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Reproduction (Cambridge, England)2002; 124(2); 241-248; doi: 10.1530/rep.0.1240241

Analysis of the variations of follicular fluid composition during follicular growth and maturation in the mare using proton nuclear magnetic resonance (1H NMR).

Abstract: Follicular development and ovulatory processes in mammals involve local biochemical changes as a result of substantial modifications in cellular metabolism, the most well known of which is steroid variation. In the present study, the intrafollicular variation of several other components was studied using proton nuclear magnetic resonance ((1)H NMR). This approach made it possible to demonstrate that the intrafollicular biochemical content changes during follicular growth and maturation. Follicular fluid was aspirated by ovarian puncture of the dominant follicle at various physiological stages of its development: early dominant, late dominant and preovulatory. Serum samples were collected during each puncture session. (1)H NMR was used to evaluate intrafollicular and circulating glycoconjugates (sugar chains and N-acetyl groups), lipoproteins (CH(3) and CH(2) groups), glucose metabolites (trimethylamines, acetate and lactate), amino acids (glutamine/glutamate and alanine), creatine/creatinine and polyamines. Follicular fluids were assayed by radioimmunoassay for oestradiol and progesterone contents. The intrafollicular contents of alanine and lipoproteins (CH(3) groups) decreased in the dominant follicle during growth, whereas concentrations of progesterone and oestradiol increased significantly. After injection of gonadotrophin to induce ovulation, follicular maturation was characterized by a decrease in glycoconjugates (sugar chains), trimethylamines and acetate, a decrease in oestradiol concentration, and a further increase in CH(3) groups of lipoproteins and progesterone. The results from the present study showed a clear correlation between the intrafollicular content of alanine and that of oestradiol. A correlation between progesterone and glycoconjugates (sugar chains) was also observed. Therefore, (1)H NMR was shown to be effective for studying specific changes in the biochemical composition of the follicular fluid that occur during follicular development. For the first time, the variation of several compounds (glycoconjugates, lipoproteins, glucose metabolites, amino acids and polyamines) in relation to growth and maturation was demonstrated. Some of these changes could be of crucial importance for follicular maturation and ovulation as well as for oocyte maturation and further fertilization.
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Publication Date: 2002-07-27 PubMed ID: 12141937DOI: 10.1530/rep.0.1240241Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

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The study investigates the changes in the biochemical composition of the follicular fluid during follicle development and maturation in mares using proton nuclear magnetic resonance (1H NMR). It shows for the first time how multiple compounds vary in relation to this growth and maturation, some of which may be critical for maturation and ovulation of the follicle, and ultimately, fertilization of the oocyte.

Methodology

  • The researchers focused on follicle development and ovulatory processes in mammals which involve local biochemical changes due to cellular metabolism modifications. The variations of several components were studied using (1)H NMR.
  • Follicular fluid was extracted through ovarian puncture at various physiological stages: early dominant, late dominant and preovulatory. At the same time, serum samples were collected.
  • The (1)H NMR was subsequently used to assess the intrafollicular and circulating levels of glycoconjugates, lipoproteins, glucose metabolites, amino acids, creatine/creatinine, and polyamines within the fluid.
  • The follicular fluids were also tested for oestradiol and progesterone contents through a radioimmunoassay.

Findings

  • The dominant follicle saw a decrease in the intrafollicular content of alanine and lipoproteins during growth, whereas the concentrations of progesterone and oestradiol significantly increased.
  • After inducing ovulation by injecting gonadotrophin, follicular maturation was characterized by a decrease in glycoconjugates, trimethylamines and acetate, decline in oestradiol concentration, and a further increase in the lipoprotein’s CH(3) groups and progesterone.
  • The results showed a clear correlation between the intrafollicular content of alanine and oestradiol. A correlation between progesterone and glycoconjugates was also seen.

Conclusions

  • This study signals that (1)H NMR can effectively identify specific changes in the biochemical composition of follicular fluid during follicular development.
  • For the first time, this study demonstrated variation in several compounds (glycoconjugates, lipoproteins, glucose metabolites, amino acids, and polyamines) related to follicular growth and maturation.
  • This research indicates that some of these variations could play an important role in follicular maturation and ovulation as well as the maturation of the oocyte and possible fertilization outcome.

Cite This Article

APA
Gérard N, Loiseau S, Duchamp G, Seguin F. (2002). Analysis of the variations of follicular fluid composition during follicular growth and maturation in the mare using proton nuclear magnetic resonance (1H NMR). Reproduction, 124(2), 241-248. https://doi.org/10.1530/rep.0.1240241

Publication

Reproduction (Cambridge, England)
ISSN: 1470-1626
NlmUniqueID: 100966036
Country: England
Language: English
Volume: 124
Issue: 2
Pages: 241-248

Researcher Affiliations

Gérard, N
  • Equipe Reproduction Equine, PRC, INRA-Haras Nationaux, F-37380 Nouzilly, France. gerard@tours.inra.fr
Loiseau, S
    Duchamp, G
      Seguin, F

        MeSH Terms

        • Alanine / metabolism
        • Animals
        • Estradiol / metabolism
        • Female
        • Follicular Fluid / metabolism
        • Glucose / metabolism
        • Glycoconjugates / metabolism
        • Horses / metabolism
        • Lipoproteins / metabolism
        • Magnetic Resonance Spectroscopy
        • Ovarian Follicle / growth & development
        • Polyamines / metabolism
        • Progesterone / metabolism

        Citations

        This article has been cited 21 times.
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