Anti-pseudomonas activity of anti-lipopolysaccharide hyperimmune equine plasma.
Abstract: Passive immunotherapy with anti-lipopolysaccharide hyperimmune equine plasma (Anti-LPS) is effective in treating experimental Gram-negative bacterial infections. The bactericidal activity of anti-LPS towards five different Pseudomonas species, including two multiresistant Pseudomonas aeruginosa isolates was tested here, as well as the ability of anti-LPS to inhibit the quantitative chromogenic limulus amoebocyte lysate (LAL) assay. Anti-LPS caused a mean reduction of 84.4 +/- 3.2% (P less than 0.001) in the number of colony forming units (cfu) of all isolates, whereas saline and complement inactivated anti-LPS induced no reduction. Nonimmune control plasma caused a small reduction in % cfu but much less than anti-LPS hyperimmune plasma (13.5% vs 84.4%, P less than 0.001). In order to cause 99% inhibition of the LAL test of 5 ng/ml Pseudomonas aeruginosa LPS, IgG antibodies were required in 10(5)-fold excess. These results suggest that anti-LPS has potential in the therapy and prophylaxis of Gram-negative bacterial infections, especially where LPS is involved in the disease process.
Publication Date: 1987-04-01 PubMed ID: 3115643PubMed Central: PMC1542696
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study investigated the effectiveness of using anti-lipopolysaccharide hyperimmune equine plasma (Anti-LPS) as a passive immunotherapy method against multiple resistant Pseudomonas bacterial infections, demonstrating that it significantly reduces the number of colony-forming bacteria and inhibits their activity.
Study Method and Findings
- The researchers tested the bactericidal activity of anti-LPS on five different Pseudomonas species, specifically focusing on two resistant strains of Pseudomonas aeruginosa. They used a quantitative chromogenic limulus amoebocyte lysate (LAL) assay to measure the effectiveness of this intended therapy.
- The results indicated that anti-LPS caused an average reduction of about 84.4% in the colony-forming units (CFUs) of all tested bacterial isolates. In contrast, saline and complement-inactivated anti-LPS had no effect on the number of CFUs.
- The study further compared the effectiveness of anti-LPS with nonimmune control plasma. The control plasma only managed a minor reduction of colony-forming units (about 13.5%) which was significantly lower than the amount reduced by the anti-LPS hyperimmune plasma.
- To achieve a 99% inhibition rate on the LAL test of Pseudomonas aeruginosa LPS, the researchers concluded that IgG antibodies had to be present in a 10(5)-fold excess.
Implications of the Study
- This study suggests that anti-LPS could be a potential therapy and preventative measure against Gram-negative bacterial infections, especially in cases where lipopolysaccharide (LPS) is involved in the disease process.
- The effectiveness of anti-LPS against even resistant strains of Pseudomonas aeruginosa indicates its promise in combating rising antibiotic resistance in different bacterial species.
- However, the requirement for IgG antibodies to be in 10(5)-fold excess for significant bacterial inhibition underscores the need for optimal conditions and concentrated use of anti-LPS for it to be truly effective.
Cite This Article
APA
Wells M, Gaffin SL.
(1987).
Anti-pseudomonas activity of anti-lipopolysaccharide hyperimmune equine plasma.
Clin Exp Immunol, 68(1), 86-92.
Publication
Researcher Affiliations
- Department of Physiology, University of Natal Medical School, Congella, South Africa.
MeSH Terms
- Animals
- Blood Bactericidal Activity
- Horses
- Immunization, Passive
- Immunoglobulin G / immunology
- Limulus Test
- Lipopolysaccharides / immunology
- Pseudomonas / immunology
- Pseudomonas aeruginosa / immunology
References
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