Antral follicle development and in-vitro maturation of oocytes from macaques stimulated with a single subcutaneous injection of pregnant mare’s serum gonadotrophin.
Abstract: A single s.c. injection of 1000 IU of pregnant mare's serum gonadotrophin (PMSG) stimulates the growth of multiple antral follicles in cynomolgus monkeys (Macaca fascicularis). The number of cumulus-enclosed oocytes (CEO) from six non-stimulated controls was 36 (mean = 6). In contrast, a total of 95 CEO (mean = 31.7) were recovered from three animals stimulated and ovariectomized 3 days later, while 385 CEO (mean = 128.3) were obtained from three animals stimulated and ovariectomized 4 days later. A comparison of the effects of highly purified human follicle-stimulating hormone (FSH), human luteinizing hormone (LH) and human thyroid-stimulating hormone (TSH) on cultures of rhesus monkey (Macaca mulatta) CEO cultured in medium 199 for 48 h showed that FSH and LH stimulated cumulus expansion but had moderate effects on the progression of meiosis. The progression that did occur seldom proceeded beyond the metaphase I stage. Human TSH had no demonstrable effect on cumulus expansion and meiotic progression. In other experiments FSH, LH and oestradiol-17 beta were added jointly to medium CMRL 1066 in which CEO from non-PMSG-stimulated and PMSG-stimulated cynomolgus monkeys were cultured for 48 h. If the CEO were obtained from non-PMSG-stimulated monkeys the hormones caused a significant cumulus expansion, but had only a limited effect on meiotic progression. However, if the CEO were obtained from PMSG-stimulated monkeys there was a significant stimulation of cumulus expansion and meiotic progression. The hormone treatment resulted in a yield of 72.8% metaphase II oocytes compared with 40.9% in the controls.
Publication Date: 1994-11-01 PubMed ID: 7868685DOI: 10.1093/oxfordjournals.humrep.a138404Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research examines the impact of pregnant mare’s serum gonadotrophin (PMSG) on antral follicle development and maturation of oocytes in macaques. The study found that a single injection of PMSG notably increased the amount of cumulus-enclosed oocytes (CEO), more so when the animals were stimulated for longer. Further tests using a trio of hormones on both PMSG-stimulated and non-stimulated CEO revealed a significant advancement in cumulus expansion and meiotic progression, particularly in PMSG-stimulated cases.
Findings on the Effects of a Single PMSG Injection
- The researchers injected a single subcutaneous dose of 1000 IU of PMSG in cynomolgus monkeys, triggering a surge in antral follicle development.
- For non-stimulated control monkeys, the total count of CEO was 36 (average 6). In contrast, for monkeys stimulated and ovariectomized after 3 days, the count jumped to 95 CEO (average 31.7), and for those stimulated and ovariectomized after 4 days, it was 385 CEO (average 128.3).
Comparative Analysis of Different Hormones
- The study also compared the effects of highly purified human follicle-stimulating hormone (FSH), human luteinizing hormone (LH), and human thyroid-stimulating hormone (TSH) on CEO cultures from rhesus monkeys.
- FSH and LH stimulated cumulus expansion but only slightly impacted meiotic progression, with progression rarely going beyond the metaphase I stage. TSH, on the other hand, showed no observable impact on either cumulus expansion or meiotic progression.
Findings from Joint Hormone Treatment
- In further experiments, FSH, LH, and oestradiol-17 beta were jointly added to CEO cultures from both PMSG-stimulated and non-stimulated monkeys.
- For non-PMSG-stimulated monkeys, these hormones triggered significant cumulus expansion but had only mild effects on meiotic progression.
- Contrastingly, in PMSG-stimulated monkeys, there was significant stimulation of both cumulus expansion and meiotic progression. Hormone treatment led to a yield of 72.8% metaphase II oocytes, compared to 40.9% in the controls.
Cite This Article
APA
Younis AI, Sehgal PK, Biggers JD.
(1994).
Antral follicle development and in-vitro maturation of oocytes from macaques stimulated with a single subcutaneous injection of pregnant mare’s serum gonadotrophin.
Hum Reprod, 9(11), 2130-2134.
https://doi.org/10.1093/oxfordjournals.humrep.a138404 Publication
Researcher Affiliations
- Division of Reproductive Biology, New England Regional Primate Research Center, Harvard Medical School, Southborough, MA 01772.
MeSH Terms
- Animals
- Culture Media
- Estradiol / pharmacology
- Female
- Follicle Stimulating Hormone / pharmacology
- Gonadotropins, Equine / pharmacology
- Luteinizing Hormone / pharmacology
- Macaca fascicularis
- Macaca mulatta
- Oocytes / drug effects
- Oocytes / physiology
- Ovarian Follicle / drug effects
- Ovarian Follicle / physiology
- Ovariectomy
- Thyrotropin / pharmacology
Grant Funding
- P51 PR00168 / OCPHP CDC HHS
- R24 PR02510 / OCPHP CDC HHS
- UO1 HD 21988 / NICHD NIH HHS
Citations
This article has been cited 2 times.- Albertini DF, McGinnis LK. A catalyst for change in reproductive science: John D. Biggers as a mentor’s mentor. J Assist Reprod Genet 2013 Aug;30(8):979-94.
- Cramer DW, Sluss PM, Powers RD, McShane P, Ginsburgs ES, Hornstein MD, Vitonis AF, Barbieri RL. Serum prolactin and TSH in an in vitro fertilization population: is there a link between fertilization and thyroid function?. J Assist Reprod Genet 2003 Jun;20(6):210-5.
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