Analysis of soluble CD14 and its use as a biomarker in neonatal foals with septicemia and horses with recurrent airway obstruction.
Abstract: Soluble CD14 (sCD14) binds bacterial lipopolysaccharide (LPS) and acts as an anti-inflammatory LPS-inhibitor in vivo. In humans, sCD14 is one of the soluble biomarkers used for various inflammatory diseases and conditions, however, sCD14 assays have not yet been evaluated in horses. Here, we developed and optimized a bead-based assay for the quantification of sCD14 in horses. The assay was then used to determine native sCD14 concentrations in serum from healthy and septic foals, in the colostrum of healthy mares and in plasma from adult horses with recurrent airway obstruction (RAO) and control horses. Healthy foals and adult horses had sCD14 concentrations in serum or plasma in the high ng/ml range. The concentration of sCD14 in colostrum samples from healthy mares was in the μg/ml range. Foals with septicemia and adult horses with RAO had significantly higher sCD14 concentrations in their circulation than the respective control groups. The findings suggest that sCD14 can become a valuable biomarker for neonatal septicemia, RAO and possibly also for other inflammatory diseases in horses. Further studies and larger samples numbers are required to determine normal sCD14 concentration ranges and those that are indicative of disease progression, severity or prognosis.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication Date: 2013-06-06 PubMed ID: 23810420DOI: 10.1016/j.vetimm.2013.05.018Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article is about a study that developed a bead-based assay for measuring soluble CD14 (sCD14) in horses and investigates its potential as a biomarker for detecting neonatal septicemia and recurrent airway obstruction (RAO).
Methodology and Assay Development
- The researchers created and optimised a bead-based assay for measuring sCD14, a substance that detects bacterial infection and acts as an anti-inflammatory agent in horses. Prior to this study, this assay had not been used for horse-related research.
- The developed bead-based assay was used to measure sCD14 concentrations in various samples derived from horses, including the serum of healthy and septic foals, the colostrum (first form of milk immediately following delivery) of healthy mares, and the plasma of adult horses suffering from recurrent airway obstruction (RAO) and a control group of healthy adult horses.
Measurement Findings
- The research illustrated that healthy foals and adult horses typically presented sCD14 concentrations in the high ng/ml range in their serum or plasma.
- The colostrum of healthy mares manifested sCD14 concentrations in the μg/ml range, indicating a higher concentration level than in the serum or plasma of healthy foals and adult horses.
- Foals affected by septicemia (a severe bloodstream infection) and adult horses with RAO presented significantly higher concentrations of sCD14 in their circulation than the respective healthy control groups.
Conclusion and Future Research
- Based on the findings, the researchers proposed that sCD14 could serve as a valuable biomarker for diagnosing neonatal septicemia and recurrent airway obstruction in horses, and possibly for other inflammatory diseases as well.
- The study acknowledges that further research is needed. Larger samples will need to be collected in order to establish the ranges for normal sCD14 concentration, and those indications of disease progression, severity and prognosis.
Cite This Article
APA
Wagner B, Ainsworth DM, Freer H.
(2013).
Analysis of soluble CD14 and its use as a biomarker in neonatal foals with septicemia and horses with recurrent airway obstruction.
Vet Immunol Immunopathol, 155(1-2), 124-128.
https://doi.org/10.1016/j.vetimm.2013.05.018 Publication
Researcher Affiliations
- Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. bw73@cornell.edu
MeSH Terms
- Airway Obstruction / blood
- Airway Obstruction / immunology
- Airway Obstruction / veterinary
- Animals
- Animals, Newborn
- Antibodies, Monoclonal
- Biomarkers / blood
- Case-Control Studies
- Colostrum / immunology
- Female
- Fluoroimmunoassay / methods
- Fluoroimmunoassay / veterinary
- Horse Diseases / blood
- Horse Diseases / immunology
- Horses / blood
- Horses / immunology
- Immunity, Maternally-Acquired
- Lipopolysaccharide Receptors / blood
- Pregnancy
- Recurrence
- Reference Values
- Sepsis / blood
- Sepsis / immunology
- Sepsis / veterinary
- Solubility
Citations
This article has been cited 7 times.- Hagen A, Lehmann H, Aurich S, Bauer N, Melzer M, Moellerberndt J, Patané V, Schnabel CL, Burk J. Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture.. Front Bioeng Biotechnol 2020;8:613621.
- Taylor S. A review of equine sepsis.. Equine Vet Educ 2015 Feb;27(2):99-109.
- Schnabel CL, Babasyan S, Rollins A, Freer H, Wimer CL, Perkins GA, Raza F, Osterrieder N, Wagner B. An Equine Herpesvirus Type 1 (EHV-1) Ab4 Open Reading Frame 2 Deletion Mutant Provides Immunity and Protection from EHV-1 Infection and Disease.. J Virol 2019 Nov 15;93(22).
- Sheats MK. A Comparative Review of Equine SIRS, Sepsis, and Neutrophils.. Front Vet Sci 2019;6:69.
- Wimer CL, Schnabel CL, Perkins G, Babasyan S, Freer H, Stout AE, Rollins A, Osterrieder N, Goodman LB, Glaser A, Wagner B. The deletion of the ORF1 and ORF71 genes reduces virulence of the neuropathogenic EHV-1 strain Ab4 without compromising host immunity in horses.. PLoS One 2018;13(11):e0206679.
- Schnabel CL, Wimer CL, Perkins G, Babasyan S, Freer H, Watts C, Rollins A, Osterrieder N, Wagner B. Deletion of the ORF2 gene of the neuropathogenic equine herpesvirus type 1 strain Ab4 reduces virulence while maintaining strong immunogenicity.. BMC Vet Res 2018 Aug 22;14(1):245.
- Ortved K, Wagner B, Calcedo R, Wilson J, Schaefer D, Nixon A. Humoral and cell-mediated immune response, and growth factor synthesis after direct intraarticular injection of rAAV2-IGF-I and rAAV5-IGF-I in the equine middle carpal joint.. Hum Gene Ther 2015 Mar;26(3):161-71.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
