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Home / Equine Research Bank / Sci Rep / Antigen array for serological diagnosis and novel allergen i...
Scientific reports2019; 9(1); 15170; doi: 10.1038/s41598-019-51820-7

Antigen array for serological diagnosis and novel allergen identification in severe equine asthma.

Abstract: Severe equine asthma (sEA), which closely resembles human asthma, is a debilitating and performance-limiting allergic respiratory disorder which affects 14% of horses in the Northern Hemisphere and is associated with increased allergen-specific immunoglobulin E (IgE) against a range of environmental proteins. A comprehensive microarray platform was developed to enable the simultaneous detection of allergen-specific equine IgE in serum against a wide range of putative allergenic proteins. The microarray revealed a plethora of novel pollen, bacteria, mould and arthropod proteins significant in the aetiology of sEA. Moreover, the analyses revealed an association between sEA-affected horses and IgE antibodies specific for proteins derived from latex, which has traditionally been ubiquitous to the horse's environment in the form of riding surfaces and race tracks. Further work is required to establish the involvement of latex proteins in sEA as a potential risk factor. This work demonstrates a novel and rapid approach to sEA diagnosis, providing a platform for tailored management and the development of allergen-specific immunotherapy.
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Publication Date: 2019-10-23 PubMed ID: 31645629PubMed Central: PMC6811683DOI: 10.1038/s41598-019-51820-7Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper is about the use of a new microarray platform to detect allergens and provide diagnosis in horses suffering from severe equine asthma, a condition similar to human asthma. The study also identified a potential risk factor – proteins derived from latex – for further investigation.

Understanding Severe Equine Asthma (sEA)

  • Severe Equine Asthma (sEA) is an allergic respiratory condition in horses that is similar to human asthma.
  • Around 14% of horses in the Northern Hemisphere are affected by this performance-limiting disorder.
  • The condition is associated with an increase in allergen-specific Immunoglobulin E (IgE), which is triggered by various environmental proteins.

Development of the Microarray Platform

  • The research team developed a comprehensive microarray platform, a technology that allows for the simultaneous detection of a wide range of allergen-specific equine IgE in horse serum.
  • This platform was designed to easily identify a variety of potential allergens that could trigger allergic reactions in horses.

Identification of Novel Allergens & Association with Latex

  • Through the use of the microarray, the research team was able to identify a range of new proteins significant in causing sEA, including those derived from pollen, bacteria, mould, and arthropods.
  • Interestingly, the analyses also revealed a potential association between horses affected by sEA and IgE antibodies specific to proteins derived from latex.
  • Latex is present in horses’ environments in various forms, such as riding surfaces and race tracks.

Potential Role of Latex Proteins & Future Research

  • The findings highlighted the need for further research to establish whether latex proteins play a role in severe equine asthma, which could position them as a potential risk factor for the disorder.
  • Understanding this association could potentially help in the development of new prevention and treatment strategies for sEA.

Contribution of the Study

  • The research provides a fresh and swift approach to diagnosing sEA and could pave the way for a more personalised management of the condition.
  • Furthermore, the study’s findings could contribute to understanding the allergenic triggers of sEA, with implications for potential future development of allergen-specific immunotherapies.

Cite This Article

APA
White SJ, Moore-Colyer M, Marti E, Hannant D, Gerber V, Coüetil L, Richard EA, Alcocer M. (2019). Antigen array for serological diagnosis and novel allergen identification in severe equine asthma. Sci Rep, 9(1), 15170. https://doi.org/10.1038/s41598-019-51820-7

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 9
Issue: 1
Pages: 15170
PII: 15170

Researcher Affiliations

White, S J
  • Royal Agricultural University, Cirencester, Gloucestershire, GL7 6JS, UK. samuel.white@ntu.ac.uk.
  • School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK. samuel.white@ntu.ac.uk.
  • Nottingham Trent University, Brackenhurst Campus, Southwell, Nottinghamshire, NG25 0QF, UK. samuel.white@ntu.ac.uk.
Moore-Colyer, M
  • Royal Agricultural University, Cirencester, Gloucestershire, GL7 6JS, UK.
Marti, E
  • Department of Clinical Research and Veterinary Public Health, University of Bern, Bremgartenstr, Postfach, 3001, Bern, Switzerland.
Hannant, D
  • School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
Gerber, V
  • Department of Clinical Research and Veterinary Public Health, University of Bern, Bremgartenstr, Postfach, 3001, Bern, Switzerland.
Coüetil, L
  • Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, 47907, USA.
Richard, E A
  • LABÉO Frank Duncombe, 1 route de Rosel, 14053, Caen, Cedex 4, France.
  • Normandie Univ, UniCaen, BIOTARGEN, 3 rue Nelson Mandela, 14280, Saint-Contest, France.
Alcocer, M
  • School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.

