Anti-oxidative status and semen quality during cooled storage in stallions.
Abstract: Activity of the anti-oxidative enzymes glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT), content of thiobarbituric acid reactive substances (TBARS) and SH-groups were determined in native stallion semen (n = 8 stallions). Semen was then diluted in Kenney extender, EquiPro((R)) extender either with or without addition of N-acetyl cysteine or phosphate-buffered saline (PBS) and stored for 72 h at 5 degrees C. Correlations between initial activity of enzymes and development of semen motility and membrane integrity were calculated. Activities of GSH-Px, SOD and CAT immediately after semen collections were 10.0 +/- 0.6 picokatals, 0.40 +/- 0.03 SOD units and 0.70 +/- 0.05 nanokatals/10(6) spermatozoa respectively. TBARS content was 0.06 +/- 0.01 nmol and SH-group content 1.7 +/- 0.5 mmol/10(6) spermatozoa. The loss of motile spermatozoa during storage did not differ between extenders. N-acetyl cysteine had no effect on semen motility and membrane integrity. The loss in membrane-intact spermatozoa was highest (P < 0.05) in semen diluted in PBS. Motility and membrane integrity after addition of extender were positively correlated with GSH-Px and CAT, indicating that anti-oxidative mechanisms contribute to the initial high percentage of motile and membrane-intact spermatozoa. However, in these samples the decrease in semen quality was most pronounced. No correlations existed between initial activity of anti-oxidative enzymes, peroxidation products and semen quality during storage. This indicates that once extender has been added, peroxidative damage to sperm membranes is not the predominant cause of losses in semen quality.
Publication Date: 2006-10-24 PubMed ID: 17054487DOI: 10.1111/j.1439-0442.2006.00879.xGoogle Scholar: Lookup
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- Journal Article
Summary
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This research aims to examine the effect of antioxidant enzymes on the quality and longevity of stored stallion semen. The study did not observe any significant correlation between the levels of antioxidant activity and the deterioration in semen quality during storage.
Research Methodology
- The researchers studied the activity of antioxidant enzymes such as glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) in the fresh semen from eight stallions.
- The semen samples were diluted using different extenders like Kenney extender and EquiPro((R)) and stored for 72 hours at 5 degrees Celsius.
- Some of the dilution extenders contained added N-acetyl cysteine or phosphate-buffered saline (PBS).
- The researchers monitored the decline in semen motility and membrane integrity over the storage period, which are important factors in determining the quality of the semen.
Results
- According to the results, the antioxidant enzymes GSH-Px, SOD, and CAT were active immediately after sperm collection. However, the study observed no correlation between the activity of these enzymes and semen quality during storage.
- The study also found that adding N-acetyl cysteine to the extender did not improve semen motility or membrane integrity.
- Semen diluted with PBS had the highest loss of membrane-intact spermatozoa, showing the deleterious effect of the addition of PBS.
- The researchers also found a positive correlation between GSH-Px and CAT activity, and the initial sperm motility and integrity, suggesting that antioxidant mechanisms might be beneficial in maintaining early sperm quality.
Conclusions
- In contrast to the benefits seen on initial sperm quality, the research found that those samples with high early antioxidant enzyme activity experienced the greatest deterioration in semen quality.
- The activity of antioxidant enzymes and the presence of peroxidation products did not have any evident influence on the quality of semen during storage.
- They concluded that peroxidative damage to sperm membranes is not the predominant cause of losses in semen quality during storage, once extender has been added.
Cite This Article
APA
Pagl R, Aurich C, Kankofer M.
(2006).
Anti-oxidative status and semen quality during cooled storage in stallions.
J Vet Med A Physiol Pathol Clin Med, 53(9), 486-489.
https://doi.org/10.1111/j.1439-0442.2006.00879.x Publication
Researcher Affiliations
- Department of Animal Breeding and Reproduction, Clinic for Obstetrics, Gynaecology and Andrology, University of Veterinary Science, Vienna, Austria.
MeSH Terms
- Animals
- Antioxidants / metabolism
- Cold Temperature
- Glutathione Peroxidase / metabolism
- Horses / physiology
- Lipid Peroxidation
- Male
- Oxidative Stress
- Semen / enzymology
- Semen / physiology
- Semen Preservation / methods
- Semen Preservation / veterinary
- Superoxide Dismutase / metabolism
- Thiobarbituric Acid Reactive Substances / metabolism
Citations
This article has been cited 1 times.- Zhu Z, Zeng Y, Zeng W. Cysteine improves boar sperm quality via glutathione biosynthesis during the liquid storage. Anim Biosci 2022 Feb;35(2):166-176.
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