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Veterinary research communications2009; 34(2); 133-141; doi: 10.1007/s11259-009-9338-9

Antioxidant capacity and oxidative damage determination in synovial fluid of chronically damaged equine metacarpophalangeal joint.

Abstract: In order to determine oxidative stress in equine joints with degenerative processes, we analyzed synovial fluid (SF) antioxidant capacity and the concentration of oxidative damage biomarkers in healthy and chronically damaged metacarpophalangeal joints. SF samples were collected from joints of thirty 2-5 year-old crossbreed male equine, macroscopically classified at post mortem inspection and later histologically confirmed. The antioxidant capacity was determined measuring uric acid and the concentration of sulfhydryl groups and the total radical trapping antioxidant potential (TRAP). The oxidative damage was determined by assessing malondialdehyde (MDA) and carbonyl protein concentration. TRAP was significantly higher (p < 0.05) in the group with chronic damage (CD). The sulfhydryl groups and concentration of uric acid did not show significant difference between the groups (p > 0.05). Although carbonyl concentration did not show significant difference between groups, it was slightly higher in the group with CD (p = 0.05009). Concentration of MDA did not show significant difference (p > 0.05) between groups. The observed significant increase in TRAP in the group with CD could be related to the participation of components other than protein, sulfhydryl groups, or uric acid coming from degenerating joint tissues. These findings could be helpful for a better understanding of the oxidative stress role in equine joints with chronic degenerative process.
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Publication Date: 2009-12-12 PubMed ID: 20012721DOI: 10.1007/s11259-009-9338-9Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers studied the oxidative stress in horse joints suffering from degeneration by assessing the antioxidant capacity and oxidative damage biomarkers in the synovial fluid of both healthy and chronically damaged joints. The results showed increased total radical trapping antioxidant potential in chronically damaged joints, which could be associated with degenerating joint tissues.

Objective and Method

  • The focus of the study was to determine the level of oxidative stress in horse joints undergoing a degenerative process.
  • The team analysed synovial fluid from both healthy and chronically damaged metacarpophalangeal joints in horses.
  • These joint fluid samples were taken from thirty 2-5 year-old cross-breed male horses post mortem, which were also subject to macroscopic classification and histological confirmation.

Assessment of Antioxidant Capacity

  • The antioxidant capacity of the fluid samples was determined via testing for uric acid and sulfhydryl group concentrations, as well as total radical trapping antioxidant potential (TRAP).

Evaluation of Oxidative Damage

  • The researchers deduced the level of oxidative damage by examining the concentration of malondialdehyde (MDA) and carbonyl proteins in the samples.

Findings

  • The TRAP value was significantly higher in the case of samples from chronically damaged (CD) joints, indicating more antioxidant capacity when chronic damage is present.
  • No significant difference was found in the availability of sulfhydryl groups and concentration of uric acid. However, a marginal increase in carbonyl protein concentration was observed in the CD group.
  • The concentration of MDA did not vary significantly across groups, indicating comparable oxidative damage.

Conclusions

  • The higher TRAP in CD groups could suggest that substances other than protein, sulfhydryl groups, or uric acid from degenerating joint tissues are inducing oxidative stress.
  • A clear understanding of this oxidative stress could lead to better comprehension of the degenerative process in equine joints and potentially improve future treatments.

Cite This Article

APA
Villasante A, Araneda OF, Behn C, Galleguillos M, Adarmes H. (2009). Antioxidant capacity and oxidative damage determination in synovial fluid of chronically damaged equine metacarpophalangeal joint. Vet Res Commun, 34(2), 133-141. https://doi.org/10.1007/s11259-009-9338-9

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 34
Issue: 2
Pages: 133-141

Researcher Affiliations

Villasante, Alejandro
  • Department of Biological Animal Sciences, Faculty of Veterinary Medicine, University of Chile, Santa Rosa 11.735, Santiago, Chile. vill0378@vandals.uidaho.edu
Araneda, Oscar F
    Behn, Claus
      Galleguillos, Marco
        Adarmes, Hector

          MeSH Terms

          • Animals
          • Antioxidants / metabolism
          • Horse Diseases / metabolism
          • Horses
          • Joint Diseases / metabolism
          • Joint Diseases / veterinary
          • Male
          • Malondialdehyde / metabolism
          • Oxidative Stress / physiology
          • Statistics, Nonparametric
          • Sulfhydryl Compounds / metabolism
          • Synovial Fluid / metabolism
          • Uric Acid / metabolism

