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Home / Equine Research Bank / Can J Vet Res / Antibody response of horses to Rhodococcus equi antigens.
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire1987; 51(3); 301-305;

Antibody response of horses to Rhodococcus equi antigens.

Abstract: The antigens extracted from strains belonging to seven capsular serotypes of Rhodococcus equi, as well as from two wild strains isolated from pneumonic foals, were examined. Whole-cell antigens and soluble products present in broth culture supernatants were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, electroblotted onto nitrocellulose, and stained with serum from hyperimmunized rabbits or foals. Foal sera used included sera from pneumonic animals with known titer to equi factors; from animals bled monthly on a farm with enzootic pneumonia, and from animals bled monthly on a farm with no history of R. equi pneumonia. The humoral response of foals to somatic antigen preparations was negligible, with few differences noted between sera from healthy, subclinically affected, and sick foals. The humoral response to R. equi broth culture supernatant products appeared more marked and was related to equi factor antibody titer. These findings suggest that the humoral response to R. equi whole-cell antigens is unimportant in protection against disease, which is consistent with the behavior of the organism as a facultative intracellular pathogen.
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Publication Date: 1987-07-01 PubMed ID: 3651884PubMed Central: PMC1255327
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study explores and compares the antibody response in horses to different strains and products of the bacterium Rhodococcus equi, arguing that the humoral immune response to whole-cell antigens from this bacterium is not crucial for disease protection.

Overview of the Research Study

  • The research involved evaluating the antigens derived from seven different serotypes of Rhodococcus equi and two wild strains isolated from foals with pneumonia. Antigens are substances that, when introduced into the body, cause an immune response, typically the production of antibodies.
  • Two different types of antigens were looked at: whole-cell antigens from the bacteria, and soluble products found in the growth medium of the bacterial culture.
  • The procedure used to separate the antigens was sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), a common laboratory method for separating proteins by their molecular weight. After separation using this method, the antigens were transferred onto a membrane and then stained with serum from hyperimmunized rabbits or foals.

Results and implications

  • Results showed that there was a negligible humoral response (part of the immune system that involves the production of antibodies) in foals to the somatic antigen preparations. No significant differences were observed between healthy, mildly affected, or sick foals in this respect.
  • The humoral response to products from the growth medium of R. equi was more pronounced and showed a connection with the titer of equi factor antibodies, which are specific antibodies to R. equi.
  • These findings lead the researchers to suggest that the humoral response to the whole-cell R. equi antigens plays a minor role in protection against the disease. This conclusion is consistent with the known behavior of R. equi as a facultative intracellular pathogen, meaning that it can survive both inside and outside cells.

Cite This Article

APA
Chirino-Trejo JM, Prescott JF. (1987). Antibody response of horses to Rhodococcus equi antigens. Can J Vet Res, 51(3), 301-305.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 0830-9000
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 51
Issue: 3
Pages: 301-305

Researcher Affiliations

Chirino-Trejo, J M
  • Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph.
Prescott, J F

    MeSH Terms

    • Animals
    • Antibodies, Bacterial / biosynthesis
    • Antibody Formation
    • Antigens, Bacterial / immunology
    • Horses / immunology
    • Rhodococcus / immunology

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    Citations

    This article has been cited 5 times.
    1. van der Geize R, Grommen AW, Hessels GI, Jacobs AA, Dijkhuizen L. The steroid catabolic pathway of the intracellular pathogen Rhodococcus equi is important for pathogenesis and a target for vaccine development.. PLoS Pathog 2011 Aug;7(8):e1002181.
      doi: 10.1371/journal.ppat.1002181pubmed: 21901092google scholar: lookup
    2. Chirino-Trejo JM, Prescott JF. Polyacrylamide gel electrophoresis of whole-cell preparations of Rhodococcus equi.. Can J Vet Res 1987 Jul;51(3):297-300.
      pubmed: 3651883
    3. Takai S, Koike K, Ohbushi S, Izumi C, Tsubaki S. Identification of 15- to 17-kilodalton antigens associated with virulent Rhodococcus equi.. J Clin Microbiol 1991 Mar;29(3):439-43.
      doi: 10.1128/jcm.29.3.439-443.1991pubmed: 2037660google scholar: lookup
    4. Prescott JF. Rhodococcus equi: an animal and human pathogen.. Clin Microbiol Rev 1991 Jan;4(1):20-34.
      doi: 10.1128/CMR.4.1.20pubmed: 2004346google scholar: lookup
    5. Tkachuk-Saad O, Prescott J. Rhodococcus equi plasmids: isolation and partial characterization.. J Clin Microbiol 1991 Dec;29(12):2696-700.
      doi: 10.1128/jcm.29.12.2696-2700.1991pubmed: 1757535google scholar: lookup
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