Anti-Müllerian hormone as a biomarker for acute testicular degeneration caused by toxic insults to stallion testes.
Abstract: Recently, anti-Müllerian hormone (AMH) was validated as a reliable marker of testicular damage caused by various chemotherapy drugs in humans and in mice. In horses, the reference values of AMH concentrations in normal stallions, during different seasons of a year, have been recently reported. However, this hormone was not evaluated in subfertile or infertile stallions with testicular damage. Therefore, the objective of this study was to investigate the effects of experimentally induced testicular degeneration on the concentration of AMH in stallions. Severe but transient testicular degeneration was induced in six Miniature horse stallions, in two, separate experiments (three stallions in each experiment), by the administration of a single dose of the contraceptive compound RTI-4587-073(l). Six different stallions served as controls (three stallions in each experiment). Treated and control stallions were switched between the experiments. Concentrations of AMH were determined in 78 samples of blood plasma collected during the first experiment and in 24 samples collected during the second experiment. Furthermore, the expression of AMH in 30 samples of testicular parenchyma, collected from these stallions during the second experiment, was also evaluated, using immunohistochemistry (IHC) and objectively analyzed using computerized methods. During the first experiment, the concentrations of AMH in blood increased significantly in treated stallions (P < 0.05), reaching a 62-151% change from the baseline by day 10 after treatment, before gradually decreasing to the pretreatment levels. There was no change in blood AMH concentration in control stallions. Only a trend to increase AMH concentration was observed in treated stallions during the second experiment (P = 0.055). The labeling for immunoreactive AMH in the Sertoli cells gradually increased after treatment, which was confirmed by the significantly increased IHC optic density score value (P < 0.05) and significantly decreased percentage contribution of negative pixels at fourth week after treatment (P < 0.05). We concluded that AMH is a promising candidate as a biomarker of testicular damage in stallions caused by toxic insults that lead to testicular degeneration.
Copyright © 2018 Elsevier Inc. All rights reserved.
Publication Date: 2018-05-17 PubMed ID: 29800806DOI: 10.1016/j.theriogenology.2018.05.009Google Scholar: Lookup
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- Journal Article
Summary
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This research looks into the use of anti-Müllerian hormone (AMH) as a possible tool in identifying testicular damage in stallions, particularly when this damage is due to harmful substances causing testicular deterioration.
Objective of the Research
The main aim of this research was to:
- Investigate how induced testicular degeneration affects the concentration of anti-Müllerian hormone (AMH) in stallions.
Research Methodology
The research carried out two separate experiments involving six Miniature horse stallions. The methodology included:
- Causing severe but temporary testicular degeneration in six Miniature horse stallions through the administration of a contraceptive compound called RTI-4587-073(l).
- Using six other stallions as control subjects.
- Switching the control and treated stallions between the two experiments.
- Testing AMH concentrations in the blood plasma and testicular parenchyma of all involved stallions.
Results of the Study
The results of this study documented:
- Significant increases in blood AMH concentrations in stallions that were treated, indicating that rising AMH levels could be associated with testicular damage.
- Increased AMH expression in the Sertoli cells of stallions subjected to testicular degeneration, further suggesting a correlation between AMH levels and testicle health.
Conclusions
Based on the results, the research concluded that:
- Anti-Müllerian hormone could potentially serve as a valuable biomarker for identifying testicular damage in stallions, specifically damage that leads to testicular deterioration.
Cite This Article
APA
Pozor M, Conley AJ, Roser JF, Nolin M, Zambrano GL, Runyon SP, Kelleman AA, Macpherson ML.
(2018).
Anti-Müllerian hormone as a biomarker for acute testicular degeneration caused by toxic insults to stallion testes.
Theriogenology, 116, 95-102.
https://doi.org/10.1016/j.theriogenology.2018.05.009 Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA. Electronic address: pozorm@ufl.edu.
- Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA, 95616, United States.
- Department of Animal Science, University of California, Davis, CA, 95616, USA.
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
- Research Triangle Institute, 3040 Cornwallis Road, Research Triangle Park, NC, 27709, USA.
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
MeSH Terms
- Animals
- Anti-Mullerian Hormone / blood
- Biomarkers / blood
- Horse Diseases / metabolism
- Horse Diseases / pathology
- Horses
- Immunochemistry
- Infertility, Male / metabolism
- Infertility, Male / pathology
- Infertility, Male / veterinary
- Male
- Testicular Diseases / metabolism
- Testicular Diseases / pathology
- Testicular Diseases / veterinary
- Testis / pathology
Citations
This article has been cited 4 times.- Hamed MA, Amin YA, Mohamed RH, El-Adl M, Bazeed SM, Elnegiry AA, Shawki HH, Al-Lethie AA. Evaluation of chemical castration using intra-testicular injection of zinc gluconate into the testis of the male donkey versus surgical castration: antimullerian hormone as an endpoint marker. BMC Vet Res 2023 Sep 2;19(1):140.
- Zhang J, Campion S, Catlin N, Reagan WJ, Palyada K, Ramaiah SK, Ramanathan R. Circulating microRNAs as promising testicular translatable safety biomarkers: current state and future perspectives. Arch Toxicol 2023 Apr;97(4):947-961.
- Posastiuc FP, Rizzoto G, Constantin NT, Nicolae G, Chiers K, Diaconescu AI, Șerban AI, Van Soom A, Codreanu MD. Anti-Müllerian hormone as a diagnostic marker for testicular degeneration in dogs: insights from cryptorchid models. Front Vet Sci 2024;11:1481248.
- Waqas MS, Arroyo E, Tibary A. Diagnostic Approach to Equine Testicular Disorders. Vet Sci 2024 May 29;11(6).
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