MeSH Terms

  • Allergens / blood
  • Animals
  • Antigens / blood
  • Asthma / blood
  • Asthma / diagnosis
  • Asthma / veterinary
  • Discriminant Analysis
  • Horses / blood
  • Horses / immunology
  • Least-Squares Analysis
  • Serologic Tests

Conflict of Interest Statement

The authors declare no competing interests.

References

This article includes 51 references
  1. Hotchkiss JW, Reid SW, Christley RM. A survey of horse owners in Great Britain regarding horses in their care. Part 1: Horse demographic characteristics and management.. Equine Vet J 2007 Jul;39(4):294-300.
    doi: 10.2746/042516407X177538pubmed: 17722719google scholar: lookup
  2. Halliwell RE, McGorum BC, Irving P, Dixon PM. Local and systemic antibody production in horses affected with chronic obstructive pulmonary disease.. Vet Immunol Immunopathol 1993 Oct;38(3-4):201-15.
    doi: 10.1016/0165-2427(93)90081-Epubmed: 8291200google scholar: lookup
  3. Schmallenbach KH, Rahman I, Sasse HH, Dixon PM, Halliwell RE, McGorum BC, Crameri R, Miller HR. Studies on pulmonary and systemic Aspergillus fumigatus-specific IgE and IgG antibodies in horses affected with chronic obstructive pulmonary disease (COPD).. Vet Immunol Immunopathol 1998 Dec 11;66(3-4):245-56.
    doi: 10.1016/S0165-2427(98)00202-5pubmed: 9880102google scholar: lookup
  4. Gerber H, Hockenjos P, Lazary S, Kings M, de Weck A. [Histamine release from equine leucocytes provoked by fungal allergens].. Dtsch Tierarztl Wochenschr 1982 Jul 6;89(7):267-70.
    pubmed: 6181953
  5. Dirscherl P, Grabner A, Buschmann H. Responsiveness of basophil granulocytes of horses suffering from chronic obstructive pulmonary disease to various allergens.. Vet Immunol Immunopathol 1993 Oct;38(3-4):217-27.
    doi: 10.1016/0165-2427(93)90082-Fpubmed: 7507274google scholar: lookup
  6. Hare JE, Viel L, Conlon PD, Marshall JS. In vitro allergen-induced degranulation of pulmonary mast cells from horses with recurrent airway obstruction (heaves).. Am J Vet Res 1999 Jul;60(7):841-7.
    pubmed: 10407477
  7. Marti E, Wang X, Jambari NN, Rhyner C, Olzhausen J, Pérez-Barea JJ, Figueredo GP, Alcocer MJ. Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity.. Vet Immunol Immunopathol 2015 Oct 15;167(3-4):171-7.
    doi: 10.1016/j.vetimm.2015.06.013pubmed: 26163936google scholar: lookup
  8. Eder C, Crameri R, Mayer C, Eicher R, Straub R, Gerber H, Lazary S, Marti E. Allergen-specific IgE levels against crude mould and storage mite extracts and recombinant mould allergens in sera from horses affected with chronic bronchitis.. Vet Immunol Immunopathol 2000 Mar 15;73(3-4):241-53.
    doi: 10.1016/S0165-2427(00)00154-9pubmed: 10713338google scholar: lookup
  9. Couetil L, Kamarudin M, Aime M, HoganEsch H, Rosenthal F. Identification of fungal aeroallergens associated with recurrent airway obstruction in horses by immunoblotting. In Underlying Concepts In Chronic Lung Disease, From Injury To Dysregulated Repair (Veterinary Comparative Respiratory Society, 2015).
  10. Künzle F, Gerber V, Van Der Haegen A, Wampfler B, Straub R, Marti E. IgE-bearing cells in bronchoalveolar lavage fluid and allergen-specific IgE levels in sera from RAO-affected horses.. J Vet Med A Physiol Pathol Clin Med 2007 Feb;54(1):40-7.
    doi: 10.1111/j.1439-0442.2007.00870.xpubmed: 17359454google scholar: lookup
  11. Niedzwiedz A, Jaworski Z, Kubiak K. Serum concentrations of allergen-specific IgE in horses with equine recurrent airway obstruction and healthy controls assessed by ELISA.. Vet Clin Pathol 2015 Sep;44(3):391-6.
