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Home / Equine Research Bank / Materials (Basel) / Antibacterial Properties of Biodegradable Silver Nanoparticl...
Materials (Basel, Switzerland)2022; 15(3); doi: 10.3390/ma15031269

Antibacterial Properties of Biodegradable Silver Nanoparticle Foils Based on Various Strains of Pathogenic Bacteria Isolated from the Oral Cavity of Cats, Dogs and Horses.

Abstract: Frequent occurrence of microbial resistance to biocides makes it necessary to find alternative antimicrobial substances for modern veterinary medicine. The aim of this study was to obtain biodegradable silver nanoparticle-containing (AgNPs) foils synthesized using non-toxic chemicals and evaluation of their activity against bacterial pathogens isolated from oral cavities of cats, dogs and horses. Silver nanoparticle foils were synthesized using sodium alginate, and glucose, maltose and xylose were used as reducing agents. The sizes of AgNPs differed depending on the reducing agent used (xylose < maltose < glucose). Foil without silver nanoparticles was used as control. Bacterial strains were isolated from cats, dogs and horses by swabbing their oral cavities. Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and extended-spectrum beta-lactamase (ESBL) producing E. coli were isolated on selective chromogenic microbiological media. The bactericidal effect of AgNPs foils obtained using non-toxic chemical compounds against E. coli, ESBL, S. aureus and MRSA isolated from oral cavities of selected animals was confirmed in this study. No statistically significant differences were observed between the foils obtained with different reducing agents. Therefore, all types of examined foils proved to be effective against the isolated bacteria.
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Publication Date: 2022-02-08 PubMed ID: 35161213PubMed Central: PMC8840282DOI: 10.3390/ma15031269Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper is about the creation of biodegradable silver nanoparticle (AgNPs) foils and their effectiveness in killing bacteria isolated from the mouths of cats, dogs, and horses. Different non-toxic reducing agents were used in the foils’ synthesis and the results indicated that all foils were successful in eradicating the bacteria.

About the Study

  • The study has been motivated by the increasing realization of bacterial resistance to typical antimicrobial substances. In response, the researchers aimed to develop a new material – biodegradable silver nanoparticle (AgNPs) foils – using non-toxic chemicals. The foils were then tested against bacteria from the mouths of cats, dogs, and horses.
  • Silver nanoparticle foils were created using sodium alginate. Then, different non-toxic reducing agents (glucose, maltose, and xylose) were employed in the process. The sizes of the nanoparticles varied according to the reducing agent used. To compare the effects, a control was established by the scientists using a foil without silver nanoparticles.

Collection and Identification of Bacterial Strains

  • To collect suitable bacteria for the study, the researchers took samples from the oral cavities of cats, dogs, and horses.
  • The bacteria then isolated and identified include Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and strains producing extended-spectrum beta-lactamase (ESBL). They were cultivated on selective chromogenic microbiological media.

Effectiveness of AgNPs Foils

  • In the key component of the current study, the synthesized foils were applied to the bacteria to determine its bactericidal properties, that is, the ability to kill bacteria.
  • The findings of the research provided confirmation that the AgNPs foils synthesized with non-toxic chemical compounds were successful in eradicating the selected bacterial strains.
  • Additionally, the research reported that no statistically significant differences were observed among the foils produced with different non-toxic reducing agents, indicating that all examined types were effective against the bacteria.

Cite This Article

APA
Rutkowski M, Krzemińska-Fiedorowicz L, Khachatryan G, Kabacińska J, Tischner M, Suder A, Kulik K, Lenart-Boroń A. (2022). Antibacterial Properties of Biodegradable Silver Nanoparticle Foils Based on Various Strains of Pathogenic Bacteria Isolated from the Oral Cavity of Cats, Dogs and Horses. Materials (Basel), 15(3). https://doi.org/10.3390/ma15031269

Publication

Materials (Basel, Switzerland)
ISSN: 1996-1944
NlmUniqueID: 101555929
Country: Switzerland
Language: English
Volume: 15
Issue: 3

Researcher Affiliations

Rutkowski, Miłosz
  • Scientific Circle of Biotechnologists "Helisa", Microbiology Section, Department of Microbiology and Biomonitoring, Faculty of Agriculture and Economics, University of Agriculture in Krakow, 30-059 Krakow, Poland.
Krzemińska-Fiedorowicz, Lidia
  • Faculty of Food Technology, University of Agriculture in Krakow, 30-149 Krakow, Poland.
Khachatryan, Gohar
  • Faculty of Food Technology, University of Agriculture in Krakow, 30-149 Krakow, Poland.
Kabacińska, Julia
  • "Przychodnia Weterynaryjna Uniwersytecka" Veterinary Clinic, University Center of Veterinary Medicine, University of Agriculture in Krakow, 30-251 Krakow, Poland.
Tischner, Marek
  • Department of Animal Reproduction, Anatomy and Genomics, Faculty of Animal Science, University of Agriculture in Krakow, 30-059 Krakow, Poland.
Suder, Aleksandra
  • Department of Microbiology and Biomonitoring, Faculty of Agriculture and Economics, University of Agriculture in Krakow, 30-059 Krakow, Poland.
Kulik, Klaudia
  • Department of Microbiology and Biomonitoring, Faculty of Agriculture and Economics, University of Agriculture in Krakow, 30-059 Krakow, Poland.
Lenart-Boroń, Anna
  • Department of Microbiology and Biomonitoring, Faculty of Agriculture and Economics, University of Agriculture in Krakow, 30-059 Krakow, Poland.

Grant Funding

  • Statutory measures of the University of Agriculture in Kraków / University of Agriculture in Krakow

Conflict of Interest Statement

The authors declare no conflict of interest.

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