Application of the restricted-access precolumn packing material alkyl-diol silica in a column-switching system for the determination of ketoprofen enantiomers in horse plasma.

The research study applies alkyl-diol silica sorbents, a type of restricted-access material beneficial for direct biofluid processing, in a column-switching system to estimate ketoprofen enantiomers in horse plasma without needing sample clean-up.

Introduction and Background

• In this paper, the researchers expanded upon previous work related to the use of LiChrospher ADS (alkyl-diol silica) sorbents. These are a part of restricted-access materials designed for unique precolumn packings in liquid chromatography (LC).
• The advantage of these sorbents is their ability for direct injection of biofluids without needing sample clean-up.
• Simultaneously, these sorbents allow the elimination of the protein matrix while achieving a quantitative recovery.

Methodology

• The researchers used UV detection at 260 nm for ketoprofen determination.
• Plasma samples were introduced to the ADS precolumn using a 0.1 M phosphate buffer at pH 7.0.
• After rinsing the column with the buffer, the ADS column was backflushed with a mobile phase consisting of 0.01 M phosphate buffer-6% (v/v) 2-propanol-5 mM octanoic acid at a pH of 5.5.

Key Application

• The mobile phase transports the analytes to the chiral-HSA (human serum albumin) column, achieving the separation of ketoprofen enantiomers.
• The researchers were able to achieve this with a resolution factor of 1.4.

Significance and Applicability

• Importantly, the developed column-switching method was found to be fully applicable to plasma injections. This new approach can lead to more efficient and streamlined drug testing and biological sample analysis.