          References

          This article includes 48 references
          1. Ann Clin Lab Sci. 2005 Winter;35(1):37-45
            pubmed: 15830708
          2. Free Radic Biol Med. 2007 Aug 15;43(4):477-503
            pubmed: 17640558
          3. Indian J Med Res. 2009 Feb;129(2):127-37
            pubmed: 19293438
          4. Vet J. 2006 Jan;171(1):51-69
            pubmed: 16427582
          5. Arthritis Res. 2001;3(2):107-13
            pubmed: 11178118
          6. Dis Markers. 2003-2004;19(1):27-31
            pubmed: 14757944
          7. Biol Res. 2000;33(2):65-70
            pubmed: 15693272
          8. Biochim Biophys Acta. 1986 Jan 30;869(2):119-27
            pubmed: 3942755
          9. Br Vet J. 1992 Sep-Oct;148(5):369-71
            pubmed: 1422780
          10. Free Radic Biol Med. 2004 Dec 1;37(11):1744-55
            pubmed: 15528034
          11. Crit Rev Toxicol. 1993;23(1):21-48
            pubmed: 8471159
          12. Nature. 2000 Nov 9;408(6809):239-47
            pubmed: 11089981
          13. Equine Vet J. 2003 Nov;35(7):697-701
            pubmed: 14649362
          14. Poult Sci. 2005 May;84(5):782-8
            pubmed: 15913191
          15. Free Radic Biol Med. 2008 Nov 1;45(9):1205-16
            pubmed: 18762244
          16. Biochim Biophys Acta. 1985 Feb 15;838(2):221-8
            pubmed: 3838255
          17. Ecotoxicol Environ Saf. 2008 Sep;71(1):71-5
            pubmed: 17920681
          18. Methods Enzymol. 1994;233:357-63
            pubmed: 8015470
          19. J Paediatr Child Health. 2007 Oct;43(10):667-72
            pubmed: 17854450
          20. Am J Physiol Regul Integr Comp Physiol. 2007 Nov;293(5):R1882-92
            pubmed: 17855494
          21. Physiol Rev. 1998 Apr;78(2):547-81
            pubmed: 9562038
          22. Equine Vet J. 2000 Sep;32(5):439-43
            pubmed: 11037267
          23. Vet Immunol Immunopathol. 1995 Mar;45(1-2):19-30
            pubmed: 7604535
          24. Am J Vet Res. 2003 Jan;64(1):83-7
            pubmed: 12518883
          25. Free Radic Biol Med. 2009 May 15;46(10):1411-9
            pubmed: 19268703
          26. Arthritis Res Ther. 2005;7(2):R380-91
            pubmed: 15743486
          27. Arthritis Rheum. 1979 Mar;22(3):251-9
            pubmed: 217393
          28. Pflugers Arch. 1989 Nov;415(2):127-35
            pubmed: 2556684
          29. Osteoarthritis Cartilage. 2003 Oct;11(10):747-55
            pubmed: 13129694
          30. J Biol Chem. 2009 Apr 3;284(14):9132-9
            pubmed: 19193642
          31. Nature. 2009 Apr 9;458(7239):780-3
            pubmed: 19194462
          32. Free Radic Biol Med. 2009 May 15;46(10):1386-91
            pubmed: 19268526
          33. Am J Vet Res. 2005 Jul;66(7):1238-46
            pubmed: 16111164
          34. Mol Pain. 2009 Jun 17;5:31
            pubmed: 19531269
          35. Am J Vet Res. 2007 Mar;68(3):290-6
            pubmed: 17331019
          36. Clin Chim Acta. 2003 Dec;338(1-2):99-105
            pubmed: 14637273
          37. Free Radic Biol Med. 1990;9(6):515-40
            pubmed: 2079232
          38. Clin Exp Rheumatol. 2005 Mar-Apr;23(2):205-12
            pubmed: 15895891
          39. Pharmacol Toxicol. 1996 Nov;79(5):247-53
            pubmed: 8936558
          40. Free Radic Biol Med. 2009 Mar 15;46(6):799-809
            pubmed: 19159678
          41. Food Chem Toxicol. 2009 Sep;47(9):2308-13
            pubmed: 19540899
          42. Gene Ther. 2002 Jan;9(1):12-20
            pubmed: 11850718
          43. Free Radic Biol Med. 2009 Jun 1;46(11):1472-7
            pubmed: 19272443
          44. Free Radic Res. 2003 Mar;37(3):257-68
            pubmed: 12688421
          45. Free Radic Biol Med. 2000 Jun 15;28(12):1745-50
            pubmed: 10946216
          46. Methods Enzymol. 1994;233:380-5
            pubmed: 8015473
          47. J Biol Chem. 1951 Nov;193(1):265-75
            pubmed: 14907713
          48. Free Radic Res Commun. 1992;17(5):299-311
            pubmed: 1483581

          Citations

          This article has been cited 3 times.
          1. Marković L, Vićić I, Lazarević Macanović M, Francuski Andrić J, Kovačević Filipović M, Radaković M. Degenerative Changes in MCP/MTP Joints of Working Horses Without Lameness: Integrating CT-Based Assessment and Synovial Fluid Biomarkers. Animals (Basel) 2025 Nov 24;15(23).
            doi: 10.3390/ani15233392pubmed: 41375451google scholar: lookup
          2. Tsuzuki N, Kanbayashi Y, Kusano K. Markers for oxidative stress in the synovial fluid of Thoroughbred horses with carpal bone fracture. J Equine Sci 2019 Mar;30(1):13-16.
            doi: 10.1294/jes.30.13pubmed: 30944542google scholar: lookup
          3. Celi P, Gabai G. Oxidant/Antioxidant Balance in Animal Nutrition and Health: The Role of Protein Oxidation. Front Vet Sci 2015;2:48.
            doi: 10.3389/fvets.2015.00048pubmed: 26664975google scholar: lookup
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