205 Publication
Researcher Affiliations
MeSH Terms
- Alleles
- Animals
- Electrophoresis, Starch Gel
- Erythrocytes / enzymology
- Female
- Glucosephosphate Dehydrogenase / genetics
- Horses / genetics
- Mosaicism
- Perissodactyla / genetics
- X Chromosome
References
This article includes 16 references
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- SHOWS TB Jr, TASHIAN RE, BREWER GJ, DERN RJ. ERYTHROCYTE GLUCOSE-6-PHOSPHATE DEHYDROGENASE IN CAUCASIANS: NEW INHERITED VARIANT.. Science 1964 Sep 4;145(3636):1056-7.
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- SANGER R, RACE RR, TIPPETT P, GAVIN J, HARDISTY RM, DUBOWITZ V. UNEXPLAINED INHERITANCE OF THE XG GROUPS IN TWO FAMILIES.. Lancet 1964 May 2;1(7340):955-6.
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Citations
This article has been cited 1 times.- Wang X, Miller DC, Clark AG, Antczak DF. Random X inactivation in the mule and horse placenta. Genome Res 2012 Oct;22(10):1855-63.
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