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Home / Equine Research Bank / Infect Immun / Antigenic stimulation of T lymphocytes in chronic nononcogen...
Infection and immunity1982; 36(1); 38-46; doi: 10.1128/iai.36.1.38-46.1982

Antigenic stimulation of T lymphocytes in chronic nononcogenic retrovirus infection: equine infectious anemia.

Abstract: Equine infectious anemia is a chronic disease of horses caused by a nononcogenic retrovirus. Studies were undertaken to determine the types of cells involved in the in vitro lymphoproliferative response to viral antigens and the dynamics of this reaction. It was observed that reactive lymphocytes were present at unpredictable times in the peripheral blood of infected horses. This reaction was shown to be specific for the interaction of equine infectious anemia virus and T lymphocytes. Enriched B-lymphocyte populations did not divide when exposed to equine infectious anemia virus. Macrophages were depleted from the reaction by two methods: adherence to Sephadex and a combination of binding to Sephadex and adherence to complement-coated erythrocytes. Both methods reduced the number of monocytes, but only the combination of Sephadex and complement-coated cells removed the accessory cells needed for lymphocyte proliferation. We conclude that during the chronic stages of equine infectious anemia the number of antigen-reactive T lymphocytes fluctuates within the peripheral blood and that these cells require a complement-binding cell for reaction. The relationship of these cells to the lymphoproliferative stages of this disease is discussed.
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Publication Date: 1982-04-01 PubMed ID: 6281191PubMed Central: PMC351181DOI: 10.1128/iai.36.1.38-46.1982Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the types of cells involved in the immune response to equine infectious anemia, a chronic viral disease of horses, and how this response changes over time. The study found that T lymphocytes are key in the immune response to the virus, and the number of these cells in the blood of infected horses fluctuates unpredictably during the chronic stages of the disease.

Study Overview

  • The primary aim of this research was to determine the types of cells that are involved in the body’s immune response to equine infectious anemia virus (EIAV) and the dynamics of this response.
  • The researchers conducted their study using in vitro methods where conditions could be controlled more efficiently.

Key Findings

  • The researchers found out that T lymphocytes, a type of white blood cell, were involved in the immune response to EIAV.
  • Meanwhile, another type of lymphocytes, B lymphocytes, did not respond to the EIAV exposure.
  • Interestingly, the researchers also observed that the number of immune cells responding to the virus in the blood of infected horses was varying unpredictably over time.

Role of Macrophages

  • Macrophages, another type of immune cells, were found to play a role in lymphocyte activation.
  • When macrophages were removed from the response through processes such as adherence to Sephadex and complement-coated cells, there was a decline in lymphocyte proliferation, indicating their role in immune response.

Conclusions

  • The study concluded that T lymphocytes, specifically those that react with EIAV, increase and decrease unpredictably in the bloodstream during the chronic stages of equine infectious anemia.
  • The researchers also determined that these antigen-reactive T lymphocytes require the presence of another cell type, a complement-binding cell, in order to react with the virus.
  • This study contributes to broadening the understanding of disease mechanisms in equine infectious anemia and potentially facilitating the development of more effective treatments.

Cite This Article

APA
Shively MA, Banks KL, Greenlee A, Klevjer-Anderson P. (1982). Antigenic stimulation of T lymphocytes in chronic nononcogenic retrovirus infection: equine infectious anemia. Infect Immun, 36(1), 38-46. https://doi.org/10.1128/iai.36.1.38-46.1982

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 36
Issue: 1
Pages: 38-46

Researcher Affiliations

Shively, M A
    Banks, K L
      Greenlee, A
        Klevjer-Anderson, P

          MeSH Terms

          • Animals
          • Antigens, Viral / immunology
          • B-Lymphocytes / immunology
          • Cell Separation
          • Equine Infectious Anemia / immunology
          • Horses
          • Infectious Anemia Virus, Equine / immunology
          • Lectins / pharmacology
          • Lymphocyte Activation
          • Monocytes / immunology
          • T-Lymphocytes / immunology