    doi: 10.1111/vcp.12274pubmed: 26175133google scholar: lookup
  12. White SJ. Development of a Multiple Protein Extract Microarray for Profiling Allergen-Specific Immunoglobulin E in Horses with Severe Asthma. In World Equine Airways Symposium107 (2017).
  13. Laumen E, Doherr MG, Gerber V. Relationship of horse owner assessed respiratory signs index to characteristics of recurrent airway obstruction in two Warmblood families.. Equine Vet J 2010 Mar;42(2):142-8.
    doi: 10.2746/042516409X479586pubmed: 20156250google scholar: lookup
  14. Tilley P, Sales Luis JP, Branco Ferreira M. Correlation and discriminant analysis between clinical, endoscopic, thoracic X-ray and bronchoalveolar lavage fluid cytology scores, for staging horses with recurrent airway obstruction (RAO).. Res Vet Sci 2012 Oct;93(2):1006-14.
    doi: 10.1016/j.rvsc.2011.10.024pubmed: 22136797google scholar: lookup
  15. Robinson NE. International Workshop on Equine Chronic Airway Disease. Michigan State University 16-18 June 2000.. Equine Vet J 2001 Jan;33(1):5-19.
    doi: 10.2746/042516401776767412pubmed: 11191611google scholar: lookup
  16. White S, Moore-Colyer M, Marti E, Coüetil L, Hannant D, Richard EA, Alcocer M. Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma.. J Vet Intern Med 2019 Sep;33(5):2327-2335.
    pmc: PMC6766494pubmed: 31429513doi: 10.1111/jvim.15564google scholar: lookup
  17. Verdon M, Lanz S, Rhyner C, Gerber V, Marti E. Allergen-specific immunoglobulin E in sera of horses affected with insect bite hypersensitivity, severe equine asthma or both conditions.. J Vet Intern Med 2019 Jan;33(1):266-274.
    pmc: PMC6335542pubmed: 30520523doi: 10.1111/jvim.15355google scholar: lookup
  18. Lee LC, Liong CY, Jemain AA. Partial least squares-discriminant analysis (PLS-DA) for classification of high-dimensional (HD) data: a review of contemporary practice strategies and knowledge gaps.. Analyst 2018 Jul 23;143(15):3526-3539.
    doi: 10.1039/C8AN00599Kpubmed: 29947623google scholar: lookup
  19. Adamko DJ, Nair P, Mayers I, Tsuyuki RT, Regush S, Rowe BH. Metabolomic profiling of asthma and chronic obstructive pulmonary disease: A pilot study differentiating diseases.. J Allergy Clin Immunol 2015 Sep;136(3):571-580.e3.
    doi: 10.1016/j.jaci.2015.05.022pubmed: 26152317google scholar: lookup
  20. Renault NK, Gaddipati SR, Wulfert F, Falcone FH, Mirotti L, Tighe PJ, Wright V, Alcocer MJ. Multiple protein extract microarray for profiling human food-specific immunoglobulins A, M, G and E.. J Immunol Methods 2011 Feb 1;364(1-2):21-32.
    doi: 10.1016/j.jim.2010.10.004pubmed: 20974146google scholar: lookup
  21. Nandy A, Creticos PS, Häfner D. Recombinant Allergens in Specific Immunotherapy. in Molecular Allergy Diagnostics 473–486 (Springer International Publishing, 2017).
    doi: 10.1007/978-3-319-42499-6_26google scholar: lookup
  22. Dutkiewicz J, Kus L, Dutkiewicz E, Warren CP. Hypersensitivity pneumonitis in grain farmers due to sensitization to Erwinia herbicola.. Ann Allergy 1985 Jan;54(1):65-8.
    pubmed: 3966692
  23. Roussel S, Reboux G, Rognon B, Monod M, Grenouillet F, Quadroni M, Fellrath JM, Aubert JD, Dalphin JC, Millon L. Comparison of three antigenic extracts of Eurotium amstelodami in serological diagnosis of farmer's lung disease.. Clin Vaccine Immunol 2010 Jan;17(1):160-7.
    doi: 10.1128/CVI.00129-09pmc: PMC2812093pubmed: 19906892google scholar: lookup
