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Home / Equine Research Bank / Arch Virol / Antigenic relatedness of equine herpes virus types 1 and 3.
Archives of virology1978; 56(1-2); 33-45; doi: 10.1007/BF01317281

Antigenic relatedness of equine herpes virus types 1 and 3.

Abstract: Antiserums prepared in specific pathogen free (SPF) ponies were used in direct and indirect immunofluorescence, immunodiffusion, complement fixation and serum neutralization procedures to study the interrelationships of the three types of equine herpes viruses (EHV-1, EHV-2, and EHV-3). Equine cell cultures infected with each type virus fluoresced when stained with homologous conjugated antiserum. In reciprocal tests EHV-1 and EHV-3 cross-fluoresced, but EHV-2 did not cross-fluoresce. Non-infected cell cultures did not fluoresce when stained with the 3 conjugates. EHV-1 and EHV-3 cross-fluoresced in reciprocal indirect fluorescent antibody tests, but no cross-fluorescence was shown with EHV-2. Antigens representing each type of equine herpes virus reacted with their homologous antiserum in the immunodiffusion test. In reciprocal tests, a common line(s) of identity formed with EHV-1 and EHV-3; however, the precipitin line(s) was not common with EHV-2. Antigen prepared from noninfected embryonic mule skin (EMS) cell cultures did not react with any of the antiserums. Specific complement-fixing antibodies were present in antiserums when tested against their homologous antigens. In reciprocal complement fixation tests EHV-1 and EHV-3 crossreacted, but no cross-reactivity was shown with EHV-2. Significant levels of neutralizing antibody were in an antiserum when tested against homologous virus, whereas cross-neutralization was not detectable in reciprocal tests. These studies indicate that each type of equine herpes virus contains specific antigenic components, and EHV-1 and EHV-3 share a common antigen(s) that is not shared with EHV-2.
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Publication Date: 1978-01-01 PubMed ID: 75724DOI: 10.1007/BF01317281Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explored the connection between equine herpes virus types 1 and 3, using various lab techniques to observe distinct antigenic components unique to each, while also revealing a shared common antigen between the two types.

Research Methodology and Procedures

  • The research utilized antiserums prepared in specific pathogen-free ponies to investigate the connections between the three types of equine herpes viruses (EHV – types 1, 2, and 3).
  • Various lab techniques such as direct and indirect immunofluorescence, immunodiffusion, complement fixation and serum neutralization procedures were employed to conduct this study.
  • Equine cell cultures infected with each type of virus were stained with a homologous conjugated antiserum. In reciprocal tests, EHV-1 and EHV-3 showed cross-fluorescence which means they shared common antigens, while EHV-2 did not.
  • Non-infected cell cultures were checked to assess whether they would fluoresce when stained with the 3 conjugates. They did not, providing a control for the experiment.

Findings and Results of the Study

  • Antigens representing each type of equine herpes virus reacted with their homologous antiserum in immunodiffusion tests. This indicates the specificity of the antigens for each type of herpes virus.
  • In reciprocal tests, EHV-1 and EHV-3 formed a common identity line, indicating shared antigens, but EHV-2 was different.
  • Specific complement-fixing antibodies were present in the antiserums tested against their respective antigens. However, EHV-1 and EHV-3 showed cross-reactivity, meaning shared antigens, whilst no cross-reactivity was detected for EHV-2.
  • Significant levels of neutralizing antibodies were found in an antiserum when it was tested against the homologous virus. On the other hand, cross-neutralization was not detectable in reciprocal tests, showing that the antibodies corresponding to each virus could not neutralize the other types.

Conclusion of the Study

  • It was concluded from the study that each type of equine herpes virus has its own distinct antigenic components, preserving their uniqueness.
  • The research also infers that EHV-1 and EHV-3 share a common antigen which is not found in EHV-2, displaying antigenic relatedness between EHV-1 and EHV-3 not shared with EHV-2.

Cite This Article

APA
Gutekunst DE, Malmquist WA, Becvar CS. (1978). Antigenic relatedness of equine herpes virus types 1 and 3. Arch Virol, 56(1-2), 33-45. https://doi.org/10.1007/BF01317281

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 56
Issue: 1-2
Pages: 33-45

Researcher Affiliations

Gutekunst, D E
    Malmquist, W A
      Becvar, C S

        MeSH Terms

        • Animals
        • Antibodies, Viral / analysis
        • Antigens, Viral / analysis
        • Complement Fixation Tests
        • Cross Reactions
        • Epitopes
        • Fluorescent Antibody Technique
        • Herpesviridae / immunology
        • Herpesvirus 1, Equid / immunology
        • Horses
        • Immunodiffusion
        • Neutralization Tests

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        Citations

        This article has been cited 2 times.
        1. Kirisawa R, Toishi Y, Akamatsu A, Soejima K, Miyashita T, Tsunoda N. Isolation of equine herpesvirus 3 (EHV-3) from equine coital exanthema of two stallions and sero-epidemiology of EHV-3 infection in Japan. J Vet Med Sci 2017 Mar 23;79(3):636-643.
          doi: 10.1292/jvms.16-0518pubmed: 28132964google scholar: lookup
        2. Kamada M, Studdert MJ. Analysis of small and large plaque variants of equine herpesvirus type 3 by restriction endonucleases. Brief report. Arch Virol 1983;77(2-4):259-64.
          doi: 10.1007/BF01309273pubmed: 6314939google scholar: lookup
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