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Acta veterinaria Scandinavica1981; 22(2); 246-259; doi: 10.1186/BF03547514

Antigen-antibody crossed electrophoretic studies and quantitative comparisons of serum transferrin types in horses.

Abstract: Selected transferrin phenotypes from 14 horses were investigated by antigen-antibody crossed electrophoresis. Horse sera were subjected to starch gel electrophoresis followed by right angle electrophoresis in agarose gels containing rabbit produced anti-horse transferrin. This technique gave an additional zone in the front as compared with 2 transferrin zones seen after ordinary starch gel electrophoresis. Comparisons of transferrin concentrations in horse sera were performed by an immunodiffusion technique. Values were related to a chosen reference serum. A total of 372 horses (210 Norwegian Trotters and 162 Warmblood Trotters) were investigated. There were considerable variations among horses, with the highest transferrin value 196% and the lowest 55% of the standard. There were highly significant differences between average values of certain transferrin phenotypes, the highest being TfFF and the lowest TfDO, TfFO and TfRR. In animals with the same transferrin phenotype, the average transferrin values were significantly higher in foals than in mares. No difference in transferrin values were found between sexes. Utvalgte transferrin-typer fra 14 hester ble undersøkt ved antigen-antistoff-elektroforese. Hesteserum ble undersøkt ved hjelp av stivelsesgel-elektroforese etterfulgt av elektroforese på agarose gel som inneholdt antihest-transferrin fremstillet på kanin. Denne teknikk ga en ekstra sone i fronten sammenlignet med to transferrinsoner som sees etter vanlig stivelsesgel-elektroforese. Sammenligninger av transferrin-konsentrasjoner i hestesera ble utført ved anvendelse av Mancini et al.’s immundiffusjonsteknikk. Relative verdier i forhold til et valgt referanseserum ble bestemt for i alt 372 hester (210 norske travere og 162 varmblods travere). Det var betydelig variasjon mellom hestene, den høyeste transferrinverdi var 196% og den laveste 55%. Mellom visse transferrin-fenotyper var det høysignifikante forskjeller mellom middelverdier, TfFF var den høyeste og TfDO, TfFO og TfRR de laveste. Hos dyr med samme transferrin-fenotype var middelverdiene signifikant høyere hos føll enn hos hopper. Det ble ikke funnet noen forskjell på transferrin-verdier mellom kjønnene.
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Publication Date: 1981-01-01 PubMed ID: 7304365PubMed Central: PMC8300493DOI: 10.1186/BF03547514Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study focuses on investigating different types of transferrin, a protein that transports iron in the body, in horses using a specialized technique known as antigen-antibody crossed electrophoresis. The transferrin concentrations were compared using an immunodiffusion technique in a sample of 372 horses, and variations among the horses were observed.

Methodology of Study

  • The research started by selecting transferrin phenotypes from a total of 14 horses. The selected phenotypes were then subjected to a specialized technique known as antigen-antibody crossed electrophoresis.
  • This technique involved treating horse serum with starch gel electrophoresis. They then subjected it to right angle electrophoresis in agarose gel, infused with rabbit produced anti-horse transferrin.
  • This process led to an additional zone in the front, compared to the two transferrin zones that one can normally notice after standard starch gel electrophoresis.

Comparative Process and Results

  • The comparison of transferrin concentrations in horse serum was done using an immunodiffusion technique, and the values were set against a selected reference serum.
  • The total sample size included 372 horses, amongst which 210 were Norwegian Trotters and 162 were Warmblood Trotters.
  • The study showed a substantial variation among horses, with the highest transferrin value at 196% and the lowest at 55% of the standard value.
  • Significant differences were noted in the average values of certain transferrin phenotypes, with the highest average noticed in TfFF and the lowest in TfDO, TfFO, and TfRR phenotypes.
  • When comparing animals with the same transferrin phenotype, the average transferrin values were significantly higher in foals (young horses) than in mares (adult female horses).
  • However, no significant difference was discerned in transferrin values between different sexes.

In a nutshell, the study observes considerable variations in transferrin concentration levels among horses, highlighting the importance of sex and developmental stages (foal or mare) in determining these levels. The iron transport protein’s levels appear to be naturally higher in young horses, irrespective of their gender.

Cite This Article

APA
Ek N. (1981). Antigen-antibody crossed electrophoretic studies and quantitative comparisons of serum transferrin types in horses. Acta Vet Scand, 22(2), 246-259. https://doi.org/10.1186/BF03547514

Publication

Acta veterinaria Scandinavica
ISSN: 0044-605X
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 22
Issue: 2
Pages: 246-259

Researcher Affiliations

Ek, N

    MeSH Terms

    • Animals
    • Female
    • Horses / blood
    • Horses / genetics
    • Immunoelectrophoresis
    • Male
    • Transferrin / blood
    • Transferrin / genetics