          Grant Funding

          • AI07471 / NIAID NIH HHS
          • RR 05465-7 / NCRR NIH HHS

          References

          This article includes 34 references
          1. Mogensen CE. The glomerular permeability determined by dextran clearance using Sephadex gel filtration.. Scand J Clin Lab Invest 1968;21(1):77-82.
            pubmed: 5637478doi: 10.3109/00365516809076979google scholar: lookup
          2. Squire RA. Equine infectious anemia: a model of immunoproliferative disease.. Blood 1968 Jul;32(1):157-69.
            pubmed: 5690595
          3. Coggins L, Norcross NL. Immunodiffusion reaction in equine infectious anemia.. Cornell Vet 1970 Apr;60(2):330-5.
            pubmed: 4986043
          4. McGuire TC, Crawford TB, Henson JB. Immunofluorescent localization of equine infectious anemia virus in tissue.. Am J Pathol 1971 Feb;62(2):283-94.
            pubmed: 4322275
          5. Henson JB, McGuire TC. Immunopathology of equine infectious anemia.. Am J Clin Pathol 1971 Sep;56(3):306-13.
            pubmed: 4328671doi: 10.1093/ajcp/56.3.306google scholar: lookup
          6. Banks KL, Henson JB, McGuire TC. Immunologically mediated glomerulitis of horses. I. Pathogenesis in persistent infection by equine infectious anemia virus.. Lab Invest 1972 Jun;26(6):701-7.
            pubmed: 4337973
          7. Ushimi C, Henson JB, Gorham JR. Study of the one-step growth curve of equine infectious anemia virus by immunofluorescence.. Infect Immun 1972 Jun;5(6):890-5.
            pubmed: 4344094doi: 10.1128/iai.5.6.890-895.1972google scholar: lookup
          8. Kono Y, Kobayashi K, Fukunaga Y. Antigenic drift of equine infectious anemia virus in chronically infected horses.. Arch Gesamte Virusforsch 1973;41(1):1-10.
            pubmed: 4123810doi: 10.1007/BF01249923google scholar: lookup
          9. Banks KL, Henson JB. Quantitation of immunoglobulin-bearing lymphocytes and lymphocyte response to mitogens in horses persistently infected by equine infectious anemia virus.. Infect Immun 1973 Oct;8(4):679-82.
            pubmed: 4582640doi: 10.1128/iai.8.4.679-682.1973google scholar: lookup
          10. Malmquist WA, Barnett D, Becvar CS. Production of equine infectious anemia antigen in a persistently infected cell line.. Arch Gesamte Virusforsch 1973;42(4):361-70.
            pubmed: 4358259doi: 10.1007/BF01250717google scholar: lookup
          11. Ly IA, Mishell RI. Separation of mouse spleen cells by passage through columns of sephadex G-10.. J Immunol Methods 1974 Aug;5(3):239-47.
            pubmed: 4610054doi: 10.1016/0022-1759(74)90108-2google scholar: lookup
          12. Banks KL, McGuire TC. Surface receptors on neutrophils and monocytes from immunodeficient and normal horses.. Immunology 1975 Mar;28(3):581-8.
            pubmed: 1126740
          13. Ishii S, Ishitani R. Equine infectious anemia.. Adv Vet Sci Comp Med 1975;19:195-222.
            pubmed: 174411
          14. McGuire TC. Suppression of synthesis of an IgG subclass in a persistent viral infection.. Immunology 1976 Jan;30(1):17-24.
            pubmed: 814077
          15. Lee JC, Ihle JN. Characterization of the blastogenic and cytotoxic responses of normal mice to ecotropic C-type viral gp71.. J Immunol 1977 Mar;118(3):928-34.
            pubmed: 191530
          16. Levy JP, Leclerc JC. The murine sarcoma virus-induced tumor: exception or general model in tumor immunology?. Adv Cancer Res 1977;24:1-66.
            pubmed: 66859doi: 10.1016/s0065-230x(08)61012-xgoogle scholar: lookup
          17. Cheevers WP, Archer BG, Crawford TB. Characterization of RNA from equine infectious anemia virus.. J Virol 1977 Nov;24(2):489-97.
            pubmed: 199735doi: 10.1128/JVI.24.2.489-497.1977google scholar: lookup
          18. Osbaldiston GW, Sullivan RJ. Cytochemical demonstration of esterases in peripheral blood leukocytes.. Am J Vet Res 1978 Apr;39(4):683-5.
            pubmed: 25603
          19. Griffin DE, Narayan O, Adams RJ. Early immune responses in visna, a slow viral disease of sheep.. J Infect Dis 1978 Sep;138(3):340-50.
            pubmed: 212487doi: 10.1093/infdis/138.3.340google scholar: lookup
          20. Izui S, McConahey PJ, Theofilopoulos AN, Dixon FJ. Association of circulating retroviral gp70-anti-gp70 immune complexes with murine systemic lupus erythematosus.. J Exp Med 1979 May 1;149(5):1099-116.
            pubmed: 221610doi: 10.1084/jem.149.5.1099google scholar: lookup
          21. McGrath MS, Weissman IL. AKR leukemogenesis: identification and biological significance of thymic lymphoma receptors for AKR retroviruses.. Cell 1979 May;17(1):65-75.
            pubmed: 222476doi: 10.1016/0092-8674(79)90295-2google scholar: lookup
          22. Fujimiya Y, Perryman LE, Crawford TB. Leukocyte cytotoxicity in a persistent virus infection: presence of direct cytotoxicity but absence of antibody-dependent cellular cytotoxicity in horses infected with equine infectious anemia virus.. Infect Immun 1979 Jun;24(3):628-36.
            pubmed: 223981doi: 10.1128/iai.24.3.628-636.1979google scholar: lookup
          23. Diamantstein T, Oppenheim JJ, Unanue ER, Wood DD, Handschumacher RE, Rosenstreich DL, Waksman BH. Nonspecific "lymphocyte activating" factors produced by macrophages.. Clin Immunol Immunopathol 1979 Oct;14(2):264-7.
            pubmed: 226303doi: 10.1016/0090-1229(79)90149-1google scholar: lookup
          24. Lee JC, Ihle JN. Mechanisms of C-type viral leukemogenesis. I. Correlation of in vitro lymphocyte blastogenesis to viremia and leukemia.. J Immunol 1979 Nov;123(5):2351-8.
            pubmed: 90708
          25. Feldmann M, Rosenthal A, Erb P. Macrophage-lymphocyte interactions in immune induction.. Int Rev Cytol 1979;60:149-78.
            pubmed: 115808doi: 10.1016/s0074-7696(08)61262-0google scholar: lookup
          26. Klevjer-Anderson P, Cheevers WP, Crawford TB. Characterization of the infection of equine fibroblasts by equine infectious anemia virus.. Arch Virol 1979;60(3-4):279-89.
            pubmed: 228638doi: 10.1007/BF01317499google scholar: lookup
          27. Bubbers JE, Elder JH, Dixon FJ. Stimulation of murine lymphocytes by Rauscher leukemia virus in vitro.. J Immunol 1980 Jan;124(1):388-94.
            pubmed: 7350233
          28. McGrath MS, Pillemer E, Weissman IL. Murine leukaemogenesis: monoclonal antibodies to T-cell determinants arrest T-lymphoma cell proliferation.. Nature 1980 May 22;285(5762):259-61.
            pubmed: 6246448doi: 10.1038/285259a0google scholar: lookup
          29. Adams DS, Crawford TB, Banks KL, McGuire TC, Perryman LE. Immune responses of goats persistently infected with caprine arthritis-encephalitis virus.. Infect Immun 1980 May;28(2):421-7.
            pubmed: 7399671doi: 10.1128/iai.28.2.421-427.1980google scholar: lookup
          30. McGuire TC, Crawford TB. Immunology of a persistent retrovirus infection--equine infectious anemia.. Adv Vet Sci Comp Med 1979;23:137-59.
            pubmed: 95154
          31. Lee JC, Horak I, Ihle JN. Mechanisms in T cell leukemogenesis. II. T cell responses of preleukemic BALB/c mice to Moloney leukemia virus antigens.. J Immunol 1981 Feb;126(2):715-22.
            pubmed: 6969759
          32. Enjuanes L, Lee JC, Ihle JN. T cell recognition of Moloney sarcoma virus proteins during tumor regression. I. Lack of a requirement for macrophages and the role of blastogenic factors in T cell proliferation.. J Immunol 1981 Apr;126(4):1478-84.
            pubmed: 6970777
          33. Sihvonen L. Early immune responses in experimental maedi.. Res Vet Sci 1981 Mar;30(2):217-22.
            pubmed: 6265996
          34. Banks KL, Greenlee A. Isolation and identification of equine lymphocytes and monocytes.. Am J Vet Res 1981 Sep;42(9):1651-4.
            pubmed: 6976768

