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Archives of virology1988; 101(3-4); 183-190; doi: 10.1007/BF01310999

Asinine herpesvirus genomes: comparison with those of the equine herpesviruses.

Abstract: Two previously unknown and distinct herpesviruses were isolated from donkeys. One, with the characteristics of a betaherpesvirus, was isolated from the leukocytes of an apparently healthy donkey, while the second, an alphaherpesvirus, was recovered from the nasal cavity of donkeys given high doses of corticosteroids, and caused rhinitis in two seronegative weanling donkeys when they were intranasally infected. Few, if any, restriction endonuclease fragments were shared by the donkey betaherpesvirus, equine herpesvirus 2 (EHV 2) or EHV 5, a second distinctly different equine betaherpesvirus, nor by the donkey alphaherpesvirus, EHV 1, EHV 4, or EHV 3. In Southern blot analysis the donkey betaherpesvirus showed low levels of sequence similarity to both EHV 2 and EHV 5, while the donkey alphaherpesvirus and EHV 1 shared a moderate degree of sequence similarity, less similarity with EHV 4 and very low level of sequence similarity with EHV 3. These two isolates appear prototypic of two previously unrecorded herpesviruses for which the names asinine herpesvirus 2 and 3 are suggested for the betaherpesvirus and the alphaherpesvirus respectively.
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Publication Date: 1988-01-01 PubMed ID: 2845891DOI: 10.1007/BF01310999Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study uncovered two previously unknown types of herpesvirus found in donkeys. One type showed characteristics of a betaherpesvirus and was found in a healthy donkey, while the second type was an alphaherpesvirus found in donkeys’ nasal cavities after they received high doses of corticosteroids, causing rhinitis. The researchers proposed the names ‘asinine herpesvirus 2’ for the betaherpesvirus and ‘asinine herpesvirus 3’ for the alphaherpesvirus.

Discovering New Herpesviruses

  • The researchers managed to isolate two previously unknown and distinct herpesviruses from donkeys.
  • One of these viruses displayed features of a betaherpesvirus, a subfamily of the herpesviridae family. This virus was found within the leukocytes, or white blood cells, of a seemingly healthy donkey.
  • The other isolated virus bore characteristics of an alphaherpesvirus, another subfamily of herpesviridae, and was found in the nasal cavities of donkeys who had been given high doses of corticosteroids.

Effects and Analysis

  • This alphaherpesvirus proved to be pathogenic, causing a condition known as rhinitis (inflammation of the mucous membrane of the nose) in two young, seronegative donkeys when it was introduced to their noses.
  • To try and determine the relationship between these new viruses and known equine herpesviruses, the researchers conducted a series of tests. They found that the new betaherpesvirus shared very few, if any, restriction endonuclease fragments with equine herpesvirus 2 (EHV 2) or EHV 5. The new alphaherpesvirus was similarly distinct from EHV 1, EHV 3, and EHV 4.
  • A Southern blot test was used to further inspect these relationships, showing that the donkey betaherpesvirus has a low level of sequence similarity to both EHV 2 and EHV 5. The donkey alphaherpesvirus had a moderate level of sequence similarity to EHV 1, less similarity to EHV 4, and a very low level of sequence similarity to EHV 3.

Proposal of New Herpesvirus Names

  • Because of their novel characteristics and their distinctness from other known herpesviruses, the researchers proposed naming these newly discovered viruses after their donkey hosts. They suggested ‘asinine herpesvirus 2’ for the betaherpesvirus and ‘asinine herpesvirus 3’ for the alphaherpesvirus, introducing these names into the virological nomenclature.

Cite This Article

APA
Browning GF, Ficorilli N, Studdert MJ. (1988). Asinine herpesvirus genomes: comparison with those of the equine herpesviruses. Arch Virol, 101(3-4), 183-190. https://doi.org/10.1007/BF01310999

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 101
Issue: 3-4
Pages: 183-190

Researcher Affiliations

Browning, G F
  • School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Ficorilli, N
    Studdert, M J

      MeSH Terms

      • Animals
      • Blotting, Southern
      • DNA, Viral / analysis
      • DNA, Viral / genetics
      • Herpesviridae / classification
      • Herpesviridae / genetics
      • Herpesviridae / isolation & purification
      • Herpesviridae Infections / microbiology
      • Herpesviridae Infections / veterinary
      • Perissodactyla / microbiology
      • Sequence Homology, Nucleic Acid
      • Species Specificity

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