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Veterinary research forum : an international quarterly journal2012; 3(2); 97-101;

Antioxidant effect of different vitamins on methemoglobin production: An in vitro study.

Abstract: Nitrite intoxication occurs frequently in ruminants and equines. The most common treatment of this disorder is administration of 1% methylene blue, although the use of some antioxidant agents e.g. vitamins and complementary treatment may also be useful. The aim of this study was to evaluate the in vitro antioxidative effects of some vitamins on methemoglobinemia induced by sodium nitrite. For this purpose the blood sample of a healthy dairy cattle was pre-incubated with three different concentrations (5, 10, 20 mmol L(-1)) of each vitamin (E, C, B1, A and a combination of vitamin E and vitamin C) as antioxidant agent at 4 (°)C for 24 hours. A control group with normal saline instead of vitamin was applied. Then, all samples were treated with sodium nitrite (10 mmol L(-1)) as an oxidant agent for 10 minutes and the level of methemoglobin formation was measured spectrophoto-metrically. The results revealed that the level of methemoglobin decreased significantly (P < 0.05), when vitamin E (10 and 20 mmol L(-1)) and vitamin C (5 mmol L(-1)) was applied to the tests, separately. Vitamin C at the concentration of 20 mmol L(-1), was not effective, but it even increased methemoglobin formation significantly. Combination of vitamin E and C was significantly effective at concentration 5 mmol L(-1), but not at concentration 10 and 20 mmol L(-1). Vitamin A and vitamin B1 were not effective in any concentration. It was concluded that vitamins especially vitamin C and E can reduce oxidative effects which induced methemoglobin formation in vitro and could be used as an alternative medication.
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Publication Date: 2012-04-01 PubMed ID: 25653754PubMed Central: PMC4312803
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the impact of different vitamins on the production of methemoglobin, a type of hemoglobin with oxidized iron that can’t carry oxygen, which is often caused by nitrite poisoning in ruminants and equines.

Research Objectives

  • The aim of this research is to examine the in vitro (outside of living organisms) antioxidative capacities of various vitamins (E, C, B1, A) as a means to reduce or prevent methemoglobinemia, a condition characterized by elevated levels of methemoglobin in the blood, typically caused by nitrite intoxication.

Methodology

  • To test this, the blood sample of a healthy dairy cattle was pre-incubated with three different concentrations (5, 10, 20 mmol L(-1)) of each vitamin, including a combination of vitamin E and C, as antioxidant agent at 4 degrees Celsius for 24 hours.
  • A control group, where the blood was treated with normal saline instead of vitamins, was also established.
  • All samples were then exposed to sodium nitrite, serving as an oxidant agent, and the level of resulting methemoglobin was quantified spectrophoto-metrically (a method of measuring how much light the methemoglobin absorbs).

Findings

  • The results reveal that methemoglobin levels significantly dropped when treated with vitamin E (10 and 20 mmol L(-1)) and vitamin C (5 mmol L(-1)).
  • Interestingly, vitamin C at the concentration of 20 mmol L(-1), didn’t demonstrate effectiveness – it actually led to a notable rise in methemoglobin formation.
  • The combination of vitamin E and C was significantly effective at a concentration of 5 mmol L(-1) but didn’t show any effect at concentration 10 and 20 mmol L(-1).
  • Vitamin A and vitamin B1 did not affect methemoglobin formation, irrespective of their concentration.

Conclusions

  • The study concluded that vitamins, particularly vitamin C and E, can reduce the oxidative effects that lead to methemoglobin formation in vitro.
  • This suggests the potential for these vitamins to be used as alternative treatments for animals dealing with nitrite intoxication as opposed to the traditional administration of 1% methylene blue.

Cite This Article

APA
Atyabi N, Yasini SP, Jalali SM, Shaygan H. (2012). Antioxidant effect of different vitamins on methemoglobin production: An in vitro study. Vet Res Forum, 3(2), 97-101.

Publication

Veterinary research forum : an international quarterly journal
ISSN: 2008-8140
NlmUniqueID: 101625812
Country: Iran
Language: English
Volume: 3
Issue: 2
Pages: 97-101

Researcher Affiliations

Atyabi, Nahid
  • Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;
Yasini, Seyedeh Parastoo
  • Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;
Jalali, Seyedeh Missagh
  • Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;
Shaygan, Hamid
  • Faculty of Veterinary Medicine, Azad Islamic University, Garmsar branch, Garmsar, Iran.

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  3. Imani F, Behseresht A, Pourfakhr P, Shariat Moharari R, Etezadi F, Khajavi M. Prevalence of Abnormal Methemoglobinemia and Its Determinants in Patients Receiving Nitroglycerin During Anesthesia.. Anesth Pain Med 2019 Jun;9(3):e85852.
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  4. Das J, Katyal A, Naunwaar D. Dapsone-induced Methemoglobinemia in a Patient of Leprosy.. Indian J Dermatol 2015 Jan-Feb;60(1):108.
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