Antigen-antibody crossed electrophoretic studies and quantitative comparisons of serum transferrin types in horses.
Abstract: Selected transferrin phenotypes from 14 horses were investigated by antigen-antibody crossed electrophoresis. Horse sera were subjected to starch gel electrophoresis followed by right angle electrophoresis in agarose gels containing rabbit produced anti-horse transferrin. This technique gave an additional zone in the front as compared with 2 transferrin zones seen after ordinary starch gel electrophoresis. Comparisons of transferrin concentrations in horse sera were performed by an immunodiffusion technique. Values were related to a chosen reference serum. A total of 372 horses (210 Norwegian Trotters and 162 Warmblood Trotters) were investigated. There were considerable variations among horses, with the highest transferrin value 196% and the lowest 55% of the standard. There were highly significant differences between average values of certain transferrin phenotypes, the highest being TfFF and the lowest TfDO, TfFO and TfRR. In animals with the same transferrin phenotype, the average transferrin values were significantly higher in foals than in mares. No difference in transferrin values were found between sexes. Utvalgte transferrin-typer fra 14 hester ble undersøkt ved antigen-antistoff-elektroforese. Hesteserum ble undersøkt ved hjelp av stivelsesgel-elektroforese etterfulgt av elektroforese på agarose gel som inneholdt antihest-transferrin fremstillet på kanin. Denne teknikk ga en ekstra sone i fronten sammenlignet med to transferrinsoner som sees etter vanlig stivelsesgel-elektroforese. Sammenligninger av transferrin-konsentrasjoner i hestesera ble utført ved anvendelse av Mancini et al.’s immundiffusjonsteknikk. Relative verdier i forhold til et valgt referanseserum ble bestemt for i alt 372 hester (210 norske travere og 162 varmblods travere). Det var betydelig variasjon mellom hestene, den høyeste transferrinverdi var 196% og den laveste 55%. Mellom visse transferrin-fenotyper var det høysignifikante forskjeller mellom middelverdier, TfFF var den høyeste og TfDO, TfFO og TfRR de laveste. Hos dyr med samme transferrin-fenotype var middelverdiene signifikant høyere hos føll enn hos hopper. Det ble ikke funnet noen forskjell på transferrin-verdier mellom kjønnene.
Publication Date: 1981-01-01 PubMed ID: 7304365PubMed Central: PMC8300493DOI: 10.1186/BF03547514Google Scholar: Lookup
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- Journal Article
Summary
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The study focuses on investigating different types of transferrin, a protein that transports iron in the body, in horses using a specialized technique known as antigen-antibody crossed electrophoresis. The transferrin concentrations were compared using an immunodiffusion technique in a sample of 372 horses, and variations among the horses were observed.
Methodology of Study
- The research started by selecting transferrin phenotypes from a total of 14 horses. The selected phenotypes were then subjected to a specialized technique known as antigen-antibody crossed electrophoresis.
- This technique involved treating horse serum with starch gel electrophoresis. They then subjected it to right angle electrophoresis in agarose gel, infused with rabbit produced anti-horse transferrin.
- This process led to an additional zone in the front, compared to the two transferrin zones that one can normally notice after standard starch gel electrophoresis.
Comparative Process and Results
- The comparison of transferrin concentrations in horse serum was done using an immunodiffusion technique, and the values were set against a selected reference serum.
- The total sample size included 372 horses, amongst which 210 were Norwegian Trotters and 162 were Warmblood Trotters.
- The study showed a substantial variation among horses, with the highest transferrin value at 196% and the lowest at 55% of the standard value.
- Significant differences were noted in the average values of certain transferrin phenotypes, with the highest average noticed in TfFF and the lowest in TfDO, TfFO, and TfRR phenotypes.
- When comparing animals with the same transferrin phenotype, the average transferrin values were significantly higher in foals (young horses) than in mares (adult female horses).
- However, no significant difference was discerned in transferrin values between different sexes.
In a nutshell, the study observes considerable variations in transferrin concentration levels among horses, highlighting the importance of sex and developmental stages (foal or mare) in determining these levels. The iron transport protein’s levels appear to be naturally higher in young horses, irrespective of their gender.
Cite This Article
APA
Ek N.
(1981).
Antigen-antibody crossed electrophoretic studies and quantitative comparisons of serum transferrin types in horses.
Acta Vet Scand, 22(2), 246-259.
https://doi.org/10.1186/BF03547514 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Female
- Horses / blood
- Horses / genetics
- Immunoelectrophoresis
- Male
- Transferrin / blood
- Transferrin / genetics
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Citations
This article has been cited 1 times.- Ek N. Concentration of serum transferrin in sick horses and its relationship to serum albumin content. Acta Vet Scand 1981;22(2):260-71.
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