Antigenic relationship between Pythium insidiosum de Cock et al. 1987 and its synonym Pythium destruens Shipton 1987.
Abstract: Antigens and rabbit-antisera from holotypes of Pythium insidiosum and P. destruens were prepared to elucidate their antigenic relationship. The antigens and rabbit-antisera of P. insidiosum as well as P. destruens used as a reference system showed that both shared three precipitin bands in common. The antigen and rabbit-antisera of P. destruens and P. insidiosum used as a reference system against other strains isolated from humans and animals with pythiosis, also showed three precipitin bands in common. When we used sera taken from horses with proven pythiosis against antigens of P. insidiosum and P. destruens, six common bands were observed. We concluded that the etiologic agent of pythiosis is a single species P. insidiosum, and could be identified by serologic methods.
Publication Date: 1989-02-01 PubMed ID: 2496306DOI: 10.1111/j.1439-0507.1989.tb02205.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research investigates the antigenic relationship between two species of Pythium, P. insidiosum and P. destruens, by examining common antigenic bands, in an effort to verify that they are a single species and can be identified through serologic methods.
Introduction
- This study was focused on elucidating the antigenic relationship between Pythium insidiosum and P. destruens, both of which are thought to be the same species.
- To achieve this, antigens and rabbit-antisera were prepared from the holotypes (original specimens) of P. insidiosum and P. destruens.
- The goal was to identify shared antigenic features, as confirmation of their being a single species.
Methodology
- Using the prepared antigen and rabbit-antisera from the holotypes, the researchers compared the antigenic characteristics of P. insidiosum and P. destruens.
- They used a reference system to observe how many precipitin bands (indicating antigen-antibody reactions) the two share.
- This comparison was also applied against other strains obtained from humans and animals infected with pythiosis (a disease caused by these organisms).
- Furthermore, they deployed sera derived from horses proven to suffer from pythiosis against antigens from both species.
Results
- Insights into the antigenic relationship were revealed by the shared precipitin bands.
- There were three shared bands found between the antigens and rabbit-antisera of both P. insidiosum and P. destruens.
- This pattern was mirrored when compared against other strains of pythiosis.
- Utilizing sera taken from horses with proven pythiosis, six common bands were identified between P. insidiosum and P. destruens.
Conclusion
- The research concludes that P. insidiosum and P. destruens are indeed the same species, serving as the etiologic agent (causative organism) of pythiosis.
- Opening up new perspectives for research and diagnostics, the authors suggest that this single species can be identified using serologic methods.
Cite This Article
APA
Mendoza L, Marin G.
(1989).
Antigenic relationship between Pythium insidiosum de Cock et al. 1987 and its synonym Pythium destruens Shipton 1987.
Mycoses, 32(2), 73-77.
https://doi.org/10.1111/j.1439-0507.1989.tb02205.x Publication
Researcher Affiliations
MeSH Terms
- Animals
- Antigens, Fungal / analysis
- Chytridiomycota / immunology
- Humans
- Immunodiffusion
- Mycoses / microbiology
- Pythium / immunology
Citations
This article has been cited 6 times.- Yolanda H, Krajaejun T. Global Distribution and Clinical Features of Pythiosis in Humans and Animals. J Fungi (Basel) 2022 Feb 11;8(2).
- Krajaejun T, Kittichotirat W, Patumcharoenpol P, Rujirawat T, Lohnoo T, Yingyong W. Draft genome sequence of the oomycete Pythium destruens strain ATCC 64221 from a horse with pythiosis in Australia. BMC Res Notes 2020 Jul 9;13(1):329.
- Salas Y, Márquez A, Canelón J, Perazzo Y, Colmenárez V, López JA. Equine pythiosis: report in crossed bred (Criole Venezuelan) horses. Mycopathologia 2012 Dec;174(5-6):511-7.
- Schurko AM, Mendoza L, de Cock AW, Bedard JE, Klassen GR. Development of a species-specific probe for Pythium insidiosum and the diagnosis of pythiosis. J Clin Microbiol 2004 Jun;42(6):2411-8.
- Mendoza L, Nicholson V, Prescott JF. Immunoblot analysis of the humoral immune response to Pythium insidiosum in horses with pythiosis. J Clin Microbiol 1992 Nov;30(11):2980-3.
- Mendoza L, Villalobos J, Calleja CE, Solis A. Evaluation of two vaccines for the treatment of pythiosis insidiosi in horses. Mycopathologia 1992 Aug;119(2):89-95.
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