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Home / Equine Research Bank / Vet Med Int / Antimicrobial Activity of Plasma Rich in Platelets (PRP) on ...
Veterinary medicine international2023; 2023; 2407768; doi: 10.1155/2023/2407768

Antimicrobial Activity of Plasma Rich in Platelets (PRP) on the Ocular Microbiota of Healthy Horses from Minas Gerais: In Vitro Study.

Abstract: In equine ophthalmology, ulcerative keratitis is among the most common conditions and, in general, arises as a consequence of some trauma suffered. Secondarily, subsequent contamination by pathogenic or resident bacteria of the horse's ocular microbiota may have undesirable consequences. Under physiological conditions, the normal microbiota coexists with the immune status of the host, serving as a barrier, ensuring the health of the ocular surface, and inhibiting the proliferation of pathogens. However, in the imbalance of immune barriers, the normal microbiota can become pathogenic and lead to infection, acting as an opportunistic agent. The present study aims to demonstrate the antimicrobial effect of platelet-rich plasma (PRP), its time of action, and its correlation with the concentration of its same components in vitro on Staphylococcus sciuri, a bacterium with high prevalence in the normal ocular microbiota of horses in the municipality of Minas Gerais. For the preparation of the PRP, eight adult Quarter Horse (QH) horses were used. The individual PRP was prepared by the double centrifugation protocol, and then, the PRPs were added to a pool, followed by testing their interaction in culture with Brain Heart Infusion (BHI) broth at different dilutions against five strains collected from different animals. After 3, 6, 12, and 18 hours, the colony formation units (CFU) count on a 5% horse blood agar plate was evaluated for each time point. Our study showed that Staphylococcus sciuri, the resident microorganism of the ocular conjunctival microbiota of horses, is more susceptible when compared to the standard strain "American Type Culture Collection" (ATCC-29213) Staphylococcus aureus, a pathogenic microorganism, which was used for the validation of our study. The antibacterial effect shown in this study was bacteriostatic for up to 6 hours. The most concentrated PRP dilutions, 1 : 1 and 1 : 2, were also most effective, suggesting that the antibacterial effect is volume dependent.
Copyright © 2023 Luis E. C. Torres et al.
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Publication Date: 2023-08-16 PubMed ID: 37622165PubMed Central: PMC10447163DOI: 10.1155/2023/2407768Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the antimicrobial effect of Platelet-Rich Plasma (PRP) on bacteria commonly found in the eyes of horses to examine its potential in treating equine eye infections. The study documents PRP’s inhibitory effect on bacteria growth and its dependency on its concentration.

Introduction and Objective

  • The research was conducted against the backdrop of common eye infection conditions in horses, such as ulcerative keratitis often caused by trauma and subsequently contaminated by pathogenic bacteria.
  • The focus of the study was to understand the antimicrobial impact of Platelet-Rich Plasma (PRP) on the ocular bacteria of healthy horses, and how this might shed light on its utility for managing equine eye infections.

Methodology

  • Eight adult Quarter Horse (QH) horses were used to prepare the individual PRP using a double centrifugation protocol.
  • The PRPs were pooled and tested for their interaction with Brain Heart Infusion (BHI) broth on five different bacterial strains collected from different animals.
  • The study involved testing PRP’s impact on bacterial growth at varying dilutions and time intervals (3, 6, 12, and 18 hours). Colony formation units (CFU) count on a 5% horse blood agar was used as the evaluation measure.

Findings

  • The study discovered that a resident microorganism of the horse’s ocular microbiota is more vulnerable in the face of PRP as compared to a pathogenic microorganism used as the standard strain for comparative study.
  • The antibacterial effect of PRP was found to be bacteriostatic (inhibiting bacterial growth rather than killing bacteria) for up to 6 hours.
  • It was also identified that the most concentrated PRP dilutions (1:1 and 1:2) demonstrated the greatest effectiveness, suggesting an association between the antibacterial effect and the volume of PRP.

Implications

  • This study accentuates the potential of PRP as a therapeutic agent for managing bacterial ocular infections in horses.
  • The findings provide important insights into PRP’s specific antimicrobial actions and the role of PRP concentration in mediating this effect.

Cite This Article

APA
Torres LEC, Florez CO, Oliveira JG, Vieira GD, Ribeiro IS, Keller KM, Leme FOP, Fantini P, Maranhão RPA. (2023). Antimicrobial Activity of Plasma Rich in Platelets (PRP) on the Ocular Microbiota of Healthy Horses from Minas Gerais: In Vitro Study. Vet Med Int, 2023, 2407768. https://doi.org/10.1155/2023/2407768

Publication

Veterinary medicine international
ISSN: 2090-8113
NlmUniqueID: 101524203
Country: United States
Language: English
Volume: 2023
Pages: 2407768

Researcher Affiliations

Torres, Luis E C
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Florez, Camilo O
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Oliveira, Jéssica G
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Vieira, Giovanna D
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Ribeiro, Ilza S
  • Grupo UnieduK, Centro Universitário de Jaguariúna-UNIFAJ, Jaguariúna, São Paulo 13918-110, Brazil.
Keller, Kelly M
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Leme, Fabíola O P
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Fantini, Priscila
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Maranhão, Renata P A
  • Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.

Conflict of Interest Statement

The authors declare that there are no conflicts of interest.

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