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Application of liquid chromatography-mass spectrometry to the investigation of poisoning by Oenanthe crocata.
Abstract: Liquid chromatography-mass spectrometry (LC-MS) analysis of methanol extracts of Oenanthe crocata roots revealed that oenanthotoxin co-eluted with another major polyalkyne, 2,3-dihydro-oenanthotoxin, using the existing high performance liquid chromatography (HPLC) method (isocratic elution from C18 with aqueous methanol) for investigating Oenanthe poisoning. Positive ES or APCI gave [(M+H)-H(2)O](+) and its methanol adduct as major ion species for oenanthotoxin, whereas 2,3-dihydro-oenanthotoxin formed [M+H](+) and its methanol adduct. The two polyalkynes could be chromatographically resolved on C18 by gradient elution with aqueous acetonitrile. This provides superior analysis for oenanthotoxin using HPLC with photodiode array (PDA) detection alone, but for LC-MS/MS aqueous acetonitrile was less suitable due to poor ionisation and, with APCI, an increase in the relative abundance of a [M-1](+) species, which could confuse compound assignment. HPLC-PDA and LC-MS/MS methods using an aqueous acetonitrile or aqueous methanol mobile phase, respectively, were successful when applied to the analysis of the stomach contents of a pony suspected to have eaten O. crocata. Relevant product ion spectra, by ion trap MS/MS, accurate mass data and complete sets of (1)H and (13)C NMR spectral assignments are given for the two compounds.
Publication Date: 2006-05-26 PubMed ID: 16730246DOI: 10.1016/j.jchromb.2006.04.047Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The study explored the application of liquid chromatography-mass spectrometry (LC-MS) in the investigation of poisoning caused by Oenanthe crocata, which allowed for the detection and analysis of the toxins, namely oenanthotoxin and 2,3-dihydro-oenanthotoxin. The effective use of this method was demonstrated through testing the stomach contents of a pony suspected to have ingested O. crocata.
Liquid Chromatography-Mass Spectrometry (LC-MS) Application
- The researchers utilized LC-MS to analyze methanol extracts of Oenanthe crocata roots. They discovered that oenanthotoxin co-eluted with another major polyalkyne, 2,3-dihydro-oenanthotoxin.
- LC-MS represented a significant development in the process of investigating potential Oenanthe poisoning. This method offered a more efficient and accurate means of identifying the presence of toxins.
High Performance Liquid Chromatography (HPLC) Method
- The article mentioned the HPLC method as the existing approach to investigate Oenanthe poisoning. However, the new LC-MS method was found to provide superior results, indicating its potential as a replacement for the previously used method.
- Even though the HPLC method with photodiode array (PDA) detection was effective for analyzing oenanthotoxin, the LC-MS/MS method was less suitable given the poor ionization and an increase in the relative abundance of a [M-1](+) species.
Investigation Application and Results
- The researchers applied the HPLC-PDA and LC-MS/MS methods to analyze the stomach contents of a pony suspected of eating O. crocata. Both methods were successful, affirming their potential applicability in Oenanthe poisoning investigations.
- The research gathered relevant product ion spectra, ion trap MS/MS, accurate mass data, and complete sets of (1)H and (13)C NMR spectral assignments for the two compounds. This collection of data further validated the application of LC-MS in detecting and analyzing toxins in Oenanthe crocata.
Cite This Article
APA
Kite GC, Stoneham CA, Veitch NC, Stein BK, Whitwell KE.
(2006).
Application of liquid chromatography-mass spectrometry to the investigation of poisoning by Oenanthe crocata.
J Chromatogr B Analyt Technol Biomed Life Sci, 838(1), 63-70.
https://doi.org/10.1016/j.jchromb.2006.04.047 Publication
Researcher Affiliations
- Royal Botanic Gardens Kew, Richmond, Surrey TW9 3AB, UK. g.kite@kew.org
MeSH Terms
- Alkynes / analysis
- Alkynes / chemistry
- Alkynes / poisoning
- Animals
- Chromatography, High Pressure Liquid / methods
- Enediynes
- Fatty Alcohols / analysis
- Fatty Alcohols / chemistry
- Fatty Alcohols / poisoning
- Hazardous Substances / analysis
- Hazardous Substances / poisoning
- Horse Diseases / chemically induced
- Horse Diseases / diagnosis
- Horses
- Magnetic Resonance Spectroscopy
- Methanol / chemistry
- Molecular Structure
- Oenanthe / chemistry
- Plant Roots / chemistry
- Reproducibility of Results
- Sensitivity and Specificity
- Spectrometry, Mass, Electrospray Ionization / methods
- Stereoisomerism
Citations
This article has been cited 1 times.- Annibal A, Schubert K, Wagner U, Hoffmann R, Schiller J, Fedorova M. New covalent modifications of phosphatidylethanolamine by alkanals: mass spectrometry based structural characterization and biological effects. J Mass Spectrom 2014 Jul;49(7):557-69.
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