Application to cows and horses of Spotchem, a dry-chemistry blood analyzer for use in veterinary clinics.

The research illustrates the application of a dry-chemistry blood analyzer, Spotchem SP-4410, in analyzing blood chemistry of cows and horses. The analyzer was found to be effective, indicating that it could be useful in veterinary clinics.

Objectives of the Research

• The study aimed to explore the applicability and effectiveness of the Spotchem SP-4410 dry-chemistry blood analyzer for understanding equine and bovine blood chemistry. It seeks to establish whether it can be used as a standard tool for veterinary clinics.

Methodology

• To conduct the research, various factors were quantified in bovine serum including total protein (TP), albumin (Alb), total bilirubin (T-Bil), blood urea nitrogen (BUN), total cholesterol (T-Cho), glucose (Glu), calcium (Ca), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), creatinine phosphokinase (CPK), and alkaline phosphatase (ALP).
• Each sample was analyzed using both the SP-4410 and an automated blood analyzer, representing a conventional wet-chemistry reference system.
• Regression analysis was used to examine the data gathered. Specifically, comparison and correlation were based on factors like the correlation coefficient, the coefficient of variation, and the range of normal reference values.

Results

• Findings indicate a high correlation between the readings of the SP-4410 and wet-chemistry analyzer. AST showed the highest correlation coefficient of 0.997, with all correlation coefficients being 0.93 or higher. This suggests the dry-chemistry analyzer is reliable for testing these blood parameters.
• The coefficient of variation was lower than 5.0 except in the case of bovine T-Bil where it was 5,756, suggesting the analyzer provides consistent results except for the test for total bilirubin in bovine blood.
• The ranges of normal reference values as measured by the SP-4410 were found to be in line with the values reported by other researchers, with a few exceptions such as GGT and CPK, which were slightly higher.
• The most significant interference, or factor affecting the accuracy of test results, was observed with hemoglobin.

Conclusion

• Based on the findings, this research concluded that a dry-chemical analysis of blood serum is practical and effective when using the SP-4410, making it a viable tool for analyzing both bovine and equine blood in veterinary clinics.