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Home / Equine Research Bank / Clin Vaccine Immunol / Antibodies to a novel leptospiral protein, LruC, in the eye ...
Clinical and vaccine immunology : CVI2012; 19(3); 452-456; doi: 10.1128/CVI.05524-11

Antibodies to a novel leptospiral protein, LruC, in the eye fluids and sera of horses with Leptospira-associated uveitis.

Abstract: Screening of an expression library of Leptospira interrogans with eye fluids from uveitic horses resulted in identification of a novel protein, LruC. LruC is located in the inner leaflet of the leptospiral outer membrane, and an lruC gene was detected in all tested pathogenic L. interrogans strains. LruC-specific antibody levels were significantly higher in eye fluids and sera of uveitic horses than healthy horses. These findings suggest that LruC may play a role in equine leptospiral uveitis.
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Publication Date: 2012-01-11 PubMed ID: 22237897PubMed Central: PMC3294619DOI: 10.1128/CVI.05524-11Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the discovery of a novel protein, LruC, associated with Leptospira bacteria, and its possible role in equine leptospiral uveitis, an eye infection in horses.

Identification of a New Protein

  • The researchers performed screening on an “expression library” of Leptospira interrogans, a type of bacteria. This process resulted in finding a previously unknown protein, named LruC.

LruC’s Location and Presence across Strains

  • LruC was found to be located in the inner leaflet of the leptospiral outer membrane, which is essentially the outermost layer of the bacteria.
  • The gene for LruC was detected in all the tested pathogenic Leptospira interrogans strains implying that it is a common feature among this particular species.

Antibodies Level in Uveitic Horses

  • The researchers analyzed eye fluids and sera (the clear liquid that can be separated from clotted blood) from horses suffering from uveitis, which is an inflammation of the middle layer of the eye.
  • Levels of LruC-specific antibodies (proteins produced by the immune system to neutralize harmful substances) were significantly higher in these uveitic horses as compared to healthy horses. This indicates that an immune response had been triggered in the horses against the LruC protein.

Implications of the Findings

  • The high antibody levels in uveitic horses point towards a potential role of the LruC protein in equine leptospiral uveitis. However, what that role might be is not clarified in the abstract and would likely be addressed in the full article.
  • This discovery opens up potential new avenues for diagnosing and treating leptospiral infections in horses, as the LruC protein could form the basis of new diagnostic tests or targeted treatments.

Cite This Article

APA
Verma A, Matsunaga J, Artiushin S, Pinne M, Houwers DJ, Haake DA, Stevenson B, Timoney JF. (2012). Antibodies to a novel leptospiral protein, LruC, in the eye fluids and sera of horses with Leptospira-associated uveitis. Clin Vaccine Immunol, 19(3), 452-456. https://doi.org/10.1128/CVI.05524-11

Publication

Clinical and vaccine immunology : CVI
ISSN: 1556-679X
NlmUniqueID: 101252125
Country: United States
Language: English
Volume: 19
Issue: 3
Pages: 452-456

Researcher Affiliations

Verma, Ashutosh
  • M. H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA. ashutosh.verma@uky.edu
Matsunaga, James
    Artiushin, Sergey
      Pinne, Marija
        Houwers, Dirk J
          Haake, David A
            Stevenson, Brian
              Timoney, John F

                MeSH Terms

                • Animals
                • Antibodies, Bacterial / analysis
                • Antibodies, Bacterial / blood
                • Antigens, Bacterial / genetics
                • Antigens, Bacterial / immunology
                • Bacterial Outer Membrane Proteins / genetics
                • Bacterial Outer Membrane Proteins / immunology
                • Body Fluids / immunology
                • DNA, Bacterial / chemistry
                • DNA, Bacterial / genetics
                • Eye / immunology
                • Horse Diseases / immunology
                • Horse Diseases / pathology
                • Horses
                • Leptospira / immunology
                • Leptospira / pathogenicity
                • Leptospirosis / immunology
                • Leptospirosis / pathology
                • Leptospirosis / veterinary
                • Molecular Sequence Data
                • Sequence Analysis, DNA
                • Uveitis / immunology
                • Uveitis / pathology
                • Uveitis / veterinary

                Grant Funding

                • R01 AI034431 / NIAID NIH HHS
                • R21 AI034431 / NIAID NIH HHS
                • R29 AI034431 / NIAID NIH HHS
                • AI-34431 / NIAID NIH HHS

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                Citations

                This article has been cited 8 times.
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