  24. Lugauskas A, Krikstaponis A, Sveistyte L. Airborne fungi in industrial environments--potential agents of respiratory diseases.. Ann Agric Environ Med 2004;11(1):19-25.
    pubmed: 15236494
  25. Kahn IS, Grothans EM. Hay fever and asthma due to red cedar (Juniperus Virginiana) and to mountain cedar (Juniperus Sabinoides). J. Allergy 1931;2:515.
  26. Milián E, Díaz AM. Allergy to house dust mites and asthma.. P R Health Sci J 2004 Mar;23(1):47-57.
    pubmed: 15125219
  27. Bessot JC, Metz-Favre C, Rame JM, De Blay F, Pauli G. Tropomyosin or not tropomyosin, what is the relevant allergen in house dust mite and snail cross allergies?. Eur Ann Allergy Clin Immunol 2010 Feb;42(1):3-10.
    pubmed: 20355359
  28. Uspenskaia K, Luss L, Babakhin A. Allergy to natural rubber latex in asthma patients. Clin. Transl. Allergy 2011;1:P65.
    doi: 10.1186/2045-7022-1-S1-P65google scholar: lookup
  29. Lühe T, Mielenz N, Schulz J, Dreyer-Rendelsmann C, Kemper N. Factors associated with dust dispersed in the air of indoor riding arenas.. Equine Vet J 2017 Jan;49(1):73-78.
    doi: 10.1111/evj.12528pubmed: 26517981google scholar: lookup
  30. Claußen G, Hessel EF. Particulate Matter in Equestrian Stables and Riding Arenas. J. Equine Vet. Sci. 2017;55:60–70.
    doi: 10.1016/j.jevs.2017.04.004google scholar: lookup
  31. Kollar JL, Swinker AM, Swinker ML, Irlbeck N. Dust Exposure and Respiratory Disorders in Equine Instructors. Prof. Anim. Sci. 2005;21:128–132.
    doi: 10.15232/S1080-7446(15)31181-5google scholar: lookup
  32. Donaldson K, Stone V, Gilmour PS, Brown DM, MacNee W. Ultrafine particles: Mechanisms of lung injury. Phil. Trans. R. Soc. Lond. A. 2000;358:2741–2749.
    doi: 10.1098/rsta.2000.0681google scholar: lookup
  33. Granum B, Gaarder PI, Løvik M. IgE adjuvant effect caused by particles - immediate and delayed effects.. Toxicology 2001 Jan 2;156(2-3):149-59.
    doi: 10.1016/S0300-483X(00)00375-9pubmed: 11164617google scholar: lookup
  34. Diaz-Sanchez D, Garcia MP, Wang M, Jyrala M, Saxon A. Nasal challenge with diesel exhaust particles can induce sensitization to a neoallergen in the human mucosa.. J Allergy Clin Immunol 1999 Dec;104(6):1183-8.
    doi: 10.1016/S0091-6749(99)70011-4pubmed: 10588999google scholar: lookup
  35. Granum B, Gaarder PI, Eikeset A, Stensby BA, Løvik M. The adjuvant effect of particles-importance of genetic background and pre-sensitisation.. Int Arch Allergy Immunol 2000 Jul;122(3):167-73.
    doi: 10.1159/000024393pubmed: 10899759google scholar: lookup
  36. Williams PB, Buhr MP, Weber RW, Volz MA, Koepke JW, Selner JC. Latex allergen in respirable particulate air pollution.. J Allergy Clin Immunol 1995 Jan;95(1 Pt 1):88-95.
    doi: 10.1016/S0091-6749(95)70156-7pubmed: 7822669google scholar: lookup
  37. Miguel AG, Cass GR, Weiss J, Glovsky MM. Latex allergens in tire dust and airborne particles.. Environ Health Perspect 1996 Nov;104(11):1180-6.
    doi: 10.1289/ehp.961041180pmc: PMC1469526pubmed: 8959407google scholar: lookup
  38. Hotchkiss JW, Reid SW, Christley RM. A survey of horse owners in Great Britain regarding horses in their care. Part 2: Risk factors for recurrent airway obstruction.. Equine Vet J 2007 Jul;39(4):301-8.
    doi: 10.2746/042516407X180129pubmed: 17722720google scholar: lookup
  39. Vandenplas O, Jamart J, Delwiche JP, Evrard G, Larbanois A. Occupational asthma caused by natural rubber latex: outcome according to cessation or reduction of exposure.. J Allergy Clin Immunol 2002 Jan;109(1):125-30.
