Antibacterial Activities of Bacillus amyloliquefaciens DQB-1 Isolated From the Cecum of Dezhou Donkeys.

The research looks at Bacillus amyloliquefaciens DQB-1, a bacteria isolated from a donkey’s cecum, and its antibacterial activities that could potentially aid in preventing infectious diseases in donkey farms.

Isolation and Identification of Bacteria

• The researchers studied the microorganisms in the cecum of Dezhou donkeys as these play essential roles in their health and growth, similarly to microorganisms found in the cow’s rumen.
• They isolated a strain of Bacillus amyloliquefaciens (DQB-1) which exhibited antibacterial properties.

Assessment of Antibacterial Activity

• The properties of the isolated strain were studied with special attention to its antibacterial activity, the substances it produces that exhibit this activity, and its stability.
• Tests showed that Bacillus amyloliquefaciens DQB-1 displayed protease production activity. Protease is a type of enzyme that degrades proteins, including those that could be harmful to the host organism, thereby contributing to its antibacterial properties.
• It was found that this strain could significantly inhibit the growth of other bacterial and fungal pathogens, acting as a potential deterrent against infectious diseases.

Extraction and Storage of Antibacterial Substances

• The study noted that the strongest antibacterial substances are obtained after 24 hours of growth.
• They also studied the optimal storage conditions for these extracts and found that a temperature of -20 degrees Celsius was most suitable.

Potential Applications

• Due to Bacillus amyloliquefaciens DQB-1’s strong antibacterial activity, its ability to produce protease, and its overall stability, it is seen as a viable candidate for developing into a probiotic preparation.
• This probiotic preparation could potentially be used to prevent infectious diseases in donkey farms, offering a means to increase animal health and productivity, and reducing the need for antibiotic use.