Analysis of non-porcine isolates of Actinobacillus suis.
Abstract: Twenty-four Actinobacillus suis isolates obtained from several species of non-porcine mammals were compared to the representative porcine strains, ATCC 15557 (serotype O1) and H89-1173 (serotype O2), by O serotyping, DNA fingerprinting, PCR amplification of apxICA, apxIICA and apxIIICA toxin genes and by rrs (16S rRNA) gene sequencing. Only two strains, both equine, reacted with O1 antiserum while two others, one canine and the other feline, reacted with O2 antiserum. One equine strain reacted weakly with both antisera. No amplification of apx genes was found with the non-porcine O1 or the "not O1/O2" strains but amplification of the apxICA and apxIICA genes was observed with the two O2 strains. In addition, these two O2 strains had both BamHI and BglII fingerprints that were very similar to the porcine O2 reference strain, H89-1173 and rrs gene sequences that were identical to the A. suis reference strain ATCC 15557. Taken together, these data suggest that although many non-porcine A. suis isolates are not A. suis (sensu stricto), some isolates are genotypically as well as phenotypically similar to A. suis.
Publication Date: 2002-01-17 PubMed ID: 11792495DOI: 10.1016/s0378-1135(01)00484-9Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research attempts to analyze and compare the characteristics of Actinobacillus suis bacteria isolated from several non-pig species with representative pig strains. After conducting numerous tests, the study finds that while most of the non-pig isolates do not strongly resemble the pig strains, a select few share significant genetic and phenotypic similarities with them.
Objective of the Research
- The main objective of this research was to compare 24 distinct Actinobacillus suis bacteria strains isolated from various non-porcine mammals to two representative strains found in pigs, to study their similarities and differences.
Methodology
- The comparison was carried out through multiple methods: O serotyping (a test to identify bacteria strains based on the type of their coating), DNA fingerprinting, PCR amplification of three types of apx toxin genes, and sequencing of the rrs (16S rRNA) gene.
- O1 and O2 antisera were used to see if the non-porcine strains react similarly to the pig strains.
Results
- Of all the non-porcine strains tested, only two strains from horses showed a reaction with the O1 antiserum. One strain each from dog and cat reacted with O2 antiserum.
- No amplification of apx genes was seen in the non-porcine strains that reacted with O1 antiserum or those that did not react with either O1 or O2 antisera.
- However, strains that reacted with O2 antiserum did show amplification of apxICA and apxIICA genes.
- The non-porcine isolates that reacted with O2 antiserum also showed similar DNA fingerprints as the porcine O2 reference strain when treated with restriction enzymes BamHI and BglII.
- The same strains also had identical rrs gene sequences to the porcine A. suis reference strain.
Conclusion
- The data suggests that while many of the non-porcine isolates of A.suis bear significant genetic differences from porcine isolates, a few strains, especially those that react with O2 antiserum, show considerable genetic and physical resemblance to the pig strains.
- This could indicate that the bacteria strains in these non-porcine animals are the same species as those found in pigs, or that the bacteria has crossed over among species.
Cite This Article
APA
Jeannotte ME, Slavić D, Frey J, Kuhnert P, MacInnes JI.
(2002).
Analysis of non-porcine isolates of Actinobacillus suis.
Vet Microbiol, 85(1), 83-93.
https://doi.org/10.1016/s0378-1135(01)00484-9 Publication
Researcher Affiliations
- Département de Pathologie et de Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, Saint-Hyacinthe, Qué., Canada J2S 7C6.
MeSH Terms
- Actinobacillus / classification
- Actinobacillus / genetics
- Actinobacillus Infections / microbiology
- Actinobacillus Infections / veterinary
- Animals
- Bacterial Proteins / genetics
- Bacterial Toxins / genetics
- Cats
- DNA Fingerprinting / methods
- DNA Fingerprinting / veterinary
- Dogs
- Gene Amplification
- Genes, Bacterial
- Genotype
- Hemolysin Proteins
- Horses
- Phenotype
- Polymerase Chain Reaction / methods
- Polymerase Chain Reaction / veterinary
- Rabbits
- Serotyping / methods
- Serotyping / veterinary
- Species Specificity
- Swine
Citations
This article has been cited 3 times.- Ishihara M, Yamazaki Y, Katsuda K, Ito H. Phenotypic and genotypic characterization of Actinobacillus suis sensu stricto isolated from a dairy calf. J Vet Med Sci 2022 May 1;84(5):624-627.
- Bujold AR, MacInnes JI. Identification of putative adhesins of Actinobacillus suis and their homologues in other members of the family Pasteurellaceae. BMC Res Notes 2015 Nov 14;8:675.
- Ojha S, Sirois M, Macinnes JI. Identification of Actinobacillus suis genes essential for the colonization of the upper respiratory tract of swine. Infect Immun 2005 Oct;73(10):7032-9.
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