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Home / Equine Research Bank / Can J Comp Med / Antigenic analyses of tissues and excretory and secretory pr...
Canadian journal of comparative medicine : Revue canadienne de medecine comparee1981; 45(3); 259-265;

Antigenic analyses of tissues and excretory and secretory products from Strongylus vulgaris.

Abstract: Rabbit antisera were prepared against veronal buffered saline extracts of L4 and L5 Strongylus vulgaris, adult S. vulgaris and adult Strongylus equinus retrieved from naturally infected horses. In agar gel diffusion with these antisera, adult S vulgaris and S. equinus each appeared to have at least one unique antigen; larval S. vulgaris appeared to have two species-specific and two stage-specific antigens. There were several common antigens. Excretory and secretory products were collected also from L4 and L5 an maintained over several days in tissue culture fluid. In agar gel diffusion against the above rabbit antisera, a stage-specific antigen was found also in excretory and secretory products. In addition, excretory and secretory products had three antigens in common with adult and larval S. vulgaris, but only one of these was common to adult S. equinus. The excretory and secretory products appear, therefore, to have two species-specific and one stage-specific antigens.
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Publication Date: 1981-07-01 PubMed ID: 6804070PubMed Central: PMC1320217
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a scientific study on the antigenic analysis of tissues and the excretory and secretory products obtained from a type of parasitic worm, Strongylus vulgaris. The study aims to understand the unique and common antigens present in different stages of the parasite.

Methodology

  • The researchers prepared rabbit antisera using veronal buffered saline extracts of L4 and L5 (larval stages) of Strongylus vulgaris, as well as adult S. vulgaris and adult Strongylus equinus obtained from horses naturally infected with these parasites.
  • Using agar gel diffusion – a technique widely used to study the antigen-antibody reactions – they examined the produced antisera against different stages of the parasite (larvae, adults) and the excretory and secretory products collected from the larvae.

Findings

  • The agar gel diffusion test revealed that adult S. vulgaris and S. equinus parasites each had at least one unique antigen. The larval stage of S. vulgaris exhibited two species-specific antigens and two stage-specific antigens.
  • Additionally, the researchers observed several antigens common to different stages or species of the parasite.
  • The analysis of excretory and secretory products obtained from L4 and L5 larval stages revealed the presence of a stage-specific antigen. These products also exhibited three antigens that matched those found in adult and larval S. vulgaris. Among these three antigens, only one was also found in adult S. equinus.
  • This indicates that the excretory and secretory products of the larval stages contain two species-specific and one stage-specific antigens.

Conclusion

  • This research provides important insights into the antigenic differences and similarities in the different stages (larval and adult) of Strongylus vulgaris and between S. vulgaris and S. equinus. Understanding the antigenic profile of these parasites helps in studying their biology, pathogenesis, and might inform the development of potential therapeutic strategies

Cite This Article

APA
Wynne E, Slocombe JO, Wilkie BN. (1981). Antigenic analyses of tissues and excretory and secretory products from Strongylus vulgaris. Can J Comp Med, 45(3), 259-265.

Publication

Canadian journal of comparative medicine : Revue canadienne de medecine comparee
ISSN: 0008-4050
NlmUniqueID: 0151747
Country: Canada
Language: English
Volume: 45
Issue: 3
Pages: 259-265

Researcher Affiliations

Wynne, E
    Slocombe, J O
      Wilkie, B N

        MeSH Terms

        • Animals
        • Antigens / analysis
        • Antigens / immunology
        • Antigens / isolation & purification
        • Cecum / immunology
        • Hemagglutination Tests
        • Horses / immunology
        • Immunodiffusion
        • Strongyle Infections, Equine / immunology
        • Strongyloidea / immunology

        References

        This article includes 12 references
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        Citations

        This article has been cited 2 times.
        1. Andersen UV, Howe DK, Dangoudoubiyam S, Toft N, Reinemeyer CR, Lyons ET, Olsen SN, Monrad J, Nejsum P, Nielsen MK. SvSXP: a Strongylus vulgaris antigen with potential for prepatent diagnosis. Parasit Vectors 2013 Apr 4;6:84.
          doi: 10.1186/1756-3305-6-84pubmed: 23557195google scholar: lookup
        2. Bailey M, Lloyd S, Martin SC, Soulsby EJ. In vitro induction of lymphocyte responsiveness by a Strongylus vulgaris-derived mitogen. Z Parasitenkd 1984;70(2):229-42.
          doi: 10.1007/BF00942226pubmed: 6609495google scholar: lookup
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