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Home / Equine Research Bank / Vet Clin Pathol / Assay variability and storage stability of the myeloperoxida...
Veterinary clinical pathology2021; 50(1); 28-36; doi: 10.1111/vcp.12981

Assay variability and storage stability of the myeloperoxidase index of the ADVIA 2120i hematology analyzer in canine and equine whole blood samples.

Abstract: The myeloperoxidase index (MPXI), on ADVIA hematology analyzers, reflects the mean neutrophil myeloperoxidase staining. It is used as a marker of inflammation in animals and people, but assay variability and storage stability are unknown. Objective: We aimed to determine MPXI precision and stability with refrigerated storage of canine and equine EDTA-anticoagulated blood and compared MPXI results between two analyzers. Methods: Inter-assay coefficients of variations (CVs) were determined from three human-based controls assayed before and after a 20- or 21-day calibration. Blood from 14-16 dogs and 26 horses was assayed 4-10 times within 1 day for intra-assay CV measurements. Median control and single run results from 18 canine and 35 equine samples were compared between analyzers. Blood from 10-12 dogs and 10-11 horses was analyzed after collection, and 24, 48, and 72 hours of refrigerated storage. Results: Inter-assay CVs of controls were 10.7%-15.9% and 6.4%-9.6% before and 4.3%-7.7% and 2.8%-17.5% after calibration, for ADVIA 1 and 2, respectively. Calibration altered peroxidase gain settings and improved precision. Intra-assay CVs were 0.6%-64% and 3%-350% for canine and equine samples, respectively. Median MPXI results differed significantly between the analyzers, likely from calibration-associated changes in gains. MPXI decreased with storage, and with variable changes between animals and analyzers. Platelet clumps and lipid contributed to the variability in replicate MPXI measurements. Conclusions: MPXI has a higher variability in equine samples than in canine samples. Equivalent results might not be obtained between analyzers. Results change unpredictably with repeated analyses over 72 hours. MPXI measurements might only be useful in controlled research settings.
© 2021 American Society for Veterinary Clinical Pathology.
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Publication Date: 2021-03-02 PubMed ID: 33655602DOI: 10.1111/vcp.12981Google Scholar: Lookup
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Summary

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The research article investigates the accuracy and reliability of the myeloperoxidase index (MPXI), a marker of inflammation, in dog and horse blood samples using ADVIA 2120i hematology analyzers. The study specifically focuses on the potential variability in the results and the stability of the results during refrigerated storage.

Research Methods

  • The researchers examined inter-assay coefficients of variation (CVs) from three human-based controls tested before and after a 20 or 21-day calibration period.
  • Blood samples from 14-16 dogs and 26 horses were tested between 4 to 10 times in a day to measure intra-assay CV.
  • Median control and single-run results from 18 canine and 35 equine samples were compared between two analyzers.
  • For storage stability assessment, blood from 10-12 dogs and 10-11 horses was analyzed at the time of collection, and then after 24, 48, and 72 hours of refrigerated storage.

Key Findings

  • Inter-assay CVs of control samples were between 10.7%-15.9% and 6.4%-9.6% before calibration, and 4.3%-7.7% and 2.8%-17.5% after calibration, for ADVIA 1 and 2, respectively. The results indicate that calibration improved precision.
  • Intra-assay CVs ranged from 0.6%-64% for canine samples and 3%-350% for equine samples showing a higher variability in equine samples.
  • Notably, the MPXI results differed significantly between different analyzers, likely due to calibration-associated changes in the gain settings, and decreased during refrigerated storage.
  • Other factors such as platelet clumps and lipid contributed to the variability in replicate MPXI measurements.

Conclusions

  • The study concludes that the myeloperoxidase index (MPXI) displays higher variability in horse samples compared to dog samples.
  • Evidence was also obtained to suggest that equivalent results might not be achieved when different analyzers are used.
  • Additionally, the index results appear to change unpredictably over a 72-hour period with repeated analyses.
  • Given these findings, the use of MPXI measurements might be limited to controlled research settings.

Cite This Article

APA
Chu SAA, Stokol T. (2021). Assay variability and storage stability of the myeloperoxidase index of the ADVIA 2120i hematology analyzer in canine and equine whole blood samples. Vet Clin Pathol, 50(1), 28-36. https://doi.org/10.1111/vcp.12981

Publication

Veterinary clinical pathology
ISSN: 1939-165X
NlmUniqueID: 9880575
Country: United States
Language: English
Volume: 50
Issue: 1
Pages: 28-36

Researcher Affiliations

Chu, Shelley Ann Ash
  • Department of Population Medicine and Diagnostic Science, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
Stokol, Tracy
  • Department of Population Medicine and Diagnostic Science, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.

MeSH Terms

  • Animals
  • Dog Diseases / diagnosis
  • Dogs
  • Hematologic Tests / veterinary
  • Hematology
  • Horse Diseases
  • Horses
  • Inflammation / veterinary
  • Neutrophils
  • Peroxidase

References

This article includes 16 references
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Citations

This article has been cited 1 times.
  1. Fingerhut L, Yücel L, Strutzberg-Minder K, von Köckritz-Blickwede M, Ohnesorge B, de Buhr N. Ex Vivo and In Vitro Analysis Identify a Detrimental Impact of Neutrophil Extracellular Traps on Eye Structures in Equine Recurrent Uveitis.. Front Immunol 2022;13:830871.
    doi: 10.3389/fimmu.2022.830871pubmed: 35251020google scholar: lookup
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