          Citations

          This article has been cited 4 times.
          1. Sellon DC, Fuller FJ, McGuire TC. The immunopathogenesis of equine infectious anemia virus.. Virus Res 1994 May;32(2):111-38.
            doi: 10.1016/0168-1702(94)90038-8pubmed: 8067050google scholar: lookup
          2. Johnson GC, Adams DS, McGuire TC. Pronounced production of polyclonal immunoglobulin G1 in the synovial fluid of goats with caprine arthritis-encephalitis virus infection.. Infect Immun 1983 Aug;41(2):805-15.
            doi: 10.1128/iai.41.2.805-815.1983pubmed: 6307882google scholar: lookup
          3. Valpotić I, Kastelan M, Rudolf M, Gerencer M, Jukić B, Basić I. T and B lymphocytes in horses persistently infected with equine infectious anaemia virus.. Vet Res Commun 1989;13(1):57-65.
            doi: 10.1007/BF00366853pubmed: 2788955google scholar: lookup
          4. Issel CJ, Horohov DW, Lea DF, Adams WV Jr, Hagius SD, McManus JM, Allison AC, Montelaro RC. Efficacy of inactivated whole-virus and subunit vaccines in preventing infection and disease caused by equine infectious anemia virus.. J Virol 1992 Jun;66(6):3398-408.
            doi: 10.1128/JVI.66.6.3398-3408.1992pubmed: 1316455google scholar: lookup
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