Antimicrobial Activity of Ceftiofur and Penicillin With Gentamicin Against Escherichia coli and Streptococcus equi Subspecies zooepidemicus in an Ex Vivo Model of Equine Postpartum Uterine Disease.
Abstract: The use of antimicrobials for the management of equine uterine disease is commonplace, with antibiotic selection generally based on empirical evidence or in vitro sensitivity results. However, the potential disconnect between these laboratory results and clinical efficacy in the mare raises concern for antibiotic failure and subsequent development of resistant organisms. In this work, we attempt to bridge this gap by using an ex vivo model of the equine postpartum uterus to quantitatively evaluate the antimicrobial activity of two commonly used antibiotic treatments in the mare (ceftiofur and penicillin with gentamicin). The activity of both of these treatments was evaluated in two different fluid environments (standard bacterial culture broth and equine postpartum uterine fluid) against clinical isolates of E. coli and S. zooepidemicus. Although treatment with ceftiofur was effective at reducing growth of S. zooepidemicus in equine postpartum uterine fluid, it did not reduce bacterial growth of E. coli. Treatment with procaine penicillin G with gentamicin achieved at least bacteriostatic activity against E. coli in both fluid types, and bactericidal activity against S. zooepidemicus in both fluid types. The intrauterine infusion of procaine penicillin G with gentamicin in cases of postpartum uterine disease caused by E. coli or S. zooepidemicus is supported by the results of this work.
Copyright © 2019 Elsevier Inc. All rights reserved.
Publication Date: 2019-06-20 PubMed ID: 31405490DOI: 10.1016/j.jevs.2019.06.005Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article investigates the effectiveness of two commonly used antibiotics, ceftiofur and a combination of penicillin with gentamicin, against pathogens causing postpartum uterine disease in horses.
Objective of the Research
- The study aimed to evaluate the antimicrobial activity of commonly used drugs, namely ceftiofur and the combination of penicillin with gentamicin, using an ex vivo model of the equine postpartum uterus.
- With the increasing concern over antibiotic failure and the subsequent development of resistant organisms, the scientists hoped to address the disconnect between laboratory results and clinical efficacy.
- The effectiveness of these treatments was tested in two different environments: standard bacterial culture broth and equine postpartum uterine fluid.
The Methodology
- The researchers conducted experiments on clinical isolates of Escherichia coli and Streptococcus equi subspecies zooepidemicus in both aforementioned fluid environments.
- They quantitatively evaluated the antimicrobial activity of the antibiotic treatments against these bacteria.
Findings of the Study
- Results showed that treatment with ceftiofur effectively reduced the growth of S. zooepidemicus in equine postpartum uterine fluid. However, this treatment did not effectively reduce the bacterial growth of E. coli.
- On the other hand, the treatment using procaine penicillin G with gentamicin showed bacteriostatic activity against E. coli in both fluid environments, and also displayed a bactericidal activity against S. zooepidemicus.
Significance of the Research
- The research supports the use of intrauterine infusion of procaine penicillin G with gentamicin in treating postpartum uterine disease caused by E. coli or S. zooepidemicus.
- These findings have significant implications for the treatment of equine uterine disease, helping veterinarians make more informed decisions regarding antibiotic treatment.
Cite This Article
APA
Von Dollen KA, Jones M, Beachler T, Harris TL, Papich MG, Lyle SK, Bailey CS.
(2019).
Antimicrobial Activity of Ceftiofur and Penicillin With Gentamicin Against Escherichia coli and Streptococcus equi Subspecies zooepidemicus in an Ex Vivo Model of Equine Postpartum Uterine Disease.
J Equine Vet Sci, 79, 121-126.
https://doi.org/10.1016/j.jevs.2019.06.005 Publication
Researcher Affiliations
- Departments of Clinical Sciences, North Carolina State University, Raleigh, NC.
- Departments of Clinical Sciences, North Carolina State University, Raleigh, NC.
- Departments of Clinical Sciences, North Carolina State University, Raleigh, NC.
- Departments of Clinical Sciences, North Carolina State University, Raleigh, NC.
- Molecular and Biomedical Sciences, North Carolina State University, Raleigh, NC.
- Departments of Clinical Sciences, North Carolina State University, Raleigh, NC.
- Departments of Clinical Sciences, North Carolina State University, Raleigh, NC. Electronic address: csbailey@ncsu.edu.
MeSH Terms
- Animals
- Anti-Bacterial Agents / therapeutic use
- Cephalosporins
- Drug Resistance, Bacterial
- Escherichia coli
- Female
- Gentamicins
- Horse Diseases / drug therapy
- Horses
- Humans
- Penicillins
- Postpartum Period
- Streptococcus equi
- Uterine Diseases / drug therapy
- Uterine Diseases / veterinary
Citations
This article has been cited 0 times.Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
