Assessment of a reflectance photometer in a veterinary laboratory.
Abstract: This report is an assessment of clinical chemistry dry reagent methodology for veterinary use. A portable reflectance photometer and dry reagent strips were used to measure canine whole blood hemoglobin, and total bilirubin, glucose, cholesterol, creatinine and urea in canine, bovine, equine and feline sera. Creatine kinase and lactate dehydrogenase were assayed in canine, bovine and equine sera. The following aspects of performance are reported: within run variation determined on canine samples, between run variation using a commercial control, correlations between dry reagent and wet reagent methodology on clinical samples, and dry reagent method serum chemistry reference values for the cow, horse and dog. A brief description of some technical advantages and limitations is included.Technical requirements were minimal while reproducibility and accuracy compared well with the wet reagent method. The dry reagent method was found to be suitable for determination of canine, bovine, equine and feline serum variables as listed above.
Publication Date: 1984-06-01 PubMed ID: 17422414PubMed Central: PMC1790592
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- Journal Article
Summary
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This research article explores the effectiveness of a portable reflectance photometer and dry reagent strips in assessing specific aspects of animal blood and serum, and the results confirm the suitability of this method for use in a veterinary context.
Objective and Tools of the Research
- The main objective of the research was to evaluate the effectiveness of ‘Clinical Chemistry Dry Reagent’ methodology for veterinary use.
- The researchers used a portable reflectance photometer and dry reagent strips as their primary tools for conducting their assessments.
- They tested these tools on a variety of animals including canines, bovines, equines, and felines.
Assessments Conducted and Variables Tested
- The researchers conducted a variety of tests, studying different parameters in animal blood and serum.
- In canine whole blood, they measured hemoglobin, total bilirubin, glucose, cholesterol, creatinine, and urea.
- In sera from various animals, the researchers tested creatine kinase and lactate dehydrogenase.
Evaluation and Outcomes
- The performance of the dry reagent system was assessed on multiple fronts. These include within-run variations on canine samples, between run variations using commercial control, and correlations between dry reagent and wet reagent methodologies on clinical samples.
- One of the positive outcomes was the determination of serum chemistry reference values for cows, horses, and dogs using the dry reagent method.
- The technical requirements for the dry reagents method were found to be minimal. However, the reproducibility and accuracy of this method were found to be comparable to the traditional wet reagent method.
- The researchers concluded that the dry reagent method is suitable for determining serum variables in various animals including dogs, cows, horses, and cats.
Advantages and Limitations of the Method
- The research also included an exploration of the technical advantages and limitations of the dry reagent method.
- While the specifics of the advantages and limitations are not listed in the abstract, the implication is that the benefits must outweigh any drawbacks given the conclusion that this method is suitable for broad-ranging veterinary use.
Cite This Article
APA
Belford CJ, Lumsden JH.
(1984).
Assessment of a reflectance photometer in a veterinary laboratory.
Can Vet J, 25(6), 243-246.
Publication
Researcher Affiliations
References
This article includes 5 references
- Robertson SA, Lucke JN, Hall GM. Blood glucose measurement: an evaluation of a small reflectance photometer under field conditions.. Vet Rec 1982 Dec 11;111(24):557.
- Church DB, Watson AD. Whole blood glucose determination in dogs using dextrostix and the eyetone reflectance colorimeter.. J Small Anim Pract 1979 Mar;20(3):163-8.
- Lumsden JH, Mullen K. On establishing reference values.. Can J Comp Med 1978 Jul;42(3):293-301.
- Stewart TC. Evaluation of a reagent-strip method for glucose in whole blood, as compared with a hexokinase method.. Clin Chem 1976 Jan;22(1):74-8.
- Schersten B, Kuhl C, Hollender A, Ekman R. Blood glucose measurement with Dextrostix and new reflectance meter.. Br Med J 1974 Aug 10;3(5927):384-7.
Citations
This article has been cited 1 times.- Papasouliotis K, Tennant KV, Dodkin S, Mason J. Comparison of Measurements of 12 Analytes in Equine Blood Samples Using the In-Practice Falcor 350 and the Reference KoneLab 30i Analysers.. ISRN Vet Sci 2012;2012:475419.
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