    doi: 10.1067/mai.2002.120760pubmed: 11799378google scholar: lookup
  40. Yagami A, Suzuki K, Saito H, Matsunaga K. Hev B 6.02 is the most important allergen in health care workers sensitized occupationally by natural rubber latex gloves.. Allergol Int 2009 Sep;58(3):347-55.
    doi: 10.2332/allergolint.08-OA-0046pubmed: 19454841google scholar: lookup
  41. Raulf M, Quirce S, Vandenplas O. Addressing Molecular Diagnosis of Occupational Allergies.. Curr Allergy Asthma Rep 2018 Feb 14;18(1):6.
    doi: 10.1007/s11882-018-0759-9pmc: PMC5813088pubmed: 29445888google scholar: lookup
  42. Tahon L, Baselgia S, Gerber V, Doherr MG, Straub R, Robinson NE, Marti E. In vitro allergy tests compared to intradermal testing in horses with recurrent airway obstruction.. Vet Immunol Immunopathol 2009 Jan 15;127(1-2):85-93.
    doi: 10.1016/j.vetimm.2008.09.021pubmed: 19027178google scholar: lookup
  43. Pomés A, Chapman MD, Vailes LD, Blundell TL, Dhanaraj V. Cockroach allergen Bla g 2: structure, function, and implications for allergic sensitization.. Am J Respir Crit Care Med 2002 Feb 1;165(3):391-7.
    doi: 10.1164/ajrccm.165.3.2104027pubmed: 11818327google scholar: lookup
  44. Xi C, Sun B. IgE cross-reactivity between house dust mite and shrimp. in 5.3 Allergy and Immunology48, PA1092 (European Respiratory Society, 2016).
  45. Kehrli D, Jandova V, Fey K, Jahn P, Gerber V. Multiple hypersensitivities including recurrent airway obstruction, insect bite hypersensitivity, and urticaria in 2 warmblood horse populations.. J Vet Intern Med 2015 Jan;29(1):320-6.
    doi: 10.1111/jvim.12473pmc: PMC4858110pubmed: 25270534google scholar: lookup
  46. Lanz S, Brunner A, Graubner C, Marti E, Gerber V. Insect Bite Hypersensitivity in Horses is Associated with Airway Hyperreactivity.. J Vet Intern Med 2017 Nov;31(6):1877-1883.
    doi: 10.1111/jvim.14817pmc: PMC5697210pubmed: 28921663google scholar: lookup
  47. Morán G, Folch H, Araya O, Burgos R, Barria M. Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay.. Vet Res Commun 2010 Dec;34(8):719-26.
    doi: 10.1007/s11259-010-9440-zpubmed: 20814741google scholar: lookup
  48. McGorum BC, Dixon PM, Halliwell RE. Quantification of histamine in plasma and pulmonary fluids from horses with chronic obstructive pulmonary disease, before and after 'natural (hay and straw) challenges'.. Vet Immunol Immunopathol 1993 Apr;36(3):223-37.
    doi: 10.1016/0165-2427(93)90021-Upubmed: 8506613google scholar: lookup
  49. Pirie RS. Recurrent airway obstruction: a review.. Equine Vet J 2014 May;46(3):276-88.
    doi: 10.1111/evj.12204pubmed: 24164473google scholar: lookup
  50. Einhorn L, Hofstetter G, Brandt S, Hainisch EK, Fukuda I, Kusano K, Scheynius A, Mittermann I, Resch-Marat Y, Vrtala S, Valenta R, Marti E, Rhyner C, Crameri R, Satoh R, Teshima R, Tanaka A, Sato H, Matsuda H, Pali-Schöll I, Jensen-Jarolim E. Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer.. Allergy 2018 Jul;73(7):1436-1446.
    doi: 10.1111/all.13417pmc: PMC6032949pubmed: 29350763google scholar: lookup
  51. García BE, Lizaso MT. Cross-reactivity syndromes in food allergy.. J Investig Allergol Clin Immunol 2011;21(3):162-70; quiz 2 p following 170.
    pubmed: 21548443

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    doi: 10.1111/jvim.16600pubmed: 36479920google scholar: lookup
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