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Home / Equine Research Bank / Vet Parasitol / Antibody levels by indirect ELISA test in Trypanosoma evansi...
Veterinary parasitology2003; 111(1); 59-63; doi: 10.1016/s0304-4017(02)00331-x

Antibody levels by indirect ELISA test in Trypanosoma evansi infected horses following treatment with quinapyramine sulphate.

Abstract: An ELISA test was used to determine the persistence of antibody levels in horses following treatment for Trypanosoma evansi. In 17 horses with T. evansi from two farms treated and cured with quinapyramine sulphate, ELISA antibody levels fell progressively post-treatment, but remained with positive results for 22.6 months in one horse, 12.8 months in a second, 4.1 months in another four and 2.3 months in three, whilst the rest became negative at 2.3 months. In two horses that suffered a post-treatment infection relapse the decrease in ELISA levels was only temporary, and a new increase in antibody levels was proven. The follow-up of these antibody levels could prove useful in clinical cases and in epidemiological studies, as well as for assessing the efficacy of drug treatment.
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Publication Date: 2003-01-14 PubMed ID: 12523979DOI: 10.1016/s0304-4017(02)00331-xGoogle Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates how long antibodies for Trypanosoma evansi stay in horses after being treated and cured with quinapyramine sulphate, using the ELISA test as a measurement tool. The study found that the antibodies remain detectable for variable periods of time, with some remaining for nearly 2 years.

Overview of Findings

  • The study involved 17 horses from two different farms which had been infected with Trypanosoma evansi and treated with quinapyramine sulphate.
  • Using the ELISA test, the researchers measured the level of antibodies in these horses after the treatment.
  • They found that antibody levels decreased progressively after treatment, but these antibodies could still be detected for varying lengths of time in different horses.
  • The longest detection time was 22.6 months in one horse, while the shortest was 2.3 months in several others.

Case of Treatment Relapse

  • The research also found two incidences of treatment relapse, where the horses initially showed a decrease in antibody levels, but it was followed by a subsequent increase.
  • This indicates that the infection had returned in these horses.

Implications of Findings

  • The findings have potential clinical implications. The persistence of antibodies after treatment indicates that these horses may still be carriers of the disease after apparent cure.
  • Further, the results could influence epidemiological studies as the levels of these antibodies in different populations over time can help understand the spread and prevalence of the disease.
  • The results also have potential implications for assessing drug efficacy. A faster decrease in antibody level could be an indication that the treatment is more effective.

Conclusion

  • This study provides valuable insights into the behaviour of antibodies in horses following treatment for Trypanosoma evansi with quinapyramine sulphate. These findings may play a crucial role in developing better treatments, designing more accurate epidemiological studies, and improving overall understanding of the disease.

Cite This Article

APA
Monzon CM, Mancebo OA, Russo AM. (2003). Antibody levels by indirect ELISA test in Trypanosoma evansi infected horses following treatment with quinapyramine sulphate. Vet Parasitol, 111(1), 59-63. https://doi.org/10.1016/s0304-4017(02)00331-x

Publication

Veterinary parasitology
ISSN: 0304-4017
NlmUniqueID: 7602745
Country: Netherlands
Language: English
Volume: 111
Issue: 1
Pages: 59-63

Researcher Affiliations

Monzon, C M
  • Centro de Diagnóstico e Investigaciones Veterinarias Formosa, Cátedra de Parasitología-Facultad de Ciencias de la Salud-Universidad Nacional de Formosa, Ruta Nac. No. 11, km 1164, C.C. 73 (P3600BCW), Formosa, Argentina.
Mancebo, O A
    Russo, A M

      MeSH Terms

      • Animals
      • Antibodies, Protozoan / blood
      • Enzyme-Linked Immunosorbent Assay
      • Horse Diseases / drug therapy
      • Horse Diseases / immunology
      • Horse Diseases / parasitology
      • Horses
      • Quinolinium Compounds / therapeutic use
      • Time Factors
      • Trypanocidal Agents / therapeutic use
      • Trypanosoma / immunology
      • Trypanosomiasis / drug therapy
      • Trypanosomiasis / immunology
      • Trypanosomiasis / veterinary

      Citations

      This article has been cited 8 times.
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        doi: 10.1155/2019/2964639pubmed: 31886196google scholar: lookup
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        pubmed: 26623297
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        doi: 10.1155/2015/361037pubmed: 26101772google scholar: lookup
      6. Birhanu H, Fikru R, Said M, Kidane W, Gebrehiwot T, Hagos A, Alemu T, Dawit T, Berkvens D, Goddeeris BM, Büscher P. Epidemiology of Trypanosoma evansi and Trypanosoma vivax in domestic animals from selected districts of Tigray and Afar regions, Northern Ethiopia.. Parasit Vectors 2015 Apr 9;8:212.
        doi: 10.1186/s13071-015-0818-1pubmed: 25889702google scholar: lookup
      7. Yadav SC, Kumar R, Manuja A, Goyal L, Gupta AK. Early detection of Trypanosoma evansi infection and monitoring of antibody levels by ELISA following treatment.. J Parasit Dis 2014 Mar;38(1):124-7.
        doi: 10.1007/s12639-012-0204-2pubmed: 24505190google scholar: lookup
      8. Ranjithkumar M, Saravanan BC, Yadav SC, Kumar R, Singh R, Dey S. Neurological trypanosomiasis in quinapyramine sulfate-treated horses--a breach of the blood-brain barrier?. Trop Anim Health Prod 2014 Feb;46(2):371-7.
        doi: 10.1007/s11250-013-0498-9pubmed: 24197687google scholar: lookup
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