Anti-collagen antibodies and immune complexes in equine joint diseases.
Abstract: An investigation was made into the possible contribution of autoimmune mechanisms to equine arthropathies. Serum and synovial fluid (SF) immune complexes and anti-collagen Type II antibodies were measured, by ELISA, in groups of horses with naturally occurring osteoarthritis (OA), osteochondritis dissecans (OCD), bone fracture, traumatised joints, synovitis, infected joints and non-diseased (control) joints. Significantly raised anti-collagen Type II antibodies were found in osteoarthritic (P < 0.02) and traumatised joint synovial fluids (P < 0.01) compared with the control, where ten of 38 (26%) OA and five of eight (63%) traumatised joint synovial fluid samples had raised anti-collagen Type II antibodies (above mean and 2 SD of control group; P < 0.05). Significantly raised levels of C1q-binding immune complexes were found in the synovial fluids of horses with OA (P < 0.001), OCD (P < 0.02), fractured articular bone (P < 0.001), infected (P < 0.01) and traumatised joints (P < 0.001) as compared with the control horses. Raised C1q-binding activity (above mean and 2 SD of control group; P < 0.05) was shown in synovial fluids in 18 of 38 (47%) OA, four of 16 (25%) OCD, four of nine (44%) fractured bone, one of eight (14%) synovitis, two of four (50%) infected joints and seven of eight (88%) traumatised joints. No raised anti-collagen Type II antibodies or C1q-binding activity were detected in sera from any clinical group. Correlations were found, in synovial fluids, between anti-collagen Type II antibodies and C1q-binding activity in OA (P < 0.02), synovitis (P < 0.01) and infected joint (P < 0.05) groups. Within individual horses, there were no correlations between the SF and sera measurements of either anti-collagen II or C1q-binding activity. The relationship between cause and effect of these immunological findings cannot be determined; as they are common to many types of equine joint disorder it is probable that they are not an initiating factor in pathology.
Publication Date: 1995-03-01 PubMed ID: 7604535DOI: 10.1016/0165-2427(94)05325-mGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper presents an investigation on the role of autoimmune mechanisms in horse joint diseases. It outlines the detection of anti-collagen Type II antibodies and immune complexes in diseases such as osteoarthritis and traumatised joints among horses.
Research Method
- The researchers focused on different horse joint diseases such as naturally occurring osteoarthritis (OA), osteochondritis dissecans (OCD), bone fracture, traumatised joints, synovitis, and infected joints.
- Each disease condition was compared with non-diseased control joints.
- Research measurements involved evaluating Serum and synovial fluid (SF) immune complexes and anti-collagen Type II antibodies. This was done using ELISA, a type of test that detects and measures antibodies in the blood.
Findings
- Increased anti-collagen Type II antibodies were observed in the synovial fluids of horses with osteoarthritis and traumatised joints compared to the control.
- Elevated levels of C1q-binding immune complexes were found in the synovial fluids of horses with OA, OCD, fractured articular bone, infected and traumatised joints when compared to control horses.
- No raised anti-collagen Type II antibodies or C1q-binding activity were detected in sera (the clear part of the blood) from any clinical group in the study.
Correlations
- In terms of correlations, the researchers identified connections in the synovial fluids between anti-collagen Type II antibodies and C1q-binding activity for the osteoarthritis, synovitis, and infected joint groups.
- However, within individual horses, no correlations were found between the SF and sera measurements of either anti-collagen II or C1q-binding activity.
Conclusion
- According to the study, the cause and effect relationship of these immunological findings couldn’t be determined since they are common to many types of horse joint disorders. Thus, they are likely not the starting factor in pathology.
Cite This Article
APA
Osborne AC, Carter SD, May SA, Bennett D.
(1995).
Anti-collagen antibodies and immune complexes in equine joint diseases.
Vet Immunol Immunopathol, 45(1-2), 19-30.
https://doi.org/10.1016/0165-2427(94)05325-m Publication
Researcher Affiliations
- Department of Veterinary Clinical Science and Animal Husbandry, Faculty of Veterinary Science, University of Liverpool, UK.
MeSH Terms
- Animals
- Antigen-Antibody Complex / analysis
- Antigen-Antibody Complex / blood
- Autoantibodies / analysis
- Autoantibodies / blood
- Collagen / immunology
- Complement C1q / immunology
- Enzyme-Linked Immunosorbent Assay / veterinary
- Horse Diseases / immunology
- Horses
- Joint Diseases / immunology
- Joint Diseases / veterinary
- Rabbits
- Synovial Fluid / immunology
Citations
This article has been cited 1 times.- Villasante A, Araneda OF, Behn C, Galleguillos M, Adarmes H. Antioxidant capacity and oxidative damage determination in synovial fluid of chronically damaged equine metacarpophalangeal joint. Vet Res Commun 2010 Feb;34(2):133-41.
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