Analysis of proglycogen and macroglycogen content in muscle biopsy specimens obtained from horses.
Abstract: To determine proglycogen (PG) and macroglycogen (MG) content in equine skeletal muscle and to compare 2 analytical methods (acid hydrolysis [AC] and PG plus MG determination) for measurement of total muscle glycogen content (Gly(tot)) in biopsy specimens. Methods: Muscle biopsy specimens obtained from 41 clinically normal horses. Methods: Forty-five muscle biopsy specimens obtained from the middle gluteal (n = 31) or triceps (14) muscle were analyzed, using AC and MG plus PG determination for Gly(tot). Variability within muscle biopsy specimens for each method was calculated from duplicate analyses of muscle specimens. In a second experiment, variation in MG and PG content between muscle biopsy specimens and the effect of sample collection depth on the concentration of MG and PG in the middle gluteal muscle was evaluated. Results: There was a strong correlation (r = 0.99) between Gly(tot) values obtained by use of AC and MG plus PG determination. Coefficients of variation for within- and between-specimen variability of Gly(tot) were approximately 4% for each method. The PG fraction was always in excess of the MG fraction. Biopsy specimens obtained from the superficial part of the middle gluteal muscle contained significantly more Gly(tot) and PG than specimens obtained from deeper parts. Conclusions: This study confirms that MG and PG exist in equine skeletal muscle and can be measured reliably in biopsy samples. This technique could be applied in future studies to investigate glycogen metabolism in exercising horses and horses with glycogen-storage diseases.
Publication Date: 2002-04-10 PubMed ID: 11939321DOI: 10.2460/ajvr.2002.63.570Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research aimed to measure the concentration of proglycogen and macroglycogen in horse muscle tissue and compared two analytical methods used for this purpose. It found that both kinds of glycogen exist in equine muscle tissue and can be reliably measured using biopsy samples.
Research Methodologies
The research utilised two main methodologies:
- First, a muscle biopsy was obtained from 41 clinically normal horses. These samples were collected from either the middle gluteal or triceps muscle and used to examine the concentration of proglycogen (PG) and macroglycogen (MG) in horse muscle tissue.
- Second, the researchers directly compared two analytical methods for measuring total muscle glycogen content – acid hydrolysis (AC) and PG plus MG determination.
- In a parallel experiment, the variation in MG and PG content between muscle biopsy specimens and the influence of sample collection depth were evaluated.
Findings
Some of the key findings of the study include:
- A strong correlation (r=0.99) was found between total muscle glycogen values attained using the AC method and the PG plus MG determination method.
- Both methods exhibited comparable coefficients of variation of about 4% for within and between-specimen variability of total muscle glycogen content.
- The PG fraction was consistently larger than the MG fraction.
- The quantity of total muscle glycogen and PG was significantly higher in biopsy samples taken from the superficial part of the middle gluteal muscle, as opposed to specimens obtained from deeper muscle parts.
Conclusions
Through this study, the researchers reached these conclusions:
- Both macroglycogen and proglycogen exist in horse skeletal muscles and can be reliably measured using biopsy samples.
- This method could potentially be applied in future studies to explore glycogen metabolism in exercising horses or horses suffering from glycogen-storage diseases.
Essentially, this work forms a functional basis upon which future research related to glycogen metabolism in horses may be carried out.
Cite This Article
APA
Bröjer JT, Stämpfli HR, Graham TE.
(2002).
Analysis of proglycogen and macroglycogen content in muscle biopsy specimens obtained from horses.
Am J Vet Res, 63(4), 570-575.
https://doi.org/10.2460/ajvr.2002.63.570 Publication
Researcher Affiliations
- Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Canada.
MeSH Terms
- Animals
- Biopsy, Needle / methods
- Biopsy, Needle / veterinary
- Female
- Glycogen / analysis
- Glycogen / metabolism
- Horses / metabolism
- Hydrolysis
- Male
- Muscle, Skeletal / chemistry
- Muscle, Skeletal / metabolism
- Perchlorates / chemistry
- Regression Analysis
- Reproducibility of Results
Citations
This article has been cited 3 times.- Marchand I, Tarnopolsky M, Adamo KB, Bourgeois JM, Chorneyko K, Graham TE. Quantitative assessment of human muscle glycogen granules size and number in subcellular locations during recovery from prolonged exercise.. J Physiol 2007 Apr 15;580(Pt. 2):617-28.
- Gondim FJ, Modolo LV, Campos GE, Salgado I. Neuronal nitric oxide synthase is heterogeneously distributed in equine myofibers and highly expressed in endurance trained horses.. Can J Vet Res 2005 Jan;69(1):46-52.
- Bröjer JT, Stämpfli HR, Graham TE. Effect of extraction time and acid concentration on the separation of proglycogen and macroglycogen in horse muscle samples.. Can J Vet Res 2002 Jul;66(3):201-6.
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