    References

    This article includes 19 references
    1. ASHTON GC. Serum protein variations in horses.. Nature 1958 Oct 11;182(4641):1029-30.
      doi: 10.1038/1821029a0pubmed: 13590217google scholar: lookup
    2. Baer A. Le polymorphisme biochemique des transferrines chez le cheval. .
    3. Bell OF, Carrell RW. Basis of the defect in alpha-1-antitrypsin deficiency.. Nature 1973 Jun 15;243(5407):410-1.
      doi: 10.1038/243410a0pubmed: 4542721google scholar: lookup
    4. Braend M, Stormont C. Studies on hemoglobin and transferrin types of horses. Nord. Vet.-Med. 1964;16:31–37.
    5. Ek N. Serum levels of the immunoglobulins IgG and IgG(T) in horses.. Acta Vet Scand 1974;15(4):609-19.
      doi: 10.1186/BF03547230pmc: PMC8407179pubmed: 4218064google scholar: lookup
    6. Ek N. The characterization of equine prealbumin (Pr) proteins by antigen-antibody crossed electrophoresis.. Acta Vet Scand 1979;20(2):180-90.
      doi: 10.1186/BF03546610pmc: PMC8322906pubmed: 484403google scholar: lookup
    7. Ek N, Braend M. Quantitative comparisons of acidic prealbumin (PR) phenotypes in horses.. Acta Vet Scand 1980;21(3):380-8.
      doi: 10.1186/BF03546870pmc: PMC8317704pubmed: 7446321google scholar: lookup
    8. Fagerhol MK. Quantitative studies on the inherited variants of serum alpha-1-antitrypsin.. Scand J Clin Lab Invest 1969 Feb;23(1):97-103.
      doi: 10.3109/00365516909078092pubmed: 5363442google scholar: lookup
    9. Holmberg C G, Laurell C-B. Studies on the capacity of serum to bind iron. Acta physiol. scand. 1945;10:307–319.
      doi: 10.1111/j.1748-1716.1945.tb00314.xgoogle scholar: lookup
    10. Jeppsson JO, Laurell CB, Nosslin B, Cox DW. Catabolic rate of alpha1-antitrypsin of of Pi types S, and MMalton and of asialylated M-protein in man.. Clin Sci Mol Med 1978 Jul;55(1):103-7.
      pubmed: 307478doi: 10.1042/cs0550103google scholar: lookup
    11. KISTLER P, NITSCHMANN H, WYTTENBACH A, STUDER M, NIEDEROST C, MAUERHOFER M. [Human siderophilin: isolation from blood plasma and plasma fractions by rivanol, analytical determination and crystallization].. Vox Sang 1960 Sep;5:403-15.
      doi: 10.1159/000478585pubmed: 13756425google scholar: lookup
    12. Laurell CB, Nosslin B, Jeppsson JO. Catabolic rate of alpha1-antitrypsin of Pi type M and Z in man.. Clin Sci Mol Med 1977 May;52(5):457-61.
      pubmed: 301079doi: 10.1042/cs0520457google scholar: lookup
    13. Makimura S, Tomoda I, Usui K. Quantitative studies on immunoglobulins and transferrin in equine serum.. Nihon Juigaku Zasshi 1975 Apr;37(2):187-98.
      doi: 10.1292/jvms1939.37.187pubmed: 1237744google scholar: lookup
    14. Mancini G, Carbonara AO, Heremans JF. Immunochemical quantitation of antigens by single radial immunodiffusion.. Immunochemistry 1965 Sep;2(3):235-54.
      doi: 10.1016/0019-2791(65)90004-2pubmed: 4956917google scholar: lookup
    15. Ritchie RF, Smith R. Immunofixation. I. General principles and application to agarose gel electrophoresis.. Clin Chem 1976 Apr;22(4):497-9.
      doi: 10.1093/clinchem/22.4.497pubmed: 56244google scholar: lookup
    16. Thorén-Tolling K, Martinsson K. On the transferrin concentration in serum of sows and growing pigs and in sow colostrum.. Acta Vet Scand 1974;15(1):120-34.
      doi: 10.1186/BF03547499pmc: PMC8407248pubmed: 4208445google scholar: lookup
    17. Thoren-Tolling K. Serumprotein variationer vid patologiska tilstånd hos hest och hund. Svensk Vet.-Tidn. 1977;29:917–927.
    18. Whitaker J R. Determination of molecular weights of proteins by gel filtration on Sephadex. Analyt. Chem. 1963;35:1950–1953.
      doi: 10.1021/ac60205a048google scholar: lookup
    19. Yokohama M, Kuwasima M, Miura N, Snoue M, Mogi K, Hosoda T, Nakajima T. On normality of equine transferrin contents in general pupulation and quantitative difference of transferrin protein of normal homozygote and heterozygote with silent gene. Jap. J. zootech. Sci. 1980;51:336–341.

    Citations

    This article has been cited 1 times.
    1. Ek N. Concentration of serum transferrin in sick horses and its relationship to serum albumin content. Acta Vet Scand 1981;22(2):260-71.
      doi: 10.1186/BF03547515pubmed: 7304366google scholar